All issues
Volume 43 (1991)
- Issue 6 Pages 591-
- Issue 5 Pages 513-
- Issue 4 Pages 347-
- Issue 3 Pages 191-
- Issue 2 Pages 117-
- Issue 1 Pages 1-
Volume 43, Issue 5
Displaying 1-4 of 4 articles from this issue
- |<
- <
- 1
- >
- >|
-
Yuichiro NAKAI, Yoshihiko MIYAZAKI, Yasunaga MATSUOKA, Shigeki MATSUO, ...1991 Volume 43 Issue 5 Pages 513-518
Published: September 01, 1991
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSWe retrospectively examined the perinatal course of 12 fetuses observed having distinguished or reversed end-diastolic flow in the umbilical artery or the fetal descending aorta.
Eleven of the 12 fetuses were delivered by Caesarian section due to fetal distress with Apgar scores of less than 7 points at 1 minutes.
In 7 of these cases, we observed reversed flow in the umbilical artery or the fetal descending aorta. Of these 7 cases, there were 4 cases were delivered with severe asphyxia with Apgar scores of less than 5 points at 1 minute. Two of these cases died within 7 days after delivery.
In the 5 cases who were observed having only distinguished flow, none died in utero nor after delivery. Three were at less than 5 Apgar points at 1 minute, but at 5 minutes were at 8.4 ± 1.7 (mean ± SD), compared with those having reversed end-diastolic flow, which were at 7.4 ± 1.3 at 5 minute.
As the outcome of neonates observed with reversed flow in the umbilical artery or descending aorta is poor, an immediate delivery is necessary. Moreover, before the 33rd gestational week, the outcome of neonates observed the reversed flow is quite poor. We must consider termination of the pregnancy, when only distinguished end-diastolic flows are observed.
We found Doppler flow velocimetry to be useful tool in diagnosis of pregnancy termination, especially in cases before the 33rd gestational week.View full abstractDownload PDF (5468K) -
Hideaki TOHJI1991 Volume 43 Issue 5 Pages 519-527
Published: September 01, 1991
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSIn order to study the protein molecules involved in sperm-egg interactions, we established a hybridoma which secreted a fertilization-blocking monoclonal antibody directed against human spermatozoa. Sperm membrane-associated proteins were extracted from ejaculated human sperm by sonication and mixed with Freund's complete abjuvant to immunize CBA/N mice. Spleen cells from the immunized mice were fused with mouse myeloma cells (NS-1) by the standerd method, and the culture supernatants of the hybridoma cells were screened for antibody production against sperm membrane proteins by ELISA. The antibody-positive hybridomas were examined for their blocking effect on human sperm penetration of zona-free hamster eggs (hamster test).
A hybridoma (3B10) which secreted antibody reactive to human sperm membrane proteins and had a strong blocking effect in the hamster test was established. The monocloal antibody (MAb-3B10) showed a strong reaction with capacitated human spermatozoa by immunofluorescence staining and blocked human sperm-egg interactions. The molecular size of the antigen corresponding to MAb-3B10 was determined to be 73RD by Western bloting. The corresponding antgenic molecules were isolated from sperm membrane proteins by gel filtration and reverse-phase HPLC, and the N-terminal amino acid sequence of the isolated antigen was determined. The N-terminal 20 amino acid sequence was found to ho identical to that of human lysozyme. We then raised antiserum to purified human lysozyme in mice and found that the anti-lysozyme antibody also had a strong blocking effect in the hamster test.
There results suggested that a lysozyme-like substance associated with human spermatozoa may play an important role in sperm-egg interactions.View full abstractDownload PDF (6867K) -
Tatsuo SUDO, Akira TAKENAKA, Yasuhiko YOKOE, Ichiro YAMADE, Yoshiki TA ...1991 Volume 43 Issue 5 Pages 528-533
Published: September 01, 1991
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSSerum CA-125 levels were analyzed in 40 patients with sterility including 25 cases of endometriosis externa (EN) and 15 cases of non-endometriosis in order to evaluate its usefulness in the diagnosis of EN. (The diagnosis was confirmed by laparoscopy). Serum CA-125 levels during non-menstrual periods were significantly elevated only in patients with EN of stage III/IV, as compared with those in non-endometriosis. However, CA-125 levels during menstruation (days 1-2 of the menstrual cycle) exceeded 35 units/ml even early stage of EN and these were significantly higher than those in women without endometriosis. The result suggested the measurement of CA-125 during menstruation was more useful for diagnosis of EN than those in non-menstrual periods. An acception occured in 7 cases of 25 cases without endometriosis, where serum CA-125 levels during menstruation exceeded 35 units/ml.These cases supposedly complicated with peritoneal stimulations by pelvic adhesion, ascites and inflammation.
Laparoscopy is still required to confirm the diagnosis of endometriosis, but, serum CA-125 level during the menstrual periods could greatly improve the usefulness of the diagnosis.View full abstractDownload PDF (730K) -
Koichi TERAKAWA, Yoshiyuki IHARA, Masazumi TWAI, Koji HASHII, Ikuo KON ...1991 Volume 43 Issue 5 Pages 534-539
Published: September 01, 1991
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSIntestinal obstruction during pregnancy is rare. Its symptoms are similar to those physiologically associated with pregnancy and it is difficult to perform physical examination due to the enlarged gravid uterus. Thus, the diagnosis is likely to be late, the timing of surgery is likely to be late, and the prognosis of the mother and baby tends to be poor. Three cases of intestinal obstruction during pregnancy in our department are reported. The etiologies of the 3 cases were volvulus secondary to malrotation, colon cancer and postoperative adhesions, respectively. Each case was refered to us from other obstetricians and underwent operation. Only in the third case did both the mother and baby survive. When we examine a pregnant woman with severe abdominal pain, nausea and constipation, we should always keep intestinal obstruction in mind. Especially when the patient has a history of a previous abdominal operation, we should strongly suspect intestinal obstruction secondary to adhesions. It is important for patients with intestinal obstruction during pregnancy to be diagnosed early and to undergo operation within an adequate time period.View full abstractDownload PDF (5291K)
- |<
- <
- 1
- >
- >|