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Volume 21 (1977 - 197･･･
- Issue 4 Pages 249-
- Issue 3 Pages 151-
- Issue 2 Pages 57-
- Issue 1 Pages 15-
Volume 21, Issue 3
Displaying 1-14 of 14 articles from this issue
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Hiroshi Sugano1977 Volume 21 Issue 3 Pages 151-158
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
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Tadashi Kawai1977 Volume 21 Issue 3 Pages 159-167
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
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Motoshi Kitamura1977 Volume 21 Issue 3 Pages 169-170
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
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Keiichi Omoto1977 Volume 21 Issue 3 Pages 171-176
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
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Toshimasa Nakayama, Motoshi Kitamura1977 Volume 21 Issue 3 Pages 177-180
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
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Mieko Saga1977 Volume 21 Issue 3 Pages 181-185
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
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Fumiko Tanaka, Nobuyuki Amino, Chozo Hayashi, Kiyoshi Miyai, Yuichi Ku ...1977 Volume 21 Issue 3 Pages 187-191
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
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Mitsutaka Nagamine1977 Volume 21 Issue 3 Pages 193-199
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
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Shojiro Kano1977 Volume 21 Issue 3 Pages 201-208
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
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Katsuhiko Kuwa, Toshimasa Nakayama, Motoshi Kitamura1977 Volume 21 Issue 3 Pages 209-214
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
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Hiroshi Masuda, Shigeki Shichijo, Mutsuya Takeuchi1977 Volume 21 Issue 3 Pages 215-220
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSGlycosaminoglycans were isolated from the human gastric mucosa. Pevikon-C 870 block electrophoresis and Dowex 1×2 column chromatography were used.
Fractionation of nondialyzable material by Dowex 1×2 column chromatography yielded six fractions at the molarity of NaCl ranging from 0.5 to 2.0 mole; they were identified as hyaluronic acid, heparan sulfate, chondroitin sulfate -A, -B and -C, respectively.View full abstractDownload PDF (3641K) -
III. Isolation and characterization of the glycopeptide from gastric mucosaHiroshi Masuda, Shigeki Shichijo, Mutsuya Takeuchi1977 Volume 21 Issue 3 Pages 221-226
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSGlycopeptides were isolated from tryptic digests of the human, bovine 4th and swine gastric mucosa. The separation of the glycopeptides was performed by preparative zone electrophoresis in a Pevikon C-870 block. After gel filtration of certain fractions on Biogel A-50m, final separation was achieved by chromatography on DEAE cellulose. The results have been discussed and summarized as follows.
Fractionation of nondialyzable glycopeptide from human stomach tissue by DEAE cellulose column chromatography yielded two fractions and from both bovine 4th and swine stomach tissues five fractions, respectively; they were generally characterized by an increase in sialic acid and sulfate as the molarity of NaCl solution increased. Hexose was the main component and hexosamine was the next in all fractions from all materials.View full abstractDownload PDF (662K) -
I. Basic examination of the method and protein fractions of normal and diseased urineKiyoko Sano, Kiyoko Kanamori, Junko Manaka, Hiroko Cho, Noriko Hasunum ...1977 Volume 21 Issue 3 Pages 227-232
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSSodium dodecyl sulfate polyacrylamide gel electrophoresis was applied to fractionation of urine protein. Basic examininations on pretreatment of urine sample, gel concentration, reducing process and electrophoresis were carried out and their optimal conditions were described.
By this method, 8 bands were detected in normal urine and 21 bands in abnormal urine. The molecular weight of these proteins was presumed to range from 250, 000 to 10, 000.View full abstractDownload PDF (5412K) -
Seijin Hosaki, Kiyoko Sano, Hiroko Cho, Junko Manaka1977 Volume 21 Issue 3 Pages 233-239
Published: November 30, 1977
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSHeterogeneity of serum γ-glutamyltranspeptidase (GTP) of 23 normal subjects and 82 patients with hepatobiliary diseases were studied by electrophoresis on “Cellogel”.
Nine fractions in total were detected in normal and diseased groups. Enzymograms of normal controls were classified into the following three types; 1) a single broad band between albumin and α2 globulin (52.2%), 2) a single sharp band at α1-α2 globulin (39.1%), 3) double bands at albumin-α1 globulin and α1-α2 globulin (8.7%).
Enzymograms of patients with hepatobiliary diseases were different from those of normal control.
The fractions were classified to main and sub fraction according to density of bands and frequency of appearance of individual fraction as main or sub fraction in hepatobiliary diseases was investigated.
Characteristic features of enzymograms in various hepatobiliary diseases were demonstrated and clinical significance of several fractions which appeared frequently were discussed.View full abstractDownload PDF (4795K)
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