生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
24 巻, 4 号
選択された号の論文の11件中1~11を表示しています
  • 橋本 寿美子
    1981 年 24 巻 4 号 p. 289-293
    発行日: 1981年
    公開日: 2009/03/31
    ジャーナル フリー
    Eighty cases of cryoglobulinemia were studied on its components and biological activities, and following conclusions were obtained.
    (1) Eighty cases of cryoglobulinemia were classified into following three types. Type I cryoglobulin which was composed of simple monoclonal immunoglobulin (8 cases). Type II cryoglobulin which was mixed cryoglobulin with a monoclonal component and polyclonal immunoglobulins (19 cases). Type III cryoglobulin which was mixed cryoglobulins without detection of monoclonal component (53 cases).
    (2) The cryoprecipitate was found the largest amount in type I, and the least amount in type III.
    (3) In the 63.1% (12/19) of type II cryoglobulin, M-protein was detected only in the purified cryoprecipitate, but that was not demonstrated in the original serum by immunoelectrophoresis.
    (4) It was suggested that type III cryoglobulin may also contain M-component which could not detected because of the small quantities of cryoprecipitates.
    (5) RF activity was detected in mixed type cryoglobulins (type II, III) at high frequency. But there was no clear differences in the occurrence of ANF activity between the cryoglobulin types.
  • 鄭 一世, 牧野 義彰, 金丸 育恵, 紺野 邦夫
    1981 年 24 巻 4 号 p. 295-300
    発行日: 1981年
    公開日: 2009/03/31
    ジャーナル フリー
    Human transferrin was purified from serum using polyacrylamide gel disc electrophoresis. The purified transferrin appeared to be free from other protein, as judged by electrophoresis on polyacrylamide gel and by immunoelectrophoresis. The purified human transferrin was resolved into a faster moving band (TF) and a slower moving band (TS) by 5.25% polyacrylamide gel electrophoresis. Analysis of the purified human transferrin by isoelectric focusing column separated this iron-transport protein into three components with isoelectric points of 5.61, 5.18 and 4.53. Electrophoresis on 5.25% polyacrylamide gel was carried out on each fraction from the column. Fractions pI 5.61 and pI 5.18 both moved with the same mobility as the slower electrophoretic component (TS). Fraction pI 4.53 moved with the same mobility as the faster electrophoretic component (TF). After recovery from the isoelectric column pI 5.18 transferrin was found to contain 0.90, 1.57 atoms of iron/mol of protein. The pI 4.53 transferrin had less than 0.05 atoms of iron/mol of protein. The pI 5.61 transferrin contained 0.51, 0.65 atoms of iron/mol of protein.
  • 金光 房江, 川西 功躬, 水島 淳
    1981 年 24 巻 4 号 p. 301-308
    発行日: 1981年
    公開日: 2009/03/31
    ジャーナル フリー
    電気泳動的に異常易動度を有するcreatine kinase(CK)の5例について検討した.異常CKは反応液中のcreatine phosphateを除去すると検出されないことより非特異的反応の結果ではないと考えられた.Sephadex G-200 superfineを用いた2種のゲル濾過は異常CKが正常CKよりも高分子であることを示した.Enzyme immunoelectrophoresisとelectroimmunofixationは患者IgAがCKと結合していることを示した.IgAのL鎖はL型に偏っていた.CK-IgAの見かけのKm値がMM型と一致したことより,複合体を構成しているisoenzmeはCK-MMであろうと考えられた.酵素と酵素結合性免疫グロブリンの結合に自己免疫現象の関与が示唆されている現在,donorが総て担癌体であったことは興味深い.
  • 自己免疫疾患と考えられる患者血清について
    松尾 好祥, 本宮 善恢, 岡島 英五郎, 辰巳 聖子, 松岡 洋一, 梅垣 健三
    1981 年 24 巻 4 号 p. 309-312
    発行日: 1981年
    公開日: 2009/03/31
    ジャーナル フリー
  • 西田 利穂, 田中 享一
    1981 年 24 巻 4 号 p. 313-318
    発行日: 1981年
    公開日: 2009/03/31
    ジャーナル フリー
    By using the simple thin layer-PAGE, lactate dehydrogenase (LDH) isozymes in bovine serum were determined during the development of fetal and normal pregnant stage, and the isozymes in serum from patient with downer cow syndrom were determined.
    All five forms of LDH isozymes were obserbed in bovine fetal serum. The total LDH activity and the fast-moving isozyme LDH1 increased in bovine fetal serum during development.
    In the pregnant maternal serum, LDH1, LDH2 and LDH3 were detected, while LDH4 and LDH5 were not detected, in which LDH1 had the greatest percentage. There were no changes of LDH isozyme pattern in bovine serum during the pregnancy.
    The slow-moving isozyme LDH5 and total LDH activities most increased in serum from patients with downer cow syndrom.
