生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
21 巻, 2 号
選択された号の論文の3件中1~3を表示しています
  • 村山 和代, 菅野 剛史
    1977 年 21 巻 2 号 p. 57-60
    発行日: 1977/08/25
    公開日: 2009/03/31
    ジャーナル フリー
    A sensitive and separative fluorometric analysis of alkaline phosphatase isoenzyme was described. Electrophoretical separation of alkaline phosphatase isoenzyme was based on acetate gel (Cellogel) electrophoresis with the discontinuous buffer system, and the locarization of alkaline phosphatase isoenzyme was fluorometrically visualized with naphthol ASMX-phosphate as substrate. The liberated naphthol ASMX which has a strong fluorescence at 505nm on the acetate gel were scanned by scanning fluorophotometer, and the peak areas obtained from scannings were proportional to the activities of each separated isoenzyme. The standard curves were linear in the rang of 5 to 640 U/1. The coefficient of variation of this method as a quantitative analysis of enzyme separated was 3 to 5 per cent in the same membrane and 8 to 10 per cent in the different membranes.
    Using this method, the kinetical inhibition studies of alkaline phosphatase isoenzymes were easilly carried out by the substrate solution containing different concentration of inhibitors, and the kinetical values of the enzymes in the solution of enzyme mixture were clearly coincided with the kinetical values obtained from the purified enzymes. The usefullness and application of this sensitive microtechniques in clinical examination was studied and discussed.
  • 益沢 学
    1977 年 21 巻 2 号 p. 61-66
    発行日: 1977/08/25
    公開日: 2009/03/31
    ジャーナル フリー
    Carcinoplacental alkaline phosphatase (CPALP), including three subtypes, such as Regan, Nagao and variant, has been found mainly by electrophoretic procedures. Using polyacrylamide gel slab of 5% (w/v) concentration, CPALP (Regan, Nagao) and placental alkaline phosphatase are electrophoresed next to the place of bone type isozyme or at the same place of it, and must needs to be differentiated by other methods, such as heat treatment.
    Using polyacrylamide gel electrophoresis, CPALP was found as the fastest migrated isozyme band to the anode, when the polyacrylamide gel concentration of above than 8% is used. Thus, a polyacrylamide gradient gel electrophoresis method was introduced. Using this modified method of 16-8% gel concentration, CPALP could be detected as the fastest migrated isozyme band to the anode, and subbands of CPALP could also be identified.
  • 1977 年 21 巻 2 号 p. 89-141
    発行日: 1977/08/25
    公開日: 2009/03/31
    ジャーナル フリー
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