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Volume 29 (1985)
- Issue 6 Pages 349-
- Issue 5 Pages 287-
- Issue 4 Pages 225-
- Issue 3 Pages 179-
- Issue 2 Pages 105-
- Issue 1 Pages 1-
Volume 29, Issue 6
Displaying 1-13 of 13 articles from this issue
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Immunological identification of electrophoretic componentsKazumi Okuyama, Hiroshi Sugano, Fumitake Gejyo, Masaaki Arakawa1985 Volume 29 Issue 6 Pages 349-354
Published: December 25, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSUrines from Fanconi's patient (as a example of tubular proteinuria) were electrophoresed on the SDS-polyacrylamide gel showing high resolving power in the low molecular weight range, and the identification of indivisual protein bands after an electrophoretic separation was performed by an immunological method. Urinary proteins were radiolabeled by reductive methylation with 14C-formaldehyde and sodium cyanoborohydride. Immunoprecipitates were obtained from 14C labeled urinary proteins to which antisera against serum proteins characteristic of nephropathy and then formalin-fixed S. aureus cells were added. Immunoprecipitates were then electrophoresed and analyzed by fluorography. A fluorogram of 14C labeled urinary proteins resembled a silver-stained electrophoretic pattern of urinary proteins. Among the major bands having molecular weights of 67K, 52K, 42K, 28K, 22K, 16.7K, and 8.9K, the following bands were identified; albumin (67K), α1-acid glycoprotein (42K), α1-microglobulin (28K), retinol-binding protein (22K), and β2-microglobulin (8.9K). But, β2-microglobulin showed a molecular weight of 11.2K on electrophoresis after the urinary proteins were treated with reducing agent.View full abstractDownload PDF (4948K) -
Takumitsu Hiro, Tsuyoki Kadofuku, Yoshiaki Makino, Tsuneo Sato, Kunio ...1985 Volume 29 Issue 6 Pages 355-362
Published: December 25, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe changes of various components and properties in blood by a 10 km-run physical exercise were examined using six volunteers. The levels of RBC, WBC, Ht, Hb, MCV, reticulocyte, osmotic pressure, lactic acid, and CPK significantly increased after the exercise. A plasma protein which remarkably appeared after the exercise was detected by two-dimensional gel electrophoresis. This plasma protein was detected at the position of pI 5.0, molecular weight of about 70, 000 on two-dimensional gel under non-denaturing condition, but it showed a molecular weight of about 30, 000 on SDS-polyacrylamide gel. No remarkable changes were observed in two-dimensional electrophoretic patterns of cytosol and cell membrane protein before and after the exercise.View full abstractDownload PDF (7707K) -
Osamu Ohue, Shigeki Nakamura, Kazue Abe1985 Volume 29 Issue 6 Pages 363-368
Published: December 25, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA new method has been devised for the electrophoretic transfer of proteins from the PAGIEF gel to a pair of nitrocellulose membranes which placed on the both sides of the gel. With repeatedly changing over the polarities of electrodes of the apparatus in accordance with the timetable, this method enabled to equally transfer the proteins to a pair of nitrocellulose membranes simultaneously. Furthermore, it was possible to equally quantitatively transfer the proteins of molecular weight about 50, 000∼120, 000 to two pairs of nitrocellulose membranes.
The results indicate that the both-sides blotting is applicable in the studies of genetic polymorphism of plasma proteins and identification of protein antigens following two-dimensional gel electrophoresis.View full abstractDownload PDF (3321K) -
A highly sensitive method by using peroxidase labeled antibodyToshiho Nishita, Nozomu Hibi1985 Volume 29 Issue 6 Pages 369-374
Published: December 25, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA sensitive sandwich enzyme immunoassay method for measurement of rat α-fetoprotein(AFP) was established by use of affinity purified antibodies to rat AFP. The assay system consisted of filter paper discs with immobilized antibodies and the same antibodies labeled with horseradish peroxidase(POD). The optimal conditions for the assay and for the preparation of POD labeled antibodies were determined. The assay was as sensitive as radioimmunoassay(RIA) and rat AFP at concentrations of 5 to 1280ng/ml was measurable. AFP values obtained by the present method were in good agreement with those by RIA and the correlation coefficient was 0.99.View full abstractDownload PDF (597K) -
Chihiro Sekiya, Atsushi Takahashi, Masayoshi Namiki, Kazuhisa Taketda, ...1985 Volume 29 Issue 6 Pages 375-380
Published: December 25, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSTwo members of a Japanese family (male and female) with serum pseudocholinesterase (ChE) activities elevated two to three times of the normal mean were studied for isozyme pattern by polyacrylamide gel slab and gradient gel slab electrophoresis. Beside the normal ChE bands, additional bands were observed: one between minor C3 and C4 bands, one cathodally to major C5 and one (or two) cathodally to C6 band. All the bands disappeared upon treatments with antibodies to normal ChE and also with ethopropazine. The serum ChE had normal dibucaine and fluoride numbers. In view of the fact that the properties of ChE isozyme in the present cases do not correspond to any of the described one, the variant ChE was named E. Takamatsu after its place of origin.View full abstractDownload PDF (4648K) -
Kazuo Umetsu, Tsuneo Suzuki1985 Volume 29 Issue 6 Pages 381-384
Published: December 25, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe method for protein fixation using lectin in place of antibody, designated as lectinofixation, was developed. By this method, allo A lectin-binding human serum proteins following two-dimensional electrophoresis and isoelectric focusing were detected. By the lectinofixation with allo A, the main components of allo A-binding proteins such as orosomucoid, α1-antitrypsin, transferrin and haptoglobin were detected, while albumin was not detected. This technique is easier to perform in a shorter period of time and has been found to be a useful tool in detection of allo A lectin-binding glycoproteins.View full abstractDownload PDF (3537K) -
Tatsuo Tozawa, Hitomi Satoh1985 Volume 29 Issue 6 Pages 385-389
Published: December 25, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSIt is very rare that amylase-linked immunoglobulin(Ig) is detected in serum of infant or child. Amylase-linked IgG-κλ in sera of the two infants (two months old and eleven months old infants) were detected. Their amylase isoenzyme patterns show taling. Amylase-linked Ig was detected by the immunoprecipitin reaction. But it was not detected in serum of their mothers. In two months old infant, amylase-linked Ig was not detected in the cord serum at birth.
