生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
29 巻, 1 号
選択された号の論文の13件中1~13を表示しています
  • 原 三郎
    1985 年 29 巻 1 号 p. 1-6
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
  • 横山 三男
    1985 年 29 巻 1 号 p. 7-13
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
  • 山崎 晴一朗
    1985 年 29 巻 1 号 p. 15-16
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
  • 櫻林 郁之介
    1985 年 29 巻 1 号 p. 17-21
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
  • Linda K. Curtiss
    1985 年 29 巻 1 号 p. 23-33
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
    Mouse monoclonal antibodies that bind either human apolipoprotein (apo) AI or human apo AII were characterized with respect to their binding of high density lipoprotein (HDL) particles in solution. The single apo AII-specific antibody bound 85% of 125I-HDL and 100% of soluble 125I-apo AII. However, none of the three apo AI-specific antibodies bound greater than 60% of either HDL or soluble apo AI. Limiting amounts of antibody or antigen, radioiodination of the ligands, and unavailability of the epitopes for reaction with antibody were not responsible for the incomplete binding of HDL and apo AI, suggesting the existance of immunochemical heterogeneity of apo AI as organized on HDL as well as free apo AI in solution. The validity of this heterogeneity was supported by demonstrating that: i) increased binding of HDL occurred when each of the apo AI antibodies were combined to from an oligoclonal antibody mixture, and ii) 100% binding of HDL occurred when two apo AI antibodies were combined with the single apo AII antibody. These studies demonstrated that all apo AII peptides in plasma HDL possess at least one epitope in common, whereas the apo A I peptides in HDL are immunochemically heterogenous.
  • 等電点電気泳動法を中心に
    山村 卓
    1985 年 29 巻 1 号 p. 35-41
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
  • PBC (原発性胆汁性肝硬変) 患者の異常リポ蛋白について
    古賀 俊逸, 宮田 康司
    1985 年 29 巻 1 号 p. 43-49
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
  • 上田 正人, 谷口 孝治
    1985 年 29 巻 1 号 p. 51-56
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
    The purpose of this study is to demonstrate clinical significances, lipid composition of plasma lipoproteins, and plasma apoprotein concentrations in the patients with abnormal apoproteinemia (two apo E-3 deficiency, one patients with apo E-5, and two hypoalphalipoproteinemias). Apo E isoform was analyzed by the analytical isoelectric focusing of apo-VLDL using 7.5% of polyacrylamide gel in each subject. Plasma apo A-I, A-II, B, C-II, and E concentrations were measured by the single radial immunodifusion methods. Lipid concentrations of each plasma lipoproteins were measured after the isolation by the ultracentrifuge. Two patients with apo E-3 deficiency were type IV and II hyperlipoproteinemia, and the latter was diabetic treated with sulfonylurea. The diabetic female had a cholesterol-rich VLDL, and her plasma apo E was 12.4mg/dl, whereas another one male showed normal ratio of VLDL-TC/TG and apo E concentration. One chronic thyroidism had apo E-5, and his plasma apo E was 5.2mg/dl. However, his plasma apo B concentration was significantly higher than that of the healthy volunteers. He had no evidence of cardiovascular disease. His daughter has hyperthyroidism, but her apo E isoform was normal and plasma apo E level was also normal. One familial hypercholesterolemia and one hypertriglyceridemia showed hypo α-lipoproteinemia. Their plasma apo A-I, II were significantly lower, and apo B were markedly higher than those of the control, respectively. It is possible that the lower concentration of HDL-cholesterol can be based on the decreased HDL3-cholesterol rather than HDL2-cholesterol. The paper discuss their back grounds of abnormal apoproteinemia.
  • 大滝 義博, 渡辺 博夫, 吉田 充輝, 志村 憲助
    1985 年 29 巻 1 号 p. 57-65
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
    遺伝情報発現に重要な役割をはたすと考えられている非ヒストン蛋白質 (NHP) の構造と機能を明らかにする目的の第1歩として豚胸腺からNHPを大量分離する為の条件と回収された核蛋白質のSDS電気泳動による分析を行った. 豚胸腺からクロマチンを単離後, 5M Urea, 2M NaCl, 0.05M Tris-HCl, 2mM 2-mercaptoethanol 中で93%の核蛋白質をDNAから解離させた. これに塩化ランタンを加えてDNAを選択的に沈殿させる条件を検討した結果, 0.7%塩化ランタン, pH7.9の条件で98%のDNAが沈殿として, また, 91%の核蛋白質が上清に回収された. 上清に残った核蛋白質はpHを8.6に上げるのみで75%が沈殿として回収できた. pHを8.6に上昇させた後の上清 (pH8.6 Sup.) と沈殿 (pH8.6Ppt) に含まれる蛋白質分子種の異同を知る目的で, 両者をSP-Sephadex C-25によるNaCl濃度の段階的上昇により溶出させたのち各溶出画分をSDS電気泳動で分析した所, 大部分のNHPはSP-Sephadex C-25の0.25Mおよび0.3MNaCl溶出画分に溶出すること, pH8.6 SupとpH8.6Ppt画分で異なるのはヒストン画分のみであることが明らかとなった. これにより, NHPの分離の目的にはpH8.6Ppt. 画分を使用しうること, さらに, これまで, その性質が明らかとなっていないDNAに強固に結合したNHPを分離する方法としても本法が使用できることがわかった.
