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Volume 29 (1985)
- Issue 6 Pages 349-
- Issue 5 Pages 287-
- Issue 4 Pages 225-
- Issue 3 Pages 179-
- Issue 2 Pages 105-
- Issue 1 Pages 1-
Volume 29, Issue 4
Displaying 1-7 of 7 articles from this issue
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Kawai Tadashi1985 Volume 29 Issue 4 Pages 225-230
Published: August 15, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (2030K) -
Mituru Takanami1985 Volume 29 Issue 4 Pages 231-236
Published: August 15, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (2896K) -
Kazumi Okuyama, Nobuyuki Honma, Kazuko Hasegawa, Hiroshi Sugano, Iku S ...1985 Volume 29 Issue 4 Pages 237-243
Published: August 15, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSSeveral basic conditions for electrophoretic analysis of the low molecular weight polypeptides in urine and plasma on SDS-polyacrylamide gels were studied. For concentration of uric polypeptides below 30, 000KD, acetone precipitation method was superior to trichloroacetic acid or sulfosalicylic acid method. Blue cellulose could not be used to remove selectively albumin from plasma, because several polypeptides below 30, 000KD were also highly adsorbed by Blue cellulose. Electrophoretic patterns of urines from nephropathy patients were unchanged by standing at room temperature for periods exceeding 20h without the addition of protease inhibitors. Dependence on the resolving power in the low molecular weight region on the pH of separation gel buffer was examined over the pH range 8.52 to 9.12. It decreased when the pH exceeded 8.9, while high resolution was kept up to 8.52 in the lower pH side. Accordingly, it was advisable to use the pH8.65 to 8.70 to obtain constantly the high resolution in the low molecular weight region.View full abstractDownload PDF (6161K) -
Hiroshi Yoshida, Masataka Ono1985 Volume 29 Issue 4 Pages 245-252
Published: August 15, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSExtensive studies on the population of PGM1 polymorphism in human red cells have been studied. Three commonly phenotypes designated as PGM11-1, PGM12-1 and PGM12-2, have been characterized by using the starch gel electrophoresis. However, some rare phenotypes i. e., PGM13-1, PGM1 5-1, PGM16-1, and PGM18-1, have not fully characterized.
We conducted the study of PGM16-1 and PGM16-2 types in red cell lysates from members of a Japanese family.
The proband of the variant was a patient with liver diseases during a medical treatments. From this family of ten individuals, three showed PGM16-1 type and one had a PGM16-2 type. Our experimental method for the determination of PGM1 isoenzymes consisted of starch gel electrophoresis and isoelectric focusing using polyacrylamide gel with a pH range 5∼7. This paper report an unusual phenomenon of three bands which are characterized for PGM16 phenotype by isoelectric focusing when two bands are generally obtained by starch gel electrophoresis for the same individuals.View full abstractDownload PDF (4198K) -
Takanori Moriyama, Manabu Nobuoka, Takaaki Takebe, Yoshio Shimojima1985 Volume 29 Issue 4 Pages 253-260
Published: August 15, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe immunochemical properties of the macroamylases found in seven patients were examined. The class of heavy chain of the amylase-linked immunoglobulin was proved to be alpha in six cases and gamma in one case. The type of light chain was determined to be lambda in four cases, kappa in two cases, and kappa-lambda in one case. All the Ig A-amylase complexes were completely dissociated by Con-A affinity chromatography. The zymograms of dissociated amylase were normal. When free immunoglobulins released by acidification were mixed with purified human pancreatic or salivary amylase, the immunoglobulins recombined with both amylases in five cases, but only with pancreatic amylase and only with salivary amylase in one case, respectively. The precipitin line of Ig A-amylase complex, which was digested with papain, against anti-human Ig A/F (ab')2 serum was proved to have amylase activity, but that against anti-human Ig A/Fc serum was proved not to have amylase activity. The result highly suggests that in Ig A-amylase complex amylase molecule links with Fab portion of Ig A molecule.View full abstractDownload PDF (2893K) -
Atsuo Mori, Zen-ichi Ogita1985 Volume 29 Issue 4 Pages 261-265
Published: August 15, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA preparative procedure for obtaining highly purified plasmid DNA from bacterial cells is described. Rapid preparation of milligram quantities of plasmid DNA is achieved by ethidium-bromide (EtBr)-cleared lysate formation, polyethyleneglycol (PEG) precipitation, and chromatography through a BioGel A-50m column. The average yield of pBR322 DNA from 1 liter of amplified culture by this procedure is 1.8mg and the preparation is highly pure. This method is applied to the cloning of the human β-globin gene cluster.View full abstractDownload PDF (2011K) -
Kazuo Muroi, Ryuhei Sasaki, Yasusada Miura, Haruhiro Yoshida, Shigenor ...1985 Volume 29 Issue 4 Pages 267-270
Published: August 15, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSElectrophoretic pattern of human leucocyte cell extracts was surveyed by isotachophoresis.View full abstractDownload PDF (413K)
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