生物物理化学

電気泳動学会30周年記念総会に当って

平井秀松

1980 年 24 巻 2 号 p. 115-120
発行日: 1980/09/25
公開日: 2009/03/31

DOIhttps://doi.org/10.2198/sbk.24.115

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最近の血漿蛋白質研究の進歩

シユビィックハンスゲルハト

1980 年 24 巻 2 号 p. 121-148
発行日: 1980/09/25
公開日: 2009/03/31

DOIhttps://doi.org/10.2198/sbk.24.121

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SDSゲル電気泳動および抗体を用いた物細胞骨格の研究

オスボーンマリー

1980 年 24 巻 2 号 p. 149-170
発行日: 1980/09/25
公開日: 2009/03/31

DOIhttps://doi.org/10.2198/sbk.24.149

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ニワトリの多量体免疫グロブリン

2.J-chainの精製とその免疫学的,物理化学的性質について

小林邦彦, 平井秀松

1980 年 24 巻 2 号 p. 171-176
発行日: 1980/09/25
公開日: 2009/03/31

DOIhttps://doi.org/10.2198/sbk.24.171

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A homologue of J-chain was purified from chicken serum IgM by ion-exchange chromatography on DEAE-cellulose followed by gel-filtration. Purified chicken J-chain showed a similar molecular weight to human J-chain and its molecular weight was calculated to be 19, 000 daltons.
The amino acid composition of chicken J-chain was also determined and a striking similarity in the content of systeine was found between J-chain of chicken and mammals. However, marked differences in amino acid composition of chicken J-chain were also observed in the contents of glutamic acid, asparatic acid, isoleucine, glycine, leucine and phenylalanine, when compared with those of mammalian J-chains. In spite of such differences in amino acid composition between J-chain of chickens and mammals, a precipitative cross-reaction between J-chain of chickens and human was observed with the antiserum to human J-chain, when reaction was proceeded in agarose gel incorporated with polyethylene glycol (Precipiplex).
NH₂-terminal amino acid could not be detected in the dansylated J-chain, possibly indicating the blocked terminus similar to the human counterpart.
A component which was different from the J-chain in respects of its antigenicity and molecular weight (30, 000 daltons) but which had an electrophoretic mobility similar to J-chain on alkaline-urea-PAGE was identified in the chicken IgM. It was associated with the IgM by disulfide linkage and could possibly be confused with the chicken J-chain.

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リポ蛋白代謝と病態

特にVLDLとHDLとの相互関係について

上田正人, 山崎晴一朗, 戸次久敏, 秋吉俊則, 戸嶋裕徳

1980 年 24 巻 2 号 p. 177-182
発行日: 1980/09/25
公開日: 2009/03/31

DOIhttps://doi.org/10.2198/sbk.24.177

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Eighty-seven patients were divided into two groups such as coronary heart disease (CHD) and non-CHD by the method of coronary angiography. High density lipoprotein-cholesterol (HDL-TCh), very low density lipoprotein total cholesterol/trigryceride (VLDL-TCh/TG) and β-K band were measured in all of the patients after the experimental diet treatment. HDL-TCh in the CHD was significantly lower than that in the non-CHD group, P<0.001. There were, however, no correlation between HDL-cholesterol concentration and the location of coronary arterial stenosis. HDL-TCh and VLDL-TCh/TG were related with the grade of coronary arterial irregularity. An increased grade of the irregularity was accompanied with the lower concentration of HDL-TCh, and the higher ratio of VLDL-TCh/TG. A frequency of the β-K band was significantly higher in the CHD (45.9%) as compared with that in the non-CHD (12.1%). Particularly, twenty-two patients with the irregural coronary artery were showed 68.1%, whereas the patients without the irregularity were only 13.3%. A negative relation between HDL-TCh and VLDL-TG was found in the non-CHD, whereas the CHD was r=0.444.
These results might be of interest in the view of HDL-cholesterol as a well documented and dependent “irregularity of coronary artery.” And this is based on a lipoprotein metabolism correlating between HDL and VLDL fraction. In this investigation a clinical significance of HDL-cholesterol for coronary heart disease has been studied. Particularly, a relationship between the conformation of coronary artery and HDL-TCh was discussed.

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ウシ血清アルブミンのアルカリ変性

堀襄二

1980 年 24 巻 2 号 p. 183-186
発行日: 1980/09/25
公開日: 2009/03/31

DOIhttps://doi.org/10.2198/sbk.24.183

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ウシ血清albのアルカリ変性をアクリルアミドゲル電気泳動(pH9.0)によって研究した.得られたゲル泳動図はアルカリ変性したヒト血清albの泳動図とよく似ており,ウシ血清albの場合もヒト血清albの場合と同様,“firstおよびsecond reaction”が存在するという結論を得た.次に,アルカリ変性したウシ血清albの固有粘度をpH9.0,20℃において測定したところ,上の結論を支持するデータが得られた.

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SDS-ポリアクリルアミドゲル電気泳動による低分子量ポリペプチドの分析法

堀米恒好, 吉田薫, 金井祐子, 長谷川和子, 菅野浩

1980 年 24 巻 2 号 p. 187-190
発行日: 1980/09/25
公開日: 2009/03/31

DOIhttps://doi.org/10.2198/sbk.24.187

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An improved method for the analysis of low molecular weight polypeptides (<molecular weight 10, 000) by SDS-polyacrylamide gel electrophoresis was described. Linear gradient polyacrylamide gels, 10-20% [1:20(w/w) bisacrylamide: acrylamide], with a stacking gel gave good resolution. Separation gels also contained 0.45_M Tris-HCl (pH 8.8), 0.1% SDS and 7_M urea, as well as a 0-10% linear gradient of sucrose. Polypeptides having molecular weights from 1, 400 to 100, 000 could be analyzed by this gel system. Moreover, leakage of low molecular weight polypeptides from gels in the staining procedure had been lowered by using the relatively high concentration of bisacrylmaide. This method could be applied for the analysis and detection of low molecular weight polypeptides from complex biological sources.

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等電点電気泳動における両性担体の分布の数学的解析

島尾和男

1980 年 24 巻 2 号 p. 191-194
発行日: 1980/09/25
公開日: 2009/03/31

DOIhttps://doi.org/10.2198/sbk.24.191

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