生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
26 巻, 6 号
選択された号の論文の4件中1~4を表示しています
  • 1982 年 26 巻 6 号 p. 411-449
    発行日: 1982/12/25
    公開日: 2009/03/31
    ジャーナル フリー
  • 第1報:IgD精製法の確立と抗血清の作製について
    荒川 正明, 榎本 博光, 伊藤 喜久, 櫻林 郁之介, 河合 忠
    1982 年 26 巻 6 号 p. 451-456
    発行日: 1982/12/25
    公開日: 2009/03/31
    ジャーナル フリー
    A new method was established for a high yield of purified IgD by using ion exchange chromatography under the conditions where the IgD protein was not absorbed.
    Despite the buffer containing 0.01M ε-amino caproic acid was used to protect against proteolysis, spontaneous degradation of IgD occured during purification step at 4°C. The molecular weight of this degradation fragment was about 123, 000 daltons determined by SDS-PAGE. It was, therefore, probable that the fragments were F (ab)2δ or the dimer of Fcδ and Fabδ.
    For the study of the reactivity aginst Fabδ portion, specific rabbit anti-IgD antibody was prepared and compared with those supplied commercially. The results indicated that the latter did not show any reaction against Fabδ, suggesting that it may lack the antibody against Fdδ.
  • 真鍋 恵子, 真鍋 満久, 須藤 加代子, 菅野 剛史
    1982 年 26 巻 6 号 p. 457-462
    発行日: 1982/12/25
    公開日: 2009/03/31
    ジャーナル フリー
    The physico-chemical and kinetical properties of abnormal pseudocholinesterase (p-ChE) in two families (J. T. and K. K.) were studied and discussed.
    The p-ChE levels of the patient J. T. and K. K. were 10% and 3% of the lower limit of normal ranges, respectively. The p-ChE of patient J. T. was classified as homozygote for atypical gene by the inhibition studies. However, the p-ChE of K. K. showed much larger Km-values for several substrates than normal controls and the Hill's number of the patient's p-ChE was 1.10, though the values less than 1.0 were usually obtained from normal control. Therefore, the p-ChE of K. K. have not the allosteric property with negative cooperativity, as was the nature of normal one. Further studies by isoelectric focusing and slab PAG electrophoresis revealed the traces of the activity of p-ChE in the patient J. T., but any activity band was not detected in the patient K. K. These results indicated that the patient J. T. was homozygote of atypical gene and that the patient K. K. was homozygote for silent gene. It was suggested that the analysis of Hill's number, additional to the conventional inhibition studies, might be useful for the detection of the silent gene of p-ChE in details.
  • 佐子山 豈彦
    1982 年 26 巻 6 号 p. 463-468
    発行日: 1982/12/25
    公開日: 2009/03/31
    ジャーナル フリー
    われわれはキイロショウジョウバエの正常(2系統)と母性効果突然変異(38系統)の卵巣蛋白質をO'Farrellの2次元電気泳動法を用いて分析した.その結果,対立遺伝子の変異を反映するものと考えられるいくつかの蛋白質スポットの変化が認められた.しかしながら母性効果突然変異に直接関係した蛋白質の変化は認められなかった.また,各蛋白質スポットの濃度の変化もいくつか認められたが定量化が困難なため今回は省略した.
    このように母性効果突然変異を直接反映するような蛋白質の変化を検出するには定量化を含めて解像力の改善が望まれる.しかしながら,この方法は簡単で,1部の突然変異の検出には有益な手段である.
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