Basic conditions for the quantitation of serum protein fractions by thin layer gel filtration were investigated. The pattern of serum transferred on filter paper was recorded by an automatic recording densitometer and revealed 3 components (M, G, and A components) of serum protein corresponding to 19S, 7S and 4S by ultracentrifugation. Quantitative changes of the 3 components were characteristic in myeloma, macroglobulinemia, nephrosis and polyclonal hypergammaglobulinemia. The advantages of the thin layer gel filtration were not only its speed and its adaptability to very small amount of sample, but also its better separation than ultracentrifugation in certain molecular weight range.
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