生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
30 巻, 4 号
選択された号の論文の10件中1~10を表示しています
  • 梶井 英治, 山口 祐司, 植木 寿一, 吉田 治弘, 三浦 恭定, 池本 卯典
    1986 年 30 巻 4 号 p. 247-250
    発行日: 1986/08/15
    公開日: 2009/03/31
    ジャーナル フリー
    On the red blood cell membrane in a patient with autoimmune hemolytic anemia (AIHA) several glycophorin class blood group antigens were assumed to be acquired in relation to the disease. When the red blood cells were treated with Dispase, all of the acquired antigens were released to the supernatant in company with the native glycophorin class antigens and disappeared on the red blood cell membrane. Then a trial was made to analyze the supernatant of the red blood cell suspension with Dispase and the serum which contained the red cell autoantibody by capillary isotachophoresis. In each of the isotachopherogram, a specific peak which was not detected in the sample from normal human was found in the acidic zone. The protein substances expressed by these peaks will be available as a marker to diagnose AIHA because they are thought to be formed in association with AIHA.
  • 桜井 秀子, 須藤 優子, 稲毛 博実, 渡辺 孝太郎, 小山 哲夫, 成田 光陽, 東條 静夫
    1986 年 30 巻 4 号 p. 251-255
    発行日: 1986/08/15
    公開日: 2009/03/31
    ジャーナル フリー
    In order to identify urinary proteins excreted from normal subjects, we employed a new method by which molecular weight and immunochemical property of urinary proteins can be determined simultaneously. Urine was subjected to linear gradient (3∼40%) SDS-PAGE and then transferred to nitrocellulose membrane by electrophoretic blotting method. The membrane was stained with Auro Dye and blotted proteins were identified by enzyme immunoassay using specific antibodies. In this method, we identified more than 12 protein bands from urine, that is, α2-macroglobulin, tubular epithelial antigen, Tamm-Horsfall glycoprotein, IgA, IgG, transferrin, albumin, α1-microglobulin, L-chain (dimer, monomer), retinol-binding protein, and β2-microglobulin. In addition, we found native forms and some fragments of immunoglobulins. In this report, we analyzed only normal subjects.
    These results suggest that this method is useful for analysis not only of glomerular or tubular proteins but also tissue antigens from urine with various kinds of renal diseases.
  • 成田 光陽, 小山 哲夫, 桜井 秀子, 須藤 優子, 稲毛 博実, 紅露 恒男, 東條 静夫
    1986 年 30 巻 4 号 p. 257-263
    発行日: 1986/08/15
    公開日: 2009/03/31
    ジャーナル フリー
    The urinary proteins from healthy adults were analyzed in different age groups, using gradient gel SDS-PAGE and electrophoretic blotting methods. The proportions of low molecular proteins increased as the age became elder, and this phenomenon began at fifties. Electrophoretic blotting analysis showed that the increased proteins were identified as retinol-binding protein, free-light chain (monomer and dimer), which were defined as tubular proteinuria.
    From the above observations, we speculated that the increase in excretion of low molecular weight proteins by ageing, are mainly due to tubulopathy. This appear predominantly at fifties and gradually progress during ageing.
  • 山田 隆紹, 高田 浩暢, 松田 基夫, 佐俣 哲郎, 松原 利光
    1986 年 30 巻 4 号 p. 265-272
    発行日: 1986/08/15
    公開日: 2009/03/31
    ジャーナル フリー
    The hemopexin from feline plasma was purified using a simple method and partially characterized. The purified hemopexin was recovered (about 13-23%) from Wheat germ Lectin Sepharose column by elution with 10% N-acetylglucosamine and judged to comprise 100% of the protein in the purified fraction by the analysis of immunoelectrophoresis and immunodiffusion. The feline hemopexin, with approximate molecular weight of 70, 000 was observed by Laemmli gel electrophoresis.
    In order to partially characterize the purified hemopexin, first we carried out the analysis of amino acid composition. A profile of the composition showing essentially some similar features to that of human and rabbit before reported, was observed.
    Secondaly, the levels of serum hemopexin during growth of cat were determined. A tremendous increase of the concentration was observed for 4 months after birth and the hemopexin have already attained to an adult level at 4 months. On the other hand, the total serum protein of cat increased slightly for the corresponding periods.
    Moreover, the gene regulation of hemopexin during development of cat and the physiological function (s) were also described.
  • 菰田 二一, 小山 岩雄, 岡野 こずえ, 三浦 雅一, 坂岸 良克
    1986 年 30 巻 4 号 p. 273-281
    発行日: 1986/08/15
    公開日: 2009/03/31
    ジャーナル フリー
    We are re-evaluated here the characteristics of human urinary alkaline phosphatases (ALP).
    According to the results of wheat germ agglutinin-affinity electrophoresis, serial lectin affinity chromatographies, isoelectric point, molecular weight determination, and immunological identification, the urinary ALPs from healthy adults and patients with hepatoma were similar to the natures of liver and/or bone like ALP. In the case of the patients with chronic nephritis or acute nephritis, the ALPs contained a major band of kidney like ALP with a minor band of bone and intestinal ALPs. But, the ALPs in pregnant woman had not only liver or bone ALP but also placental like ALP.
