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Volume 22 (1978 - 197･･･
- Issue 4 Pages 261-
- Issue 3 Pages 185-
- Issue 2 Pages 117-
- Issue 1 Pages 1-
Volume 22, Issue 2
Displaying 1-11 of 11 articles from this issue
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Koichi Anan, Kazunori Takeo1978 Volume 22 Issue 2 Pages 117
Published: October 30, 1978
Released on J-STAGE: March 31, 2009
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Koichi Anan1978 Volume 22 Issue 2 Pages 119-122
Published: October 30, 1978
Released on J-STAGE: March 31, 2009
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Kazuo Murakami, Fumiaki Suzuki, Hideyuki Kobayashi1978 Volume 22 Issue 2 Pages 123-128
Published: October 30, 1978
Released on J-STAGE: March 31, 2009
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Kunihiko Ishitani1978 Volume 22 Issue 2 Pages 129-134
Published: October 30, 1978
Released on J-STAGE: March 31, 2009
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Tsutomu Inoue1978 Volume 22 Issue 2 Pages 135-138
Published: October 30, 1978
Released on J-STAGE: March 31, 2009
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Kazunori Takeo, Masanori Fujimoto, Ryosuke Suzuno, Akira Kuwahara1978 Volume 22 Issue 2 Pages 139-144
Published: October 30, 1978
Released on J-STAGE: March 31, 2009
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Hiroshi Masuda, Shigeki Shichijo, Tomoyoshi Goya, Mutsuya Takeuchi1978 Volume 22 Issue 2 Pages 145-149
Published: October 30, 1978
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSGlycopeptide was isolated from tryptic digest of the liver of rats fed with hepatic carcinogen, 3'-methyl-4-dimethylaminoazobenzene (3'-methyl-DAB), for 3-35 weeks. Analyses were carried out by the measurements of hexose, sialic acid and hexosamine as well as cellulose acetate strip electrophoresis, followed by staining with alcian blue.
The results have been discussed and summarized as follows.
1. The hexose, sialic acid and hexosamine contents all rose in the first 5-7 weeks of feeding with 3'-methyl-DAB, falling once to a low level and rose again after 24 weeks of feeding with hepatic carcinogen, especially in the cancer tissue during 24-35 weeks.
2. The relative proportion of the glycopeptide fraction on cellulose acetate strip electrophoresis also showed similar alterations with weeks of feeding with azo dye as observed before.View full abstractDownload PDF (702K) -
Osamu Sugita, Minoru Yakata1978 Volume 22 Issue 2 Pages 151-156
Published: October 30, 1978
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSIn this report, the immunochemical properties of LDH-bound immunoglobulins in nine cases (five cases bore the complexes with IgA (K), two with IgG (L), one with IgG (K) obtained from pleural effusion ano one with IgA-IgG mixture) are described.
Specific binding to LDH-2 and LDH-3 was found in the case of IgA (K) type and that to LDH-4 and LDH-5 in IgG (L) type.
Furthermore, LDH-1, LDH-4 and LDH-5 were found in IgG (K) pleural effusion case and all LDH isozymes in IgA-IgG mixture case.
Immunoglobulins were separated from each insolubilized LDH isozyme using sepharose 4B activated by cyanogen bromide.
The immunoglobulin obtained from insolubilized LDH-2 reacted with both LDH-2 and LDH-3 by thin layer gel filtration. Likewise, that from insolubilized LDH-5 was found to react with both LDH-1 and LDH-5.
Therefore, in the present cases, it may be considered that there are a common antigenetic site in LDH-2 and LDH-3 molecules and also another common antigenetic site in LDH-1 and LDH-5.View full abstractDownload PDF (4060K) -
Iichiro Kondo, Teruo Kodaira, Minoru Matsuzaki, Yoshiro Shioda, Teiji ...1978 Volume 22 Issue 2 Pages 157-163
Published: October 30, 1978
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSSerum proteinase inhibitors such as C1S-inhibitor (C1S-INH), α1-antitrypsin (α1-AT), antithrombin III (AT-III) and α2-macroglobulin (α2-M) were measured in 156 patients with malignant tumors of various organs.
Results;
1. Generalized findings in all patients examined: C1S-INH and α1-AT increased and AT-III decreased as the stage of tumors progressed. A significant increase of α2-M was found exclusively in the male patients, but changes became unremarkable following the progression of illness.
2. Changes in each patient group with tumors of different organs: (a) α2-M significantly increased also in the female patients in advanced stage of cancer of the lungs. (b) AT-III rather increased in cancer of the cervix uteri. (c) α2-M significantly increased even in earlier stage of cancer of the urinary bladder. (d) AT-III remarkably decreased in cancer of the prostate.
These changes of serum proteinase inhibitors are assumed to contribute beneficial environment for the growth of tumors.View full abstractDownload PDF (707K) -
Akio Kiuchi1978 Volume 22 Issue 2 Pages 165-169
Published: October 30, 1978
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSBovine fetal serum was investigated by cellulose acetate electrophoresis, immunoelectrophoresis, immunodiffusion and single radial immunodiffusion.
A continuous increase of the total serum proteins was observed, it reached 4.2g/dl at birth.
Significant changes of serum proteins were observed, especially α-glob, by cellulose acetate electrophoresis in the course of gestation age.
Twelve precipitation lines were always observed in the fetal serum by immunoelectrophoresis, seven of 12 lines were identified.
Serum transferrin and α2-macroglobulin were also studied.
IgG was detected from 6 (16%) of 37 fetal sera. IgG1 was the predominant immunoglobulin in the sera of IgG detected samples.View full abstractDownload PDF (1465K) -
Takashi Manabe, Kumiko Tachi, Kiyotsugu Kojima, Tsuneo Okuyama1978 Volume 22 Issue 2 Pages 171-177
Published: October 30, 1978
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA technique of two-dimensional polyacrylamide gel electrophoresis for the separation of plasma proteins was described in detail. Human plasma proteins were separated by isoelectric focusing followed by electrophoresis in a 4 to 21% or a 4 to 30% linear gradient slab gel. Urea or SDS was not used throughout the procedure, so that the analyses of proteins in their biologically active state were possible. By this technique, human plasma protein was resolved into more than 250 spots.View full abstractDownload PDF (6504K)
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