生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
27 巻, 4 号
選択された号の論文の7件中1~7を表示しています
  • SDS-電気泳動法を用いた可溶性蛋白質の分析とその男性ホルモン依存性変化の差違
    松尾 雄志, 西 望, 田中 幸夫, 六車 謙喜, 和田 文雄
    1983 年 27 巻 4 号 p. 173-179
    発行日: 1983/09/30
    公開日: 2009/03/31
    ジャーナル フリー
    ラットの腹部前立腺(VP)と背側部前立腺(DLP)から可溶性画分を調製し,含まれる蛋白質をSDS-電気泳動法によって分離した.分離された種々の蛋白質が去勢および去勢後のテストステロン投与(以後,+Tと略す)によって変化する様子を解析し,VPとDLPの間における差を調べた.実験にはオスのSD系ラット(14~15週齢)を用いた.蛋白質種の含量は可溶性画分中の蛋白質100μgを分析し,得られたデンシトグラム上での相対面積値から求めた.
    1.含量が最大の蛋白質の変化:VPにおける最大含量の蛋白質,16K(分子量約1.6万のポリペプチド),の含量は去勢によって減少したが,DLPにおける最大含量の蛋白質,67K,は増加した.2.特異的な蛋白質の変化:VPに特異的な13K,14Kおよび20Kの含量は去勢によって減少し,+Tによって正常に戻った.しかし,DLPに特異的な蛋白質の中で,30K含量は去勢によって増加し,+Tによって正常以下に減少した.また,120K含量は去勢および+Tによってほとんど影響を受けなかった.以上の様に,VPとDLPの間には著しい差が認められ,これらの蛋白質の去勢による変化はVPにおいて早く現われ,一方,+Tによる変化はDLPにおいて早く現われ,組織重量の変化とよく一致した.
  • ラット各臓器組織の比較
    貞広 荘太郎, 高見 博, 高橋 哲也, 小平 進, 石村 巽, 阿部 令彦
    1983 年 27 巻 4 号 p. 181-188
    発行日: 1983/09/30
    公開日: 2009/03/31
    ジャーナル フリー
    The cellular proteins from various organs of rats were studied by two-dimensional (isoelectric focusing-SDS PAGE) electrophoresis. More than one hundred of peptide spots were detected in each organ. Although many of the peptide spots were common among the organs studied, some of them were quantitatively and/or qualitatively different from one organ to another. These peptide spots indicate the potential existence of organ-associated cellular proteins.
  • 戸沢 辰雄, 佐藤 仁美
    1983 年 27 巻 4 号 p. 189-193
    発行日: 1983/09/30
    公開日: 2009/03/31
    ジャーナル フリー
    We developed a simple, reliable and sensitive method for the identification of the class and type of amylase-linked immunoglobulin in patients with macromylasemia. This method is based on the principles of immunoprecipitin reaction in free liquid media, thereby the class and type of amylase-linked immunoglobulin were easily identified from amylase activity in the immunoprecipitate.
    Each specific antiserum and serum specimen were mixed at an optimum ratio. Free amylase admixed in the immunoprecipitate thus formed was completely removed by repeated washings with veronal buffer. To the washed immunoprecipitate a color reagent (suspension of Bluestarch polymer) was added directly. After incubation, absorbance of the supernatant was measured at 620nm to obtain the activity of amylase in the immunoprecipitate.
    Of the 12 cases with macroamylasemia studied, only two cases permitted identification of both the H- and L-chain of amylase-linked immunoglobulin by an enzyme-immunoelectrophoresis technic, whereas, according to this method, both chains were identified in all of the cases.
  • 戸沢 辰雄, 柴田 宏
    1983 年 27 巻 4 号 p. 195-199
    発行日: 1983/09/30
    公開日: 2009/03/31
    ジャーナル フリー
    A simple and sensitive method is presented for detection of alkaline phosphatase (ALP)-linked immunoglobulin in human serum. This method is based on the empirical observation that ALP activity of ALP-linked IgG has been present on the anode side of IgG immunoprecipitin arc by immunoelectrophoresis.