    On the other hand, most of workers for urinary ALPs have been claimed that the molecular weight of urinary ALP is smaller than those of organic original ALPs. However, present data suggested that the molecular weight of urinary ALPs is well accorded to those of organic ALPs, except for the enzyme from the patient with acute nephritis. Moreover, total activity of the urinary ALP was close-related to that of the serum ALP.
    Consequently, in general, urinary ALP may be derived from serum ALP by minor modification, suggesting that the excreated ALP in urine depend upon the half-life for respective ALP isozymes in blood stream.
  • 本間 信幸, 菅野 浩, 下条 文武, 荒川 正昭, 伊東 義一
    1986 年 30 巻 4 号 p. 283-288
    発行日: 1986/08/15
    公開日: 2009/03/31
    ジャーナル フリー
    Serum ultrafiltrate was obtained from hemodialysis patients by using an extra corporeal ultrafiltration method equipped with a hollow fiber dialyser, and electrophoresed on the SDS-polyacrylamide gel having a high resolving power in the low molecular weight region. Among the major bands less than 40KD observed with plasma of hemodialysis patient, the bands of 29K, 28K, 25K, 22K, 16.7K and 8.9K molecular weights were also found in serum ultrafiltrate of the same patient. By an immunological method, the following bands were identified: α1-microglobulin (28K), retinol-binding protein (22K), prealbumin (16.7K) and β2-microglobulin (8.9K). As compared with serum ultrafiltrate obtained from healthy person, 28K, 25K (in some cases), 22K and 8.9K bands showed obvious increase in cases of hemodialysis patients except for one patient who showed an urine volume of 200ml/day and an electrophoretic pattern closed to that of healthy person.
  • その性質とトライアスロン競技における変動
    弘 卓三, 門福 強樹, 牧野 義彰, 佐藤 永雄, 岩根 久夫, 紺野 邦夫
    1986 年 30 巻 4 号 p. 289-294
    発行日: 1986/08/15
    公開日: 2009/03/31
    ジャーナル フリー
    A specific protein which appeared in plasma after a 10km-run was detected using two-dimensional electrophoresis, and it's properties were investigated. This specific protein was detected at almost the same position as albumin on two-dimensional gel under non-denaturing condition, but it was not adsorbed to anti-albumin-Sepharose column. The molecular weight was estimated to be 25, 000 on SDS-polyacrylamide gel electrophresis. This protein was stained with periodic acid-Shiff reagent but not with Sudan Black. This protein was also detected in triathlon race, but it's increase did not definite.
  • 土田 修一, 西海 栄一, 吉田 治弘, 池本 卯典
    1986 年 30 巻 4 号 p. 295-300
    発行日: 1986/08/15
    公開日: 2009/03/31
    ジャーナル フリー
    ミクロ2次元電気泳動法によるヒトの耳下腺唾液Pa、Pr蛋白について遺伝的多型の検出法を検討した。すなわち、ミクロ2次元電気泳動法によりヒト耳下腺唾液を試料として泳動すると、等電点4~5付近にPaおよびPr蛋白の多型を検出することが可能である。その表現型はpH range 3.5~5.2の尿素スラブポリアクリルアミドゲルを支持体とした等電点電気泳動法によって検出されるPaおよびPrの表現型と同様であった。
  • 佐々木 博, 吉田 光孝, 降矢 〓
    1986 年 30 巻 4 号 p. 301-308
    発行日: 1986/08/15
    公開日: 2009/03/31
    ジャーナル フリー
    患者血清中に乳酸脱水素酵素 (LDH; EC1.1.1.27) の活性阻害因子が存在すること, またその阻害因子は免疫グロブリンであり, 主に A-subunit に対する阻害がみられた例が報告されている. 我々は, 血清LDH活性はほとんど検出できなかったが赤血球LDH活性が正常に認められた心筋梗塞の既往歴のある症例に遭遇した.
    この症例の血清中のLDH阻害因子について生化学的・免疫化学的性質に関する解析を行った. その結果, この阻害因子はトリス緩衝液(pH7, 4) に対して透析されず, 56℃, 60分間の熱処理・クエン酸緩衝液によるpH解離実験・3M尿素および各SH試薬処理などによる変化はみられなかった. この阻害因子を保存する血清を, ヒト赤血球から精製したLDHアイソザイムに添加すると各アイソザイムに95~99%の酵素活性阻害が認められた. さらにLDH結合免疫グロブリンの検索により従来の報告にみられない異常免疫グロブリンG, λ(IgGλ) 結合型であり, LDH-Aおよび-B subunit の両者に対する活性阻害が観察された. 5'-AMP Sepharose 4Bおよび Protein A-Sepharose Cl-4Bなどを用いて分離・精製された患者血清中のIgGは, 他の血清LDHに対して阻害効果を示し, さらにこの異常IgGのサブクラスのIgG3も同様の阻害効果を示した.
  • 1986 年 30 巻 4 号 p. 316
    発行日: 1986年
    公開日: 2009/03/31
    ジャーナル フリー
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