Using the staining method of aldolase activity in disc electrophoresis gels
1), extracts of several higher plants were studied.
Spinach, radish and carrot seeds contained a single, aldolase fraction of almost the same mobility. Spinach leaves contained a single aldolase fraction, which migrated faster than that of the seed extract. Radish leaves contained two aldolase fractions, of which the faster moving fraction was more abundant. Radish and carrot roots contained two aldolase fractions. Therefore, it may be concluded that there are two aldolase isozymes in the higher plants, the faster migrating one is specific for leaves, the slower migrating one is specific for seeds. The former may be called
leaf aldolase and the latter
seed aldolase.
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