生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
15 巻, 2 号
選択された号の論文の5件中1~5を表示しています
  • 腎疾患患者の血清免疫グロブリン濃度と血清トランスフェリン濃度
    杉山 弘道, 黒羽 昭夫, 斉藤 秀晁
    1970 年 15 巻 2 号 p. 135-141
    発行日: 1970/08/30
    公開日: 2009/03/31
    ジャーナル フリー
    The levels in serum of transferrin and immunoglobulins were studied in the patients with renal diseases. The concentration in the sera were measured using a modification of the radial diffusion method of Mancini et al.
    The results were as follows:
    1) The serum transferrin concentration in the patients with subchronic nephritis, nephrotic syndrome, chronic nephritis and the patients under artificial dialysis was lower than normal.
    2) The serum IgG concentration in the patients with subchronic nephritis, chronic nephritis and the patients under artificial dialysis was lower, and the lowest in nephrotic syndrome.
    3) The serum IgA concentration in the patients under haemodialysis was normal. In the patients with subchronic nephritis and chronic nephritis, and in the patients under peritoneal dialysis, it was elevated. However, it was lowered in nephrotic syndrome.
    4) The serum IgM concentration in the patients with subchronic nephritis was higher, and that in the patients with other renal diseases were normal.
  • 小島 峯雄, 若原 達男, 青木 靖, 安藤 宏, 松友 啓典, 野手 信哉, 乾 成美
    1970 年 15 巻 2 号 p. 143-148
    発行日: 1970/08/30
    公開日: 2009/03/31
    ジャーナル フリー
    1) Fraction-I, which is a portion of serum prtoein separated by means of gel filtration using Sephadx G-200, formed a complex of trypsin-protein esterase (TPE) when treated with trypsin and did not lose its enzymatic activity. Mild anti-tryptic activity was also demonstrated in this fraction.
    The ratio of the tryptic activity to the anti-tryptic activity was 5. It is considered that the existence of anti-tryptic activity is due to a loss of enzymatic activity caused by the formation of the complex.
    2) The esterase activity of α2M-enzyme complex in serum was studied with the use of α-N-benzoyl-DL-arginine-p-nitroanilid HCl (BAPNA) as the synthesized substrate in human subjects. It was found that the serum protein, which had esterase activity, could be absorbed by anti-α2M rabbit serum immunochemically. This complex could also protect trypsin esterase activity from soybean trypsin inhibitor. Moreover, our data suggested that one mole of α2M was bound to two moles o trypsin.
    3) In 83 patients with various diseases, the values of serum TPE activity were compared with those of serum α2M measured by an immunological method. The result, that the correlation coefficient between the two values was 0.95 showed the greatly significant relation of TPE activity to α2M level in serum. Accordingly, the measurement of α2M concentration in serum can be replaced by the determination of serum TPE activity, which is a simpler technique.
  • 沢田 英夫, 矢田 昭一
    1970 年 15 巻 2 号 p. 149-153
    発行日: 1970/08/30
    公開日: 2009/03/31
    ジャーナル フリー
    A method for the purification of anti-H(O) agglutinin of eel serum utilizing preparative Disc electrophoresis apparatus was described. The purified hemagglutinin showed a single band on starch gel electrophoretic analysis. In the analytical ultracentrifuge, the agglutinin had an s020, w value of 6.2 S, and was found to be inhibited weakly by human group OSe saliva. The minimum agglutinating dose against human O group red cells was around 0.2μg/ml.
  • 濾紙電気泳動法による分解活性の簡易測定法と仔犬関節軟骨からの酵素抽出への応用
    塩野 正喜
    1970 年 15 巻 2 号 p. 155-163
    発行日: 1970/08/30
    公開日: 2009/03/31
    ジャーナル フリー
    The light fraction of protein-polysaccharide complex (PPL) was isolated from bovine nasal septum. The enzymic degradation of PPL by protease (trypsin) or polysaccharidase (testicular hyaluronidase (HAse)) was apparently demonstrated by paper electrophoresis (Beckman No. 320046 paper strips, length 7cm, pH, 8.6, 0.05M borate buffer, 60V, 30min), followed by toluidine blue staining. Under these conditions, PPL remaind at the starting line, while the protease-degraded polysaccharide chains migrated towards anode as a broad band. HAse digestion, however, yielded two or three bands which were different in shape and mobility from that of the protease digestion products. Applying the paper electrophoretic method for estimating the activities, the PPL-degrading enzymes were purified from puppy articular cartilage minces by water extraction, protamine treatment, Sephadex G-75 gel filtration, and electrofocusing (pH 3-10), successively. There were separated HAse- and protease-type enzymes with isoelectric points of 5.8 and 7.6, respectively. These enzymes exhibited the optimum pH at about 3-4. The present method was shown to be advantageous with respect to its simplicity, applicability to micro-quantity of the sample, and reliable discrimination between protease- and HAse-type enzyme activities.
  • 島尾 和男
    1970 年 15 巻 2 号 p. 165-169
    発行日: 1970/08/30
    公開日: 2009/03/31
    ジャーナル フリー
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