生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
1 巻, 2 号
選択された号の論文の13件中1~13を表示しています
  • 平井 秀松
    1951 年 1 巻 2 号 p. 83-89,140
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
  • 稻垣 博, 西島 安則, 東 省三, 永井 孝司
    1951 年 1 巻 2 号 p. 90-96
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
    Lamm's scale method for the diffusion measurement is based on the gradient of refractive index along the diffusion column, which affords displacement of the scale line attached to the front of the diffusion cell. The authors described its theoretical foundation and methods to calculate the diffusion constant in the case where the Lamm's scale method was adopted. The calculations mentioned above give the conclusion that the diffusion curve (concentration gradient do/dx vs. x) takes theoretically the Gaussian error function, in the case where the diffusion obeys the Fick's law. However, most of the diffusion curves measured on high polymer solutions do not show such Gaussian curve. Therefore, the authors disscussed the reason why such an anomaly would happen.
    (i) Diffusion constant of high polymer depends on the concentration in which the constant is measured. Such dependency shows the skewness in the diffusion curve, having a long tail exterding towards increasing of the concentration. Therefore, when the skew curve is obtained, the diffusion constant Dm, which is caluculated by the “moment” method, cannot be used to determine the molecular weight. combining with the sedimentation constant. Accordingly, it is important to derive a formula to estimate the diffusion constant in the infinite dilution. The authors introcuced the formula derived by N. Gralen (1944) and also showed another dbtained by one of the authors.
    (ii) The polydispersity of a high polymer affords an anomalous kurtosis to the normal curve. The mathematical treatment in such a case may give the information on the distribution of a molecular weight in the sample.
    As a conclusion, the authors emphasized that the Lamm's method would be more advantageous than other methods, especially the “Schlieren” method, which have been used by many workers recently.
  • 高橋 基之, 林 靖
    1951 年 1 巻 2 号 p. 97-100,119
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
    リンパ腺のアルカリ性燐酸酵素は,腺の生理鹽水抽出液の中に含まれる。抽出液をpH4.5とした濾液をアルコールによつて分割し,更に之を水によつて抽出すれば,この中へ抽出される。各分屑を電氣泳動及び泳動對流によつて處理することにより,燐酸酵素が易動度小なるGlobulin中に含まれることを推定し得た。
  • 鹽析法,Convective Electrophoresisに依るγ-globulin分劃の研究(免疫化學研究第1報)
    緒方 正名
    1951 年 1 巻 2 号 p. 101-107,140
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
    1. It was observed that the hyper immune rabbit serum showed an increase in the total protein concentration and γ-globulin concentration relative to the normal rabbit serum.
    2. The results of my investigation of the supernatant of antigen-antibody reactions by means of electrophoresis, showed that the γ-globulin of immune rabbit serum precipitated only partially by antigen-antibody reactions.
    By examination of the supernatant by means of antigen-antibody reactions, a remarkable decrease in the titer of antibodies was ascertained. The partial (17 to 33%) precipitation of the γ-globulin of immune rabbit serum could be demonstrated by the fact that globulin concentration estimated by means of electrophoresis was greater than protein concentration precipitated by quantitative precipitin reaction. It was to be inferred therefrom that γ-globuin of immune rabbit serum contained antibadies feeble in reactive power in addition to the antibodies of high potency.
    3. I studied quantitative precipitin reactions and quantitative complement fixation reaction in crystalline egg albumin systems.
    4. Antigen-antibody reaction by monomolecular layer method was found to be a sensitive test for cross reaction (bovine serum albumin and globulin system).
    Monomolecular layer of antigen absorbed complement.
    Monomolecular films can be available for the measurement of area of badily surface.
    5. Study on the fractionation of γ-globulin.
    a) Fractionation by ammonium sulfate. Electrophoretically homogeneous globulin way obtained by means of the rotating membrane method in which γ-globulin was salted out at the concentration of 1.39M. 1.86M and 1.22M (three times) of ammonium sulfata
    b) γ-Globulin of 90 to 95% purity was isolated in the process of twofold direct purification of original serum by convective electrophoresis. When isolated by the above method, albumin and globulin showed different immunological specificity as antigen.
    By convective electrophoresis, I could further purify the samples that was isolated by the ethanol fractionation. (Cohn II, III)
  • 近 新五郎, 浦田 卓, 木村 武
    1951 年 1 巻 2 号 p. 108-109,139
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
    正常人血清のγ1-globに關する2,3の基礎的實驗を行い,次の結果を得た。
    1) γ1-globは上昇測で分離しやすく,最も早い分離は泳動60分後に見られる。
    2) γ1-globの相對的易動度は35~48で,その%の平均値は2.5である。
    3) γ1-globの最も分離しやすい泳動條件は蛋白濃度1.5~2.0g/dl,泳動時間120分である。なおdiagonal slitを60°近くにすることが望ましい。
    4) γ1-globが明確な峰を形成しない時は,β峰とγ峰との分離垂線の下し方知何によつて,β-及びγ-globの%は數パーセントの變動をみる惧れがある。
  • 滲出性肋膜炎患者に於ける觀察
    松岡 正俊
    1951 年 1 巻 2 号 p. 110-112
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
    In order to make clear the relationship between erythrocyte sedimentation rate and palsma protein, electrophoretic fractions of plasma of 5 cases of pleurisy were measured by Tiselius apparatus 31 times. The results are as follows:
    1. Erythrocyte sedimentation rate in pleurisy is significantly correlative with total protein, α-globulin β-globulin and γ-globulin concentrations and is not significantly correlative with albumin and fibrinogen concentrations.
