生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
23 巻, 3 号
選択された号の論文の8件中1~8を表示しています
  • 1.分子量とサブユニット構成
    小林 邦彦, 平井 秀松
    1979 年 23 巻 3 号 p. 183-189
    発行日: 1979/12/10
    公開日: 2009/03/31
    ジャーナル フリー
    Chicken polymeric immunoglobulin of IgM from serum, and IgA from bile and intestinal contents were purified and characterized for their subunit compositions.
    It was found that two IgM with different molecular weight of 860, 000 and 680, 000 daltons were present in chicken serum and that these two IgM were considered to be pentameric and tetrameric IgM, respectively. Both IgA from bile and intestinal contents showed the same molecular weight of 560, 000 daltons and were estimated to have trimeric structure.
    Presence of J-chain was confirmed only in polymeric immunoglobulins of IgM and IgA but not in IgG by alkaline-urea-polyacrylamide gel electrophoresis. One J-chain was found per mole of polymeric immunoglobulin by subunit analyses of chicken biliary IgA.
    Neither additional antigenic determinants nor a special protein moiety due to a putative chicken secretory components (SC) could be verified in IgA from various secretions by both immunological and sodium dodecyl sulfate polyacrylamide gel electrophoretic analyses.
    Two light-chains with different molecular weight were found by sodium dodecyl sulfate acrylamide gel electrophoresis of chicken IgG, IgA and IgM. These two light-chains were assumed to be corresponded to two isotypes of chicken light-chains of IgY (G), reported recently.15)
  • 等電点電気泳動と酵素活性について
    飯島 武, 門福 強樹, 佐藤 永雄
    1979 年 23 巻 3 号 p. 191-195
    発行日: 1979/12/10
    公開日: 2009/03/31
    ジャーナル フリー
    Rat liver arginase activity was decreased during the development of hepatoma by p-dimethylaminoazobenzene (DAB) feeding. The most decreased activity was observed at 30 days after initiation, but the enzyme at 120 days showed nor marked difference from that of normal rat. The arginase activity of DAB-induced hepatoma diminished about to one-sixth of the normal level.
    It was shown that DAB affected the isoelectric point of rat liver arginase; that is, pH 9.3 of the isoelectric point of arginase from normal liver was altered to 9.1 in the liver of rat fed of DAB for 90 days. Arginase from DAB-induced hepatoma and fetal rat liver showed the same isoelectric point hepatocarcinogen in rat liver like other enzymes.
    Rat liver arginase was influenced by ornithine and carbamoyl phosphate. The enzyme activity was depressed by addition of ornithine, but activated in the presence of carbamoyl phosphate.
  • 七條 茂樹, 増田 博, 佐々木 洋二, 武内 睦哉
    1979 年 23 巻 3 号 p. 197-202
    発行日: 1979/12/10
    公開日: 2009/03/31
    ジャーナル フリー
    A study has been made of glycosaminoglycans in the several regions of the human heart including the right ventricular wall, pulmonary valve, interventricular wall, left ventricular wall and aortic valve, and also pulmonary artery and aorta. The uronic acid content in the aortic valve was approximately 8 times more than that in the ventricular walls.
    Cellulose acetate strip-electrophoresis of concentrates from the ventricular walls, pulmonary artery and aorta in barbital buffer revealed the presence of three alcian blue positive fractions, numbered in order of increasing electrophoretic mobility, fraction I, fraction II and fraction III, respectively; the fraction I corresponded in mobility to hyaluronic acid, fraction II to heparan sulfate, and fraction III to chondroitin-4-sulfate, dermatan sulfate and chondroitin-6-sulfate, whereas in both the aortic valve and pulmonary valve, the fraction II which corresponded with the mobility of heparan sulfate was scarecely found.
  • 松橋 直
    1979 年 23 巻 3 号 p. 203
    発行日: 1979/12/10
    公開日: 2009/03/31
    ジャーナル フリー
  • 島尾 和男
    1979 年 23 巻 3 号 p. 205-208
    発行日: 1979/12/10
    公開日: 2009/03/31
    ジャーナル フリー
  • 橋本 信也
    1979 年 23 巻 3 号 p. 209-217
    発行日: 1979/12/10
    公開日: 2009/03/31
    ジャーナル フリー
  • 菅野 剛史
    1979 年 23 巻 3 号 p. 219-222
    発行日: 1979/12/10
    公開日: 2009/03/31
    ジャーナル フリー
  • 1979 年 23 巻 3 号 p. 223-289
    発行日: 1979/12/10
    公開日: 2009/03/31
    ジャーナル フリー
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