The cellular proteins from various human organs were analyzed by two-dimensional (isoelectric focusing-SDS PAGE) electrophoresis. More than one hundred of polypeptide spots were detected in each organ. Although a unique polypeptide map was obtained from each organ, the patterns of stomach and colon, both originating from the same germ layer and possessing columnar epithelium, were relatively similar. The patterns of heart muscle and skeletal muscle, both originating from the same germ layer and possessing the ability of contraction, were quite different from each other. Those of liver and kidney were similar, although the organs were originated from different germ layer and possessed different functions and cell types. Therefore, the patterns in various organs could not be classified according to their histology, functions and embryology.
There were three spots (molecular weight×10
-3/isoelectric point: 55/6.0, 43/7.6, 38/8.0), which were commonly found in all of the organs except for the blood. These spots might be species-associated cellular proteins.
There were several qualitatively or quantitatively characteristic spots in each organ. These might be organ-associated cellular proteins.
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