    The procedure is as follows; 3μl each of the serum sample and antiserum are applied on the commercial agarose gel film, and then electrosyneresis is carried out. After washing out free ALP in the gel film with saline solution containing 1.75mM MgCl2, the film is incubated with ALP staining reagents. ALP-linked immunoglobulin is detected by ALP activity on the immunoprecipitin line.
    By this method, ALP-linked immunoglobulin was detectable even after ALP VI had disappeared by isoenzyme analysis. It was superior to conventional methods (enzyme-immunoelectrophoresis and enzyme-immunoprecipitin reaction in free liquid media) in sensitivity and simplicity of procedure. The present mothod was proved to be the most useful for the detection of ALP-linked immunoglobulin, especially for the screening test.
  • 増田 治美, 塚田 敏彦, 中山 年正, 北村 元仕
    1983 年 27 巻 4 号 p. 201-207
    発行日: 1983/09/30
    公開日: 2009/03/31
    ジャーナル フリー
    A search for macroamylasemia has been routinely carried out on the serum samples ordered to perform amylase isozyme analysis (“the ordered samples”) and the out-patients' specimens with hyperamylasemia (≥200S.U./dl). Of 41 macroamylasemia which were picked up over a period of 15 months, 38 cases were determined to be due to amylase-binding immunoglobulins and types of the immunoglobulins were identified. Incidence of amylase anomaly in the hyperamylasemic sera from out-patients was much higher than that in “the ordered samples”. No significant relations were found between the macro to total ratio of amylase activity and the level of total amylase activity. As for the heavy chain of the amylase-binding immunoglobulin, α-chain (IgA) occupied 74% (28 cases), and κ-type was dominant in its light chain. Amylase bound to IgG and IgA were frequently positioned in their electrophoretic mobilities at post-P and fast-S, respectively. As for the feature of the patients with macroamylasemia, males from 50 through 70 years of age were predominant, and malignancy, gout and diabetes mellitus were frequently found.
  • 免疫複合体という観点から
    橋本 寿美子, 橋本 正勝, 河野 均也
    1983 年 27 巻 4 号 p. 209-214
    発行日: 1983/09/30
    公開日: 2009/03/31
    ジャーナル フリー
    In this study, a mixed type cryoglobulin (IgM-κ/polyclonal IgG) was analyzed to demonstrate which one of the components had the antibody-like activity to the other, and we tried to analyze the mechanisms of the uniqe thermal characteristics of the cryoglobulin. The following results were obtained.
    1) The IgM-protein and the polyclonal IgG obtained from the cryoglobulin did not form cryoprecipitate at 4°C separately.
    2) From the recombination experiment of the two cryoglobulin components, it was demonstrated that IgM M-protein and the IgG reacted with each other in nearly 1:1 molar ratio.
    3) The cold precipitation reaction was also demonstrated when the IgM-protein from the cryoglobulin was added to the polyclonal IgG and its Fc fragment from normal subject and kept at 4°C.
    4) Fabμ, (Fcμ)5 and IgMs prepared from the IgM M-protein were not able to form cryoprecipitate with polyclonal IgG.
    5) All of the reactions of the IgM M-protein with hyarulonidase treated IgG, neuraminidase treated IgG and heated IgG resulted in formation cryoprecipitates which did not redissolve on warming at 37°C.
    Based on the above findings, the IgM M-protein was considered to react with polyclonal IgG as a cryoprecipitating factor in this case.
    The properties of the antigenic determinant on the IgG molecule may play an immportant role in the occurence of the thermal reaction. The carbohydrate moiety of IgG Fc is one of the very interesting themes for understanding the thermal properties of the cryoglobulin.
  • 戸田 年総, 藤田 敬子, 大橋 望彦
    1983 年 27 巻 4 号 p. 215-217
    発行日: 1983/09/30
    公開日: 2009/03/31
    ジャーナル フリー
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