This research work investigated the effect of low oxygen and high carbon dioxide atmosphere on the respiration rate and polyphenoloxidase (PPO) activity that were the main factors in fresh shiitake quality decay. Fresh Shiitakes were stored in CA conditions conducted in 2 research periods, i. e. CO
2: 20% mix to O
2: 10%, 5%, and 1%; and O
2: 1% mix to CO
2: 5%, 10%, and 15%; normal air treatment as control, and temperature 0°C and 5°C.
Decreasing O
2 concentration to 1% and/or increasing CO
2 to 20% decreased the respiration rate more than normal air during storage period. Both of given gas treatments were either the lower the O
2 concentration or the higher the CO
2 concentration the lower the respiration rates. Moreover RQ was founded close to 1 in normal air treatment after first fifth day, however RQ was less than 1 for given gas treatment. PPO Activity increased in normal air and decreased in gas treatments during storage. Discrimination of CO
2 concentrations in constant low O
2 concentration have firm effect on PPO activity in fresh shiitakes than that's discrimination of O
2 in high CO
2 concentration. Given gas treatments hold real pH of fresh shiitake did not decrease during storage.
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