    2. The linear regression function of erythrocyte sedimentation rate to plasma proteinfractions is calculated to be,
    y=-10.47xA+72.60xα+45.04xβ-24.81xφ+44.87γ-62.5
    where
    y: erythrocyte sedimentation rate (mm/hour)
    xi: concentration of component “i” (g./dl.)
    a) Partial regression coefficients of α-, γ- globulins are sigmficart.
    b) The difference between partial regression coefficient of albumin and that of α-globulin is significant.
    It is the same with the difference between partial regression coefficient of albumin and that of γ-globulin.
    3. Existence of certain influential elements to erythrocyte sedimentation rate of pleuritic patients is presumed.
  • 豫報
    前川 孫二郎, 熊谷 直家, 荒木 仁, 中澤 輝郎
    1951 年 1 巻 2 号 p. 113-116
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
    Electrophoretic experiments of red blood cell hemolysates obtained by hemolysing with distilled water were carried out, in order to prove the allergic antibody, which has geen expected to adhere in the erythrocyte. Results obtained are as follows.
    1). The electrophoretic patterns were obtained on 9 rabbit, 28 human, and 2 goat red blood cell hemolysates from which stroma were removed. In these diagrams there were recorded a, c, Hb and b components mobilities of which were about -4.3, -1.9, and -1.1×10-5cm. 2 per volt per second at pH 8.0, respectively.
    2). b protein in rabbit and c protein in human red blood cell hemolysates were hardly demonstrable, but c protein in goat was remarkable.
    3). In rabbit red blood cell hemolysates, which were sensitized with the mixture of phosphatides of rabbit erythrocytes and ox sera or with ox sera only, the peak which was thought to be allergic antibody (X protein) was recorded as a component having mobility faster than Hb protein. The mobility of this pritein is about -2.9×10-5cm. 2 per volt per second. This peak disappeared at the 2nd day after reinjection of the antigen mentioned above.
    4). Some of human erythrocytes, in septicemia, subacute bacterial endocarditis and rheumatic diseases had X peak. In the bone marrows of these patients the erythropoesis was almost attacked. Not all the anemic diseases had X peak in toeir eryterocytes.
    5). c protein seems to be rich in lipid on account of its decrease of the area of electrophoretic pattern after extraction. It is interesting to find that in two cases of nephritic human cryrocytes, c component was remarkable.
    6). It is doubtful whether the peak appearing before or bshind Hb perk is due to the denaturing product either in vivo or in vitro, but it is more enhanced in rhcumatic diseases.
    In summer Hb peak were divided clearly into two peaks by denaturation when red blood cell hemolysates were kept at room temperature.
  • 菅野 浩
    1951 年 1 巻 2 号 p. 117-119
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
    1.10%食鹽水溶解,エーテル抽出後の全卵黄に對し0.05MNaHCO3-0.05MNa2CO3,PH10.0μ0.2のbufferを使用する事により全く沈澱を生ずる事なしに電氣泳動を實施し得た。
    2.その結果上昇下降兩圖形から少なくも8個の峰の存在を認め得たが成分の數は更に多い事が推定された。
    3.水溶性部分として少なくも3個の主な成分の存在する事,及び全卵黄に對するエーテル抽出操作がこれらの成分の相對濃度に與える影響が見られ,C.C.Shepard等(1949)と一致する結果が得られた。
    4.エーテル抽出4回の全卵黄及び非水溶性部分の泳動圖形が示され,全卵黄中水溶性成分の位置が推定きれた。
  • 四方 淳一
    1951 年 1 巻 2 号 p. 120-122
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
    Electrophoretic mobility of crystalline horse serum albumin was determined with Tiselius apparatus at various temperatures between 0°C and 30°C Electric charge per molecule of the protein was calculated by the application of moving boundaries. The results shows that electrophoretic mobility of the protein is approximately inversely inversely proportional to the viscosity of the solvent, while small deviations from this behavier can be interpreted as the displacement of electrolytic discossiation of the protein caused by the difference in temperature. This can also be proved by electric charge of the protein as calculated from electrophoretic mobility using the theory of moving boundaries.
  • 橋元 祐二, 永好 千鶴子, 緒方 昌一
    1951 年 1 巻 2 号 p. 123-126,112
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
    Electrophoretic analyses on blood plasma of children are so rare in our country that we studied the plasma protein analysis on 22 healthy children, 14 cases in 6 years (group A) and 8 yases in 2-4 years old (group B), with Tiselius' apparatus using Schlieren-diagonal method (Type HT-B Hitachi made).
    Pooled plasma were dialyzed for 16-20 hours, before electrophoresis, against 1/20M phosphate buffer.
    Experimental conditions were as follows; electric voltage 90-140V., electric current 9-10mA., time of electrophoresis 40-80 minutes, temperature at 16°C., Diagonal-Slit angle 25°.
    The results are shown in the next Table.
    In comparison with those values of the adult which were reported by many authors,
    1) total protein showed no remarkable difference,
    2) alubumin some decrease, and
    3) globulin no remarkable difference.
    3) globulin no remarkable difference.
    4) α-Globulin was not able to be compared with that of the adult, because there wag significant difference between group A and B. This may well be due to difference of time of electrophoresis.
    5) β-Globulin showed no remarkable differnce,
    6) γ-globulin some increase, and
    7) fibrinogen no remarkable difference.
  • 田中 一成, 財前 奉時, 林 靖
    1951 年 1 巻 2 号 p. 127
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
  • 1951 年 1 巻 2 号 p. 128-131,119
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
  • 1951 年 1 巻 2 号 p. 132-137
    発行日: 1951/06/15
    公開日: 2009/03/31
    ジャーナル フリー
feedback
Top