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Volume 24 (1980 - 198･･･
- Issue 4 Pages 289-
- Issue 3 Pages 203-
- Issue 2 Pages 115-
- Issue 1 Pages 19-
Volume 24, Issue 4
Displaying 1-11 of 11 articles from this issue
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Sumiko Hashimoto1981 Volume 24 Issue 4 Pages 289-293
Published: 1981
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSEighty cases of cryoglobulinemia were studied on its components and biological activities, and following conclusions were obtained.
(1) Eighty cases of cryoglobulinemia were classified into following three types. Type I cryoglobulin which was composed of simple monoclonal immunoglobulin (8 cases). Type II cryoglobulin which was mixed cryoglobulin with a monoclonal component and polyclonal immunoglobulins (19 cases). Type III cryoglobulin which was mixed cryoglobulins without detection of monoclonal component (53 cases).
(2) The cryoprecipitate was found the largest amount in type I, and the least amount in type III.
(3) In the 63.1% (12/19) of type II cryoglobulin, M-protein was detected only in the purified cryoprecipitate, but that was not demonstrated in the original serum by immunoelectrophoresis.
(4) It was suggested that type III cryoglobulin may also contain M-component which could not detected because of the small quantities of cryoprecipitates.
(5) RF activity was detected in mixed type cryoglobulins (type II, III) at high frequency. But there was no clear differences in the occurrence of ANF activity between the cryoglobulin types.View full abstractDownload PDF (1992K) -
Ichiyo Tei, Yoshiaki Makino, Ikue Kanamaru, Kunio Konno1981 Volume 24 Issue 4 Pages 295-300
Published: 1981
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSHuman transferrin was purified from serum using polyacrylamide gel disc electrophoresis. The purified transferrin appeared to be free from other protein, as judged by electrophoresis on polyacrylamide gel and by immunoelectrophoresis. The purified human transferrin was resolved into a faster moving band (TF) and a slower moving band (TS) by 5.25% polyacrylamide gel electrophoresis. Analysis of the purified human transferrin by isoelectric focusing column separated this iron-transport protein into three components with isoelectric points of 5.61, 5.18 and 4.53. Electrophoresis on 5.25% polyacrylamide gel was carried out on each fraction from the column. Fractions pI 5.61 and pI 5.18 both moved with the same mobility as the slower electrophoretic component (TS). Fraction pI 4.53 moved with the same mobility as the faster electrophoretic component (TF). After recovery from the isoelectric column pI 5.18 transferrin was found to contain 0.90, 1.57 atoms of iron/mol of protein. The pI 4.53 transferrin had less than 0.05 atoms of iron/mol of protein. The pI 5.61 transferrin contained 0.51, 0.65 atoms of iron/mol of protein.View full abstractDownload PDF (6996K) -
Fusae Kanemitsu, Isami Kawanishi, Jun Mizushima1981 Volume 24 Issue 4 Pages 301-308
Published: 1981
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSIsoenzymes of creatine kinase (CK) with irregular electrophoretic mobility were found in sera from 5 patients. Enzyme immunoelectrophoresis and electroimmunofixation revealed that these unusual isoenzymes were complexes of CK and IgA. Light chain of CK linked IgA was lambda type.
The native CK isoenzymes in the complexes were identified as CK-MM, since apparent Michaelis constant values of the complexes were similar to that of CK-MM. It is interesting that all donor patients were elder and had malignant tumors near the terminal stage.View full abstractDownload PDF (3309K) -
A patient serum of expect in autoimmuno diseaseTakayoshi Matsuo, Yoshihiro Motomiya, Eigoro Okajima, Seiko Tatsumi, Y ...1981 Volume 24 Issue 4 Pages 309-312
Published: 1981
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (469K) -
Toshiho Nishita, Kyoichi Tanaka1981 Volume 24 Issue 4 Pages 313-318
Published: 1981
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSBy using the simple thin layer-PAGE, lactate dehydrogenase (LDH) isozymes in bovine serum were determined during the development of fetal and normal pregnant stage, and the isozymes in serum from patient with downer cow syndrom were determined.
All five forms of LDH isozymes were obserbed in bovine fetal serum. The total LDH activity and the fast-moving isozyme LDH1 increased in bovine fetal serum during development.
In the pregnant maternal serum, LDH1, LDH2 and LDH3 were detected, while LDH4 and LDH5 were not detected, in which LDH1 had the greatest percentage. There were no changes of LDH isozyme pattern in bovine serum during the pregnancy.