  • 大井 絹枝
    1985 年 29 巻 1 号 p. 67-73
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
    We studied clinical significance of alkaline phosphatase isozyme, ALP1, which appeared in sera of patients with malignant diseases. Incidence of ALP1 was high in lung, esophageal, bile duct, gallbladder cancer, malignant lymphoma and leukemia. In contrast it was low in thyroid, mammary, prostate cancer and myeloma. ALP1 often appeared in post operative stages and the activity of ALP1 increased within a month after operation of solid cancers and of abdominal benign diseases. In the cases of solid cancers ALP1 peak appeared within 6-16 days after operation. However, in the cases of abdominal benign diseases, its appeared 3 to 10 days after operation.
    We observed relationship between incidence of ALP1 and liver function or LDH isozyme in haematopoietic malignant diseases. In both normal and abnormal liver groups, the incidence of ALP1 was higher in the cases with tumor pattern LDH isozyme (increase in LDH2 and/or LDH3) than those with the normal pattern.
  • 松田 基夫, 藤谷 英男
    1985 年 29 巻 1 号 p. 75-81
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
    The U1-snRNP particles from the nuclear extracts of human KB cells and mouse teratocarcinoma embryoid bodies (OTT6050), purified by SLE-anti-(U1)RNP antibody affinity chromatography, were analyzed for their protein components. The purified human U1-snRNP was recovered from the column by elution with 2.5M MgCl2 at pH7.2 after the column had been washed with Tris-buffer containing DOC, Triton X-100 and 1.0M NaCl. The 3H amino acid-labeled protein components of human (KB) U1-snRNP resolved into three major bands with apparent molecular weights of 69, 000, 58, 000 and 35, 000 upon SDS-polyacrylamide gel electrophoresis.
    Highly purified U1-snRNP particles containing U1a and U1b RNAs as their sole nucleic acid moieties were obtained from the mouse teratocarcinoma embryoid bodies by the same procedure as used for purification of KB cell U1-snRNP. By gel electrophoresis, five major polypeptides, with apparent molecular weights of 80, 000, 76, 000, 72, 000 58, 000 and 39, 000, were detected associated with U1-snRNP(s) of embryoid bodies.
  • 新津 ひさえ, 中屋 敷徳, 熊谷 礼子, 桂 秀策
    1985 年 29 巻 1 号 p. 83-88
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
    サウナで採取した健康な男性54名の汗についてPAG平板ディスク電気泳動し, CBB染色とPAS染色を行い, 次の結果を得た. ヒト汗には血清に相当するバンドが全例において認められ, α-glob 領域には汗の重要な成分と思われる2個のバンドがみられた. α-, β-, γ-各 glob 領域で汗バンドの多様性がみられた. pre-alb 領域には移動度の異なる3種の糖蛋白と思われるものがあった.
  • 住 幸治, 直居 豊, 田中 卓雄, 片山 仁
    1985 年 29 巻 1 号 p. 89-98
    発行日: 1985/03/15
    公開日: 2009/03/31
    ジャーナル フリー
    Time sequential changes of various serum protein fractions of rabbits irradiated in a single dose of 800R of 60Co γ-ray, were evaluated by electrophoresis, immunoelectrophoresis and single radial immunodiffusion. The results are as follows;
    (1) The increase of serum albumin level was observed immediately after irradiation. However, serum albumin level decreased significantly afterwards. On the statistical analysis, significant correlation between serum albumin level and other protein fractions was recognized.
    (2) The significant decrease of α1-globulin level was encountered immediately following irradiation.
    (3) Time sequential changes of serum α2-globulin were opposite to those of serum albumin level.
    (4) The significant increase of β-globulin was recognized for certain period following irradiation.
    (5) The day of irradiation significant decrease of serum γ-globulin level was obtained in immediate and first day after irradiation.
    This phenomenon was thought to be related to IgG value.
feedback
Top