On the other hand, amylase-linked IgG was detected in serum of newborn delivered from the mother with amylase-linked IgG-κ. But, amylase-linked IgA was not detected in serum of newborn delivered from the mother with amylase-linked IgA-κ, but in the milk of the mother.
These results suggest that serum amylase-linked Ig in these two infants may be produced in themselves, not delivered from the mothers.View full abstractDownload PDF (500K) -
Tatsuo Tozawa, Tamiko Futami, Hiroshi Shibata1985 Volume 29 Issue 6 Pages 391-395
Published: December 25, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA relationship between enzyme-linked immunoglobulins and autoantibodies were studied.
Twenty-two patients with Ulcerative colitis (UC) who were positive for enzyme-linked immunoglobulins (E-Igs), 75 cases with non-autoimmune diseases who were positive for E-Igs, 10 healthy individuals positive for E-Igs and 28 E-Igs negative patients with UC were subjected to the screening of the eight types of autoantibodies, i.e., Rheumatoid antibody(RA), Lupus erythematodes antibody(LE), Anti-nuclear antibody(ANA), Antideoxyribonucleotide antibody(DNA), Antithyroglobulin antibody(ATG), Anti-microsome antibody(AMC) Anti-mitochondrial antibody(AMA), and Anti-smooth muscle antibody(ASMA).
At least one type of these autoantibodies was found in 68% of the E-Igs positive UC patients, 60% of the cases with non-autoimmune diseases, 30% of healthy individuals and in 46% of the UC patients negative for E-Igs.
The detection rates of autoantibodies in terms of respective E-Igs were 67% in the CK-Ig cases, 48% in the amylase-Ig patients, 59% in the AP-Ig cases and 66% in the LD-Ig patients.
When compared with the UC cases negative for E-Igs, significantly higher rates of detection were observed in such autoantibodies as RA in the CK-Ig positive UC cases, AMA in the AP-Ig positive UC patients and ANA in the UC cases positive for LD-Ig (p<0.05).
The results of the present studies suggested a relationship between E-Igs and autoantibodies in patients with ulcerative colitis.View full abstractDownload PDF (542K) -
Itsuo Kuroda, Chinami Nakazawa, Kohsuke Mori, Mitsutaka Yoshida1985 Volume 29 Issue 6 Pages 397-402
Published: December 25, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA case with testicular carcinoma (S.G., 22 years old man, Clinical diagnosis: Embryonal carcinoma), total serum LDH activity being 7 times higher than that of normal upper range, was reported. Using a fractional analysis of the serum LDH, it was demonstrated that LDH-I fraction which assumed to be derived from the tumor tissue was significantly elevated (maximum about 90%). In the next step, biochemical properties of this serum LDH was investigated. As to attitudes against various inhibitors, Michaelis constant(Km), optimum pH and thermostabilities in 37, 65°C, this LDH was identical with normal LDH or LDH in a patient with myocardial infarction. This LDH, however, activated rather than maintained its enzymatic activity for about 80 minutes in 56°C, demonstrating a stronger than thermal stability of standard LDH of myocardial type.View full abstractDownload PDF (2158K) -
Itsuo Kuroda, Chinami Nakazawa, Kohsuke Mori, Mitsutaka Yoshida1985 Volume 29 Issue 6 Pages 403-406
Published: December 25, 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSColor developmental condition in LDH isoenzyme assay using agarose gel film as medium was investigated fundamentally. Optimum conditions for this assay were pH8.6, DL-litium lactate concentration 120∼150mM/l, NAD concentration 4∼5mM/l and tricine concentration 0.1M/l. These conditions were found to be identical for those of Pol-E film method. However, optimum concentrations of diaphorase enzyme used as an intermediate electron carrier was 7, 500U/l and that of NTB was 0.5mM/l. Furthermore, the color developmental reaction in agarose gel film was a linear at least in 60 minutes. After these improvements, an assay condition with better color developmental sensibility and quantitability was obtained in comparison with Pol-E film method.View full abstractDownload PDF (355K) -
1985 Volume 29 Issue 6 Pages 413a
Published: 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (861K) -
1985 Volume 29 Issue 6 Pages 413b
Published: 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (861K) -
1985 Volume 29 Issue 6 Pages 413c
Published: 1985
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (861K)
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