All issues
Successor
Volume 2 (1954 - 1955･･･
- Issue 4 Pages 247-
- Issue 3 Pages 167-
- Issue 2 Pages 105-
- Issue 1 Pages 3-
Volume 2, Issue 4
Displaying 1-6 of 6 articles from this issue
- |<
- <
- 1
- >
- >|
-
M. Takanami1955 Volume 2 Issue 4 Pages 247-258
Published: December 15, 1955
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESS -
Katsumi Takahashi1955 Volume 2 Issue 4 Pages 259-267
Published: December 15, 1955
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe author has studied the protein pattern of some experimental ascites induced by various methods by classical Tiselius procedure and paper electrophoresis and has obtained the following results.
1) Animals having an accumulation of ascitic fluid did not necessarily reveal hypoalbuminemia, but in general they showed higher percentage of albumin in ascitic fluid than in the peripheral blood.
2) Generally speaking, the protein patterns of concomittant ascitic fluids resembled that of serum regardless to the inducing procedures. Careful observation, however, indicates that in most cases, albumin percentage is higher in the ascites than in the circulating blood, but in some cases about equal or higher in the serum.View full abstractDownload PDF (5252K) -
Akira Inouye, Katsumi Takahashi1955 Volume 2 Issue 4 Pages 268-277
Published: December 15, 1955
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSIn this report the authors have intended to establish the theoretical and experimeatal basis of paper electrophoresis. Especially considerations on relative mobilities on paper were shown. We studied the effect of endosmosis and evaporation on the displacement of sample on paper. Furthermore, separating ability of paper electrophoretic apparatus was also referred to. The followings are concluded.
1. It was shown theoretically that principles of Kunkel and Tiselius and that of Macheboeuf et al. on mobility are applicable to the more generalised conditions (parallel change of electrophoretic and electroendosmotic force with uniform but changeable evaporation).
2. Evaporation effect demonstrated by Macheboeuff et al mainly depends upon Joule's heat due to electric current, and therefore, constant evaporation may be obtained in any kinds of apparatus.
3. Principles to determine relative mobility of each fraction of serum protein in paper electrophoresis and to identify unknown fractions, were presented.
4. Relative mobility of α2-glob. in paper electrophoresis is consistently lower than that obtained by Tiselius apparatus.View full abstractDownload PDF (4268K) -
I. On the interfacial potential of cytoplasmic granulesYutaka Tashiro1955 Volume 2 Issue 4 Pages 278-283
Published: December 15, 1955
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe interfacial potential of cytoplasmic granules, i. e., mitochondria, microsome and of ultra-microsome, was studied by means of classical moving boundary method and Tiselius' electrophoretic method, and the following results were obtained.
1. In Miller-Golder's buffer of Γ/2=0.1, the isoelectric-point was about pH 4.5.
2. In neutral or alkaline solution, these particles were negatively charged.
3. It seems to the author that owing mainly to this negative charge of the particles that cytoplasmic granules, such as microsome and ultramicrosome, can stably exist in cytoplasm, are basophilic, and do not agglutinate when the pH of mediumm is kept alkaline during cell fractionation.
4. Judging from the isoelectric points of granules, it may be supposed that there exists about equal amounts of lecithine and cephalin on the surface of granules, however, further experiments are necessary to determine this precisely.View full abstractDownload PDF (840K) -
I. Changes of Constituents in Prepartum and Postpartum Milk of Goats and Cows: -Especially on Milk ProteinsR. Yoh, K. Yamauchi1955 Volume 2 Issue 4 Pages 284-289
Published: December 15, 1955
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSInfluence of parturition upon the constituents of Saanen goat's and Holstein cow's milk was investigated in relation to the stage of prepartum and postpartum.
The results obtained here are briefly summarised as follows:-
The prepartum milk was almost resemble to colostrum in general. But in the case of goats it contained more milk-solids, protein and little less lactose than their colostrum. In the case of cow, however, prepartum milk contained less milk-solids, fat, and protein as compared with her colostrum. This difference between the dairy goats and cows may be due to the species specific lactating process.
Electrophoretic analysis demonstrated that the prepartum milk and colostrum of these two kinds of dairy animals contained much immune lactoglobulin as compared with their aged milk. In cow's milk the immune component rose from the seventh day before parturition toward the colostrum period, but in goats milk it declined from the third day before parturition toward the colostrum period, and falling rapidly two days after parturition. This component was almost precipitated with (NH4)2SO4 at 0.4 saturation.
Casein in prepartum milk or colostrum, especially in the former, was hardly precipitated with acid, but such remaining casein type protein was able to be precipitated entirely with rennet at pH 5.4.View full abstractDownload PDF (776K) -
II. Change of Blood Serum Proteins of Calf after Birth; -Especially on its Relationship to Colostrum Whey ProteinsR. Yoh, K. Yamauchi1955 Volume 2 Issue 4 Pages 290-294
Published: December 15, 1955
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSElectrophoretic observation was carried out on blood sera of three calves. Sera of new born calves contained no γ-globulin. It appeared after ingestion of colostrum, but its content was very low for two Holstein calves, it may be, as their initial suckling of colostrum was too late after birth (one day after birth). The serum of another calf (Guernsey) which received colostrum within 10 hours after birth contained it considerably.
This globulin which appeared in the serum of calf during ingestion period of colostrum fairly agreed with colostrum globulin electrophoretically. The slowest moving component, which is present in cow's serum, was apparently appreciable in the serum of calf after three months of birth, but it was still less than that of cow's serum. This component is probably no related to ingestion of colostrum.
Als in new born goat's serum, no γ-globulin was found. But appearance of this component was not successfully found within several hours after initial suckling.View full abstractDownload PDF (519K)
- |<
- <
- 1
- >
- >|