All issues
Successor
Volume 27 (1983)
- Issue 6 Pages 289-
- Issue 5 Pages 229-
- Issue 4 Pages 173-
- Issue 3 Pages 109-
- Issue 2 Pages 51-
- Issue 1 Pages 1-
Volume 27, Issue 6
Displaying 1-6 of 6 articles from this issue
- |<
- <
- 1
- >
- >|
-
Takashi Kanno1983 Volume 27 Issue 6 Pages 289-293
Published: December 30, 1983
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (3828K) -
1983 Volume 27 Issue 6 Pages 295-330
Published: December 30, 1983
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (26482K) -
Asao Koide1983 Volume 27 Issue 6 Pages 331-336
Published: December 30, 1983
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA horizontal sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was applied to the study of apolipoprotein components of the human serum very low density lipoprotein (VLDL). By this method, tetramethylurea(TMU)-soluble apolipoproteins could be separated into four major fractions, called apolipoprotein CI (apo CI), CII, CIII and E from anode to cathode.
The distribution of the major soluble apolipoproteins of VLDL in 9 healthy adults as percentage (mean±SD) of the total soluble apolipoprotein was: apo CI, (7.5±3.8): apo CII, (19.8±6.3): apo CIII, (50.0±6.7) and apo E, (22.7±5.4).
The composition of apolipoproteins of VLDL in 9 patients with hypertriglyceridemia was characterized by a decrease of apo CII (13.5±3.1) and an increase of apo CI (10.4±2.3). The results suggest that the variation of apo CII and CI content carried on VLDL may be critical for their interaction with lipoprotein lipase.View full abstractDownload PDF (3962K) -
Motoo Matsuda1983 Volume 27 Issue 6 Pages 337-342
Published: December 30, 1983
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSPurification and physical reconstitution of U1 RNA-containing U1-snRNP of human KB cells were accomplished.
For the purpose of obtaining highly purified U1-snRNP particles, two newly improved methods were used. One is fractionation of nuclear extract by ultracentrifugation on a 5-20% linear sucrose density gradient in anticipation of concentrating snRNPs in the low molecular weight fraction (about ≤12S). The other is anti-RNP antibody-immunosorbent affinity column chromatography accompanied by stepwise elution of increasing salt concentrations.
The “single U1 RNA-containing U1-snRNP particle” was recovered from the column by elution with 2.5M MgCl2 at pH 7.2. Its S value was estimated as about 9-10S by analysis of physical endogenous reconstitution.View full abstractDownload PDF (3561K) -
Masaharu Isobe, Zen-ichi Ogita1983 Volume 27 Issue 6 Pages 343-347
Published: December 30, 1983
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSIsoelectric points of mouse zymogens were determined by two-dimensional electrophoresis using isoelectric forcusing and in situ activation of zymogens. To prevent a gel form swelling and breaking during staining of protein or two-dimensional electrophoresis, an isoelectric focusing gel formed on a hydrophilic polyester sheet was used. A new two-dimensional electrophoretic condition was developed to analyze isoelectric points of inactive zymogens. First, zymogens in the pancreatic extract were separated by isoelectric focusing. And then, after activation of zymogens on a one-dimensional gel, second electrophoresis was carrid out on another polyacrylamide gel layer. Following detection of protease activities using both methods of non-specific and specific staining, isoelectric points of zymogens were determined from positions of the first run. Determined isoelectric points of inactive zymogens, such as trypsinogen-II (Try G-II), chymotrypsinogen-I (Chy G-I) and chymotrypsinogen-II (Chy G-II) were 3.9, 4.6 and 4.4, respectively. And moreover, it was shown that trypsinogen-I group (Try G-I group) was derived from two trypsinogens, Try G-Ia and Try G-Ib which have different isoelectric points of 4.9 and 4.7, respectively.View full abstractDownload PDF (3765K) -
Jun Suzuki, Sadao Kobayashi1983 Volume 27 Issue 6 Pages 349-354
Published: December 30, 1983
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSCellulose acetate membrane isoelectric focusing (CAIEF) of streptolysin O from Group A streptococci and direct detection method of hemolytic activity by red blood cell agar layering after focusing are described. Eleven hemolytic components from concentrated culture filtrate were detected which had pI values of 4.8, 5.0, 5.3, 5.4, 5.6, 5.9, 6.4, 6.6, 6.9, 7.2 and 7.4. Sensitivity of the direct detection method of hemolytic activity was examined with pI 6.5 hemolytic component prepared by preparative gel isoelectric focusing. This method was extremely sensitive, which was possible to detect 0.4 HD50 of streptolysin O. These methods were applied to the analysis of the fractions obtained by preparative polyacrylamide or Sephadex gel isoelectric focusing of concentrated culture filtrate. Reproducibility of pI value in CAIEF was comparable to that in polyacrylamide gel isoelectric focusing. The method is inexpensive and enables easy detection of hemolytic toxin.View full abstractDownload PDF (3061K)
- |<
- <
- 1
- >
- >|