1) Employing Heiderberger's method, the author studied quantitative precipicin reactions in electrophoretically simple antigen systems, (yeast polysaccharide, crystalline hens egg alubumin, crystalline horse serum albumine, bovine serum and pseudoglobulin, bovin thyroglobulin and Proteus X19 Systems).
2) The antibody/antigen ratio in equivalence zone obtained by the immunological method by means of succesive injection of a small quantity of antigen, was higher than that obtained by the normal immune method.
3) The reaction equations were characteristic in each of the antigens, I was able to divide them into polysaccharide, albumin, globulin and bacteria systems.
Generally, the ratio AbN/Ag N in the precipitate in equivalence zone showed a tendency to be larger the larger the molecular wight of the antigens.
4) Calculating the molecular ratio of specific precipitate in equivalence zone, by means of antibody N/antigen N which I measured, and also using the known molecular weight of antigen and antibody, we obtained the following results,
Crystalline hens egg albumin 2.7
Crystalline horse serum albumin 4.6
Bovine thyroglobulin 12.2
5) I found that antibody quantity calculated by quantitative precipitin method agreed with antibody quantity calculated by electrophoretic method, and we measured antibody quantity in immune serum globulin by two different methods.
The results were as follows.
Yeast polysauharide; 18.6-30.5%, crystalline hens egg albumin: 17.5-34.9, crystalline harse serum albumin; 25.4-34.1%, γ-pseudo globulin; 21.1%, and Thyroglobulin; 19.7%.
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