SEIBUTSU BUTSURI KAGAKU

Cellulose acetate electrophoresis using a new Separax-S membrane and its normal serum protein fractional values in adults and children

Tadashi Kawai, Yasuko Yamagishi, Tsuneko Kawai

1977 Volume 20 Issue 4 Pages 281-286
Published: February 25, 1977
Released on J-STAGE: March 31, 2009

DOIhttps://doi.org/10.2198/sbk.20.281

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An improved new membrane, Separax-S, was studied for its routine application of the serum protein fractionation. In comparison to the conventional Separax membrane, Separax-S has less endoosmotic effect and gives more reproducible and clear protein sepration. The application point of the serum now locates on the beta fraction, and the linear beta-lipoprotein band is less apparent. The fractional values of the serum protein are not significantly different from those of the conventional membrane, except that the beta fraction is slightly higher with the new membrane, Separax-S. Glycoproteins, lipoproteins, and isoenzymes of lactate dehyrogenase and alkaline phosphatase could be separated sufficiently with the new membrane.
The normal serum protein fractional values in both adults and children are reported by using the new Separax-S membrane with the standard methodology.

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Studies on the glycopeptide in digestive tracts

I. The distribution in stomachs of different animal species

Hiroshi Masuda, Shigeki Shichijo, Mutsuya Takeuchi

1977 Volume 20 Issue 4 Pages 287-291
Published: February 25, 1977
Released on J-STAGE: March 31, 2009

DOIhttps://doi.org/10.2198/sbk.20.287

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Glycopeptides were isolated from tryptic digests of eleven mammalian species stomachs, including human, rabbit, horse, cow, pig, goat, sheep, dog, cat, guinea pig and rat.
The results have been dsicussed and summarized as follows.
1. The glycopeptides content was higher in the mucosa than in the muscularis-serosa.
2. The sialic acid- and sulfate-rich glycopeptide, which migrated toward the anode faster and stained with both alcian blue and PAS, was absent or sparingly present in the mucosa of the human, rabbit, horse and first to third stomachs of the cow, goat and sheep, whereas in the mucosa of the pig, dog, cat, guinea pig, rat and the fourth stomach of the cow, goat and sheep they were found in noticeable extents.

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Studies on the glycopeptide in digestive tracts

II. The distribution in different parts of digestive tract

Hiroshi Masuda, Shigeki Shichijo, Mutsuya Takeuchi

1977 Volume 20 Issue 4 Pages 293-297
Published: February 25, 1977
Released on J-STAGE: March 31, 2009

DOIhttps://doi.org/10.2198/sbk.20.293

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Glycopeptide was isolated from tryptic digests of different parts of digestive tract, including esophagus, small intestine, large intestine and rectum of man and animals. Analysis was carried out by cellulose acetate strip electrophoresis, followed by staining with PAS (Schiff) and alcian blue.
The results have been discussed and summarized as follows.
1. The glycopeptide content was higher in the mucosa than in the muscular-serosa and was highest in the rectum mucosa, while the glycosaminoglycan indicated almost the same amount throughout the digestive tract.
2. The glycopeptide which stained with both alcian blue and PAS was absent or sparingly present in the mucosa of the small intestine, whereas it was found in noticeable extents in the mucosae of the esophagus, large intestine and rectum.

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Proceedings of the 26th Spring Meeting of the Society of Electrophoresis

1977 Volume 20 Issue 4 Pages 299-342
Published: February 25, 1977
Released on J-STAGE: March 31, 2009

DOIhttps://doi.org/10.2198/sbk.20.299

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