  • 門福 強樹, 真鍋 敬, 奥山 典生
    1981 年 24 巻 4 号 p. 319-325
    発行日: 1981年
    公開日: 2009/03/31
    ジャーナル フリー
    A detection method of α-amylase activity after two-dimensional electrophoresis was described. An agar-containing filter paper was overlaid on a slice (2mm in thickness) of a slab gel after two-dimensional electrophoresis. Then a 1mm thick blue starch polymer-containing Sepharose layer was overlaid on the ager-filter paper and kept at 37°C. As α-amylase was detected on the filter paper, the filter paper was dried up and densitometry was easy performed. Further, the stained spots on the filter paper did not decolorize after 6 months' preservation.
    Human body fluid samples (5 sera, 2 salivas, 1 pancreatic juice, and 1 urine) were subjected to the activity staining method. Two to six activity spots of α-amylase were detected. The spots were classified into four main species by their isoelectric points; pI 6.9, pI 6.5, pI 5.9, and pI 5.3. The molecular weights of the spots ranged from 140, 000 to 500, 000.
  • Hepatoma cell line によるα1-Microglobulinの産生とその電気泳動法的解析
    榎本 博光, 伊藤 喜久, 高木 皇輝, 原田 弘智, 河合 忠, 笠原 忠, 佐々木 文章
    1981 年 24 巻 4 号 p. 327-333
    発行日: 1981年
    公開日: 2009/03/31
    ジャーナル フリー
    α1-microglobulin(α1-m)was detected by radioimmunoassay in the culture medium of a hepatoma cell line (C-Hc4). The level of this protein in the culture medium measured by radioimmunoassay increased proportionately with cell numbers. With the addition of puromycin and cycloheximide to the culture medium, the production of this protein was completely blocked. It was, therefore, suggested that this protein was actively produced by hepatoma cells.
    This protein showed a complete immunochemical identity with purified α1-m, serum and urinary α1-m from a patient with renal insufficiency by Ouchterlony immunodiffusion and tandem crossed immunoelectrophoresis.
    In crossed immunoelectrophoresis, this protein migrated at the α1-region, being slightly slower than purified and serum α1-m. On SDS disc electrophoresis combined with immune precipitation, this protein was also found to migrate slightly slower than purified α1-m. These results might be caused by a minor difference of its constituent from that of the purifiedα 1-m during the process of synthesizing this protein in hepatoma cells.
  • 松本 宏治郎, 園田 啓, 加野 象次郎
    1981 年 24 巻 4 号 p. 335-340
    発行日: 1981年
    公開日: 2009/03/31
    ジャーナル フリー
    Separation of immunoglobulin-linked LDH in patient serum was performed by high performance liquid chromatography and post-column enzyme reactor.
    In the case of high speed anion exchange chromatography with IEX-525-QAE (4.0 i. d.×50mm), abnormal chromatograms of IgA-linked LDH and IgG-linked LDH were obtained. Minor difference in separation mechanisms were observed between electrophoretic and anion exchange chromatographic methods.
    In the case of high speed gel permeation chromatography with TSK-5, 000 PW (7.6 i. d.×600mm) and double TSK-3, 000 SW (7.6 i. d.×600mm), IgA-linked LDH was completely separated from normal size of LDH and determination of each LDH activity was performed. Moreover, IgG-linked LDH was separated to two high molecular weight of LDH. This finding will suggest that IgG-linked LDH will be different in its binding forms from IgA-linked LDH.
  • 杉田 雄二, 竹内 拓司, 東 悳彦
    1981 年 24 巻 4 号 p. 341-346
    発行日: 1981年
    公開日: 2009/03/31
    ジャーナル フリー
    免疫学的手法を用いて,メラノーマ細胞におけるチロシナーゼ生合成部位を検索するため,チロシナーゼの精製を行った.細胞のcrude homogenateを78,000g1時間遠心し生じたペレットをトリプシン処理して得たcrude extractから塩析,Sephadex G-100,DEAE-Sephadexおよびcelluloseを用いて約800倍まで精製した.この結果,湿重量90gのメラノーマ細胞から約3mgの精製チロシナーゼを得た.精製標品はポリアクリルアミドゲル電気泳動で,ほぼ単一な蛋白質として染色された.
    精製チロシナーゼを抗原としてウサギを免疫し,抗チロシナーゼ抗血清を得た.抗血清はオクタロニーによりチロシナーゼのみと反応した.
    次にメラノーマ細胞から定法により,遊離型,膜付着型リボソームを調製した.リボソームのA260/A235は1.5~1.6であった.その後,抗チロシナーゼ抗血清を用い,直接法,間接法によりチロシナーゼ合成リボソームを特異的に沈降させた.さらにリボソーム,ATP,GTP,エネルギー再生系,ラット肝臓cell sapからなるcell free系でチロシナーゼの生合成を観察した.その結果,チロシナーゼは90%以上膜分画に存在するにもかかわらず,遊離型,膜付着型リボソームで同程度生合成されていることが観察された.
  • pHモノトニーの法則の証明
    島尾 和男
    1981 年 24 巻 4 号 p. 347-352
    発行日: 1981年
    公開日: 2009/03/31
    ジャーナル フリー
  • 宮谷 勝明
    1981 年 24 巻 4 号 p. 353-355
    発行日: 1981年
    公開日: 2009/03/31
    ジャーナル フリー
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