Bacth methane fermentation from acetic acid was carried out with acclimated-methanogens using stopped culture tube at pH 7 and 37°C. The effects of trace metals solution added to the medium for methane formation from acetic acid were investigated in two cases, lower initial cell density of methanogens (0.06g/L) and higher initial cell density of methanogens (0.6g/L). Optimum trace metals solution for methane formation rate were obtained on both cases. At early stage of the tube cultivation, the maximum methane formation rate was obtained at 0.1mL/L of trace metals solution for lower initial cell density. As cultivation time was elapsed, the trace metals solution concentration that maximum methane formation rate obtained was changed to higher than that of 0.1mL/L. In contrast with the case of 0.1mL/L of trace metals solution, methane formation rate was doubled at 10mL/L of trace metals solution after 16 days of cultivation. However, in the case of a higher initial cell density, the maximum methane formation rate observed at higher concentration of trace metals solution concentration of 15mL/L, which is 150 times greater than that of previous report. Inhibition of methane formation at high concentration of trace metals solution (more than 15mL/L) was observed at early stage. As cultivation time was elapsed, the inhibition with the high concentration of trace metals decreased. During 2 to 7 days of cultivation, the rate of methane formation was 3.7 times higher than that of 0.1mL/L of trace metals solution concentration.
It was found that inhibition due to the high concentration of trace metals solution depended on inoculation size of methanogens, and high rate of methane formation can be obtained by optimizing the concentration of trace metals solution added to the medium.
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