The slow-moving isozyme LDH5 and total LDH activities most increased in serum from patients with downer cow syndrom.View full abstractDownload PDF (1392K) -
Tsuyoki Kadofuku, Takashi Manabe, Tsuneo Okuyama1981 Volume 24 Issue 4 Pages 319-325
Published: 1981
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA detection method of α-amylase activity after two-dimensional electrophoresis was described. An agar-containing filter paper was overlaid on a slice (2mm in thickness) of a slab gel after two-dimensional electrophoresis. Then a 1mm thick blue starch polymer-containing Sepharose layer was overlaid on the ager-filter paper and kept at 37°C. As α-amylase was detected on the filter paper, the filter paper was dried up and densitometry was easy performed. Further, the stained spots on the filter paper did not decolorize after 6 months' preservation.
Human body fluid samples (5 sera, 2 salivas, 1 pancreatic juice, and 1 urine) were subjected to the activity staining method. Two to six activity spots of α-amylase were detected. The spots were classified into four main species by their isoelectric points; pI 6.9, pI 6.5, pI 5.9, and pI 5.3. The molecular weights of the spots ranged from 140, 000 to 500, 000.View full abstractDownload PDF (5373K) -
Production of α1-microglobulin by a hepatoma cell line(C-Hc4)Hiromitsu Enomoto, Yoshihisa Itoh, Kimiteru Takagi, Hirotomo Harada, T ...1981 Volume 24 Issue 4 Pages 327-333
Published: 1981
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSα1-microglobulin(α1-m)was detected by radioimmunoassay in the culture medium of a hepatoma cell line (C-Hc4). The level of this protein in the culture medium measured by radioimmunoassay increased proportionately with cell numbers. With the addition of puromycin and cycloheximide to the culture medium, the production of this protein was completely blocked. It was, therefore, suggested that this protein was actively produced by hepatoma cells.
This protein showed a complete immunochemical identity with purified α1-m, serum and urinary α1-m from a patient with renal insufficiency by Ouchterlony immunodiffusion and tandem crossed immunoelectrophoresis.
In crossed immunoelectrophoresis, this protein migrated at the α1-region, being slightly slower than purified and serum α1-m. On SDS disc electrophoresis combined with immune precipitation, this protein was also found to migrate slightly slower than purified α1-m. These results might be caused by a minor difference of its constituent from that of the purifiedα 1-m during the process of synthesizing this protein in hepatoma cells.View full abstractDownload PDF (4051K) -
Kojiro Matsumoto, Akira Sonoda, Shojiro Kano1981 Volume 24 Issue 4 Pages 335-340
Published: 1981
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSSeparation of immunoglobulin-linked LDH in patient serum was performed by high performance liquid chromatography and post-column enzyme reactor.
In the case of high speed anion exchange chromatography with IEX-525-QAE (4.0 i. d.×50mm), abnormal chromatograms of IgA-linked LDH and IgG-linked LDH were obtained. Minor difference in separation mechanisms were observed between electrophoretic and anion exchange chromatographic methods.
In the case of high speed gel permeation chromatography with TSK-5, 000 PW (7.6 i. d.×600mm) and double TSK-3, 000 SW (7.6 i. d.×600mm), IgA-linked LDH was completely separated from normal size of LDH and determination of each LDH activity was performed. Moreover, IgG-linked LDH was separated to two high molecular weight of LDH. This finding will suggest that IgG-linked LDH will be different in its binding forms from IgA-linked LDH.View full abstractDownload PDF (690K) -
Yuji Sugita, Takuji Takeuchi, Tokuhiko Higashi1981 Volume 24 Issue 4 Pages 341-346
Published: 1981
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSTyrosinase was purified from B16 mouse melanoma cells. Anti-tyrosinase antiserum was prepared using rabbits. Polysomes were prepared from the mouse melanoma cells and were found to be active in in vitro protein synthesis. The tyrosinase-synthesizing Polysomes were isolated from both free and membrane-bound Polysomes by the immunoprecipitation method. Results of our study indicate that both free and membrane-bound polysomes are responsible for synthesizing tyrosinase.View full abstractDownload PDF (2374K) -
Theoretical Proof of the Law of pH MonotonyKazuo Shimao1981 Volume 24 Issue 4 Pages 347-352
Published: 1981
Released on J-STAGE: March 31, 2009
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Katsuaki Miyatani1981 Volume 24 Issue 4 Pages 353-355
Published: 1981
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (1122K)
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