巻号一覧
後続誌
20 巻 (1976 - 1977)
- 4 号 p. 281-
- 3 号 p. 207-
- 2 号 p. 83-
- 1 号 p. 3-
20 巻, 1 号
選択された号の論文の6件中1～6を表示しています
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ピータース ヒューベルト1976 年 20 巻 1 号 p. 3-38
発行日: 1976/02/25
公開日: 2009/03/31
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三木 一正, 鈴木 宏, 飯野 四郎, 宮崎 純, 遠藤 康夫, 織田 敏次, 平野 和行, 杉浦 衛1976 年 20 巻 1 号 p. 39-44
発行日: 1976/02/25
公開日: 2009/03/31
ジャーナル フリーWe have reported that the electrophoretic mobility of human intestinal alkaline phosphatase (ALP) was different from that of adult intestinal ALP, and meconium ALP had the same enzymatic properties and electrophoretic mobilities as that of fetal intestinal ALP. In this report, using meconium ALP instead of fetal intestinal ALP, their properties were compared with that of the purified human intestinal ALP.
The results obtained were as follows:
1) The electrophoretic mobilities of meconium ALP were different from that of adult intestinal ALP.
2) The electrophoretic mobilities of a new-born baby's fecal ALP moved gradually to the cathode after birth. On the 5th day after birth, its mobility became similar to that of adult intestinal ALP.
3) On enzymatic properties, such as heat-stability, Km value and effects of many inhibitors, meconium ALP and adult intestinal ALP were similar.
4) By double immunodiffusion and electrophoresis with human anti-intestinal ALP antibody, they showed identical reaction.
5) By neuraminidase treatment, meconium ALP migrated to the cathode and the same position as adult intestinal ALP which showed no migration by this treatment.
6) These results showed that fetal intestinal ALP is not the new ALP isoenzyme but is the modified enzyme of adult intestinal ALP. The difference in their electrophoretic mobilities is probably determined by the amount of sialic acids contained in this enzyme.抄録全体を表示PDF形式でダウンロード (4032K) -
益沢 学, 鎌田 武信, 佐子山 豈彦, 荻田 善一, 伊藤 文雄1976 年 20 巻 1 号 p. 45-48
発行日: 1976/02/25
公開日: 2009/03/31
ジャーナル フリーWe have encountered a patient with gastric carcinoma metastatic to the liver, who had heat stable alkaline phosphatase in his serum and body fluid. The properties of his carcinoplacental alkaline phosphatase were studied and found almost identical to Nagao isoenzyme.
However, this carcinoplacental alkaline phosphatase showed a broad isozyme band from the position of variant alkaline phosphatase to placental alkaline phosphatase on a thin layer polyacrylamide gel electrophoresis of 5-7% gel concentration. It was separated into two parts, fast and slow, from a 6% polyacrylamide gel slab and the properties of them were studied.
The data obtained was almost identical to each other, although some microheterogeneities existed. The isozyme bands of this carcinoplacental alkaline phosphatase were also electrophoresed as the fastest migrating isozyme band, when polyacrylamide gel of over than 8% concentration was used.
It is suggested that the epigenetic modification may cause microheterogeneities of this isozyme except for the effect of genetic expression of molecular structure.抄録全体を表示PDF形式でダウンロード (1621K) -
古賀 俊逸, 平山 千里, 井林 博1976 年 20 巻 1 号 p. 49-52
発行日: 1976/02/25
公開日: 2009/03/31
ジャーナル フリーAn immunochemical method for determining the concentration of plasma β-lipoprotein was developed. The assay was carried out by electrophoresis in an antibody-containing agarose gel (rocket immunoelectrophoresis or quantitative immunoelectrophoresis).
The antiserum was prepared in a rabbit injecting low-density lipoprotein (d. 1.019-1.063) which had been isolated by ultracentrifugation. 10ml of 1% agarose gel containing 2% (v/v) antiserum was spilled over a 84×94mm glass plate and gelled. On each plate 15 holes, 2.5mm in diameter, were punched out on its cathodal side. An aliquot of serum was incubated with an equal amount of 2M KCNO at 50°C for 20 minutes so the proteins were carbamylated. After adequate dilution, 4μl of the sample was applied to the hole. Four holes out of fifteen. on each plate were preserved for reference serum which was obtained from a healthy male person at the age of 39 years and processed equally as the sample serum. The concentration of β-lipoprotein in the reference serum was taken arbitrarily as 100 units/100ml. The electrophoresis was carried out with constant voltage at 2V/cm for 16 hours at 15°C. The height of the precipitin line was measured after staining with amidoblack 10B.
The average β-lipoprotein concentration for 20 normal subjects at the age of 18-22 years was 96±18 (mean±sd) units/100ml. Levels of β-lipoprotein for the subjects at the age of 36-65 years were 124±27 for 18 normal controls, 151±31 for 12 patients with an inactive form of chronic hepatitis 143±41 for 18 patients with an active form of chronic hepatitis, 113±37 for 33 cirrhotic patients and 187±61 for 22 subjects with cholestasis.
The reproducibility of the measurement was satisfactory. This method described here measures total apoprotein B and seems more sensitive than single radial immunodiffusion and easier to perform than radioimmunoassay.抄録全体を表示PDF形式でダウンロード (1682K) -
脳血管障害について河北 英詮, 山内 紀之, 蓮江 郁夫1976 年 20 巻 1 号 p. 53-60
発行日: 1976/02/25
公開日: 2009/03/31
ジャーナル フリーDisc electrophoresis on polyacrylamide gel was carried out on cerebrospinal fluid (CSF) protein of 146 cases with cerebral vascular diseases.
1) The electrophoretic patterns of CSF protein are usually classified into 10 types. The frequencies of appearance of each type in our case are given in parenthesis. Mixed type (12%), albumin type (12%), MII+AII type (8%), γ-globulin type (16%), prealbumin increased type (1%), prealbumin decreased type (8%), prealbumin 3 fraction type (0%), concentration type (2%), combination type (13%), and normal type (28%).
2) Abnormal electrophoretic patterns of CSF protein were observed in 43 out of 62 cases with cerebral aneurysm (mean total protein in CSF is increased: 47.3mg/dl), 14 out of 19 with subarachnoidal bleeding (increased: 82.6mg/dl), 16 out of 19 with cerebral hemorrhage (increased: 48.6mg/dl), 8 out of 10 with intracranial bleeding (increased: 50.6mg/dl), 13 out of 18 with cerebral softening (increased: 46.7mg/dl), 2 out of 4 with subdural bleeding (not increased: 33.3mg/dl), and 5 out of 14 with other cerebral vascular diseases (not increased: 31.5mg/dl).抄録全体を表示PDF形式でダウンロード (7998K) -
島尾 和男1976 年 20 巻 1 号 p. 65-66
発行日: 1976/02/25
公開日: 2009/03/31
ジャーナル フリーPDF形式でダウンロード (272K)
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