巻号一覧
後続誌
27 巻 (1983)
- 6 号 p. 289-
- 5 号 p. 229-
- 4 号 p. 173-
- 3 号 p. 109-
- 2 号 p. 51-
- 1 号 p. 1-
27 巻, 6 号
選択された号の論文の6件中1～6を表示しています
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菅野 剛史1983 年 27 巻 6 号 p. 289-293
発行日: 1983/12/30
公開日: 2009/03/31
ジャーナル フリーPDF形式でダウンロード (3828K) -
1983 年 27 巻 6 号 p. 295-330
発行日: 1983/12/30
公開日: 2009/03/31
ジャーナル フリーPDF形式でダウンロード (26482K) -
小出 朝男1983 年 27 巻 6 号 p. 331-336
発行日: 1983/12/30
公開日: 2009/03/31
ジャーナル フリーA horizontal sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was applied to the study of apolipoprotein components of the human serum very low density lipoprotein (VLDL). By this method, tetramethylurea(TMU)-soluble apolipoproteins could be separated into four major fractions, called apolipoprotein CI (apo CI), CII, CIII and E from anode to cathode.
The distribution of the major soluble apolipoproteins of VLDL in 9 healthy adults as percentage (mean±SD) of the total soluble apolipoprotein was: apo CI, (7.5±3.8): apo CII, (19.8±6.3): apo CIII, (50.0±6.7) and apo E, (22.7±5.4).
The composition of apolipoproteins of VLDL in 9 patients with hypertriglyceridemia was characterized by a decrease of apo CII (13.5±3.1) and an increase of apo CI (10.4±2.3). The results suggest that the variation of apo CII and CI content carried on VLDL may be critical for their interaction with lipoprotein lipase.抄録全体を表示PDF形式でダウンロード (3962K) -
松田 基夫1983 年 27 巻 6 号 p. 337-342
発行日: 1983/12/30
公開日: 2009/03/31
ジャーナル フリーPurification and physical reconstitution of U1 RNA-containing U1-snRNP of human KB cells were accomplished.
For the purpose of obtaining highly purified U1-snRNP particles, two newly improved methods were used. One is fractionation of nuclear extract by ultracentrifugation on a 5-20% linear sucrose density gradient in anticipation of concentrating snRNPs in the low molecular weight fraction (about ≤12S). The other is anti-RNP antibody-immunosorbent affinity column chromatography accompanied by stepwise elution of increasing salt concentrations.
The “single U1 RNA-containing U1-snRNP particle” was recovered from the column by elution with 2.5M MgCl2 at pH 7.2. Its S value was estimated as about 9-10S by analysis of physical endogenous reconstitution.抄録全体を表示PDF形式でダウンロード (3561K) -
磯部 正治, 荻田 善一1983 年 27 巻 6 号 p. 343-347
発行日: 1983/12/30
公開日: 2009/03/31
ジャーナル フリーIsoelectric points of mouse zymogens were determined by two-dimensional electrophoresis using isoelectric forcusing and in situ activation of zymogens. To prevent a gel form swelling and breaking during staining of protein or two-dimensional electrophoresis, an isoelectric focusing gel formed on a hydrophilic polyester sheet was used. A new two-dimensional electrophoretic condition was developed to analyze isoelectric points of inactive zymogens. First, zymogens in the pancreatic extract were separated by isoelectric focusing. And then, after activation of zymogens on a one-dimensional gel, second electrophoresis was carrid out on another polyacrylamide gel layer. Following detection of protease activities using both methods of non-specific and specific staining, isoelectric points of zymogens were determined from positions of the first run. Determined isoelectric points of inactive zymogens, such as trypsinogen-II (Try G-II), chymotrypsinogen-I (Chy G-I) and chymotrypsinogen-II (Chy G-II) were 3.9, 4.6 and 4.4, respectively. And moreover, it was shown that trypsinogen-I group (Try G-I group) was derived from two trypsinogens, Try G-Ia and Try G-Ib which have different isoelectric points of 4.9 and 4.7, respectively.抄録全体を表示PDF形式でダウンロード (3765K) -
鈴木 潤, 小林 貞男1983 年 27 巻 6 号 p. 349-354
発行日: 1983/12/30
公開日: 2009/03/31
ジャーナル フリーCellulose acetate membrane isoelectric focusing (CAIEF) of streptolysin O from Group A streptococci and direct detection method of hemolytic activity by red blood cell agar layering after focusing are described. Eleven hemolytic components from concentrated culture filtrate were detected which had pI values of 4.8, 5.0, 5.3, 5.4, 5.6, 5.9, 6.4, 6.6, 6.9, 7.2 and 7.4. Sensitivity of the direct detection method of hemolytic activity was examined with pI 6.5 hemolytic component prepared by preparative gel isoelectric focusing. This method was extremely sensitive, which was possible to detect 0.4 HD50 of streptolysin O. These methods were applied to the analysis of the fractions obtained by preparative polyacrylamide or Sephadex gel isoelectric focusing of concentrated culture filtrate. Reproducibility of pI value in CAIEF was comparable to that in polyacrylamide gel isoelectric focusing. The method is inexpensive and enables easy detection of hemolytic toxin.抄録全体を表示PDF形式でダウンロード (3061K)
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