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Volume 32 (1988)
- Issue 6 Pages 285-
- Issue 5 Pages 215-
- Issue 4 Pages 163-
- Issue 3 Pages 113-
- Issue 2 Pages 71-
- Issue 1 Pages 1-
Volume 32, Issue 3
Displaying 1-8 of 8 articles from this issue
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Masamichi Oh-ishi, Tamio Hirabayashi1988 Volume 32 Issue 3 Pages 113-120
Published: June 30, 1988
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA procedure was established for micro-two-dimensional gel electrophoresis with agarose gels in the first dimension. Agarose (Agarose IEF, Pharmacia) was previously found to be suitable for the analysis of high molecular weight proteins with a wide range of isoelectric points, but rather difficult to be handled for micro-scale electrophoresis. However, addition of 1M thiourea in combination with 5M urea to the medium for agarose gel made it possible to be used with ease on micro-scale as well as on macro-scale. Since thiourea is a potent protein solubilizing reagent, almost all amounts of proteins including structural proteins were presented on the respective positions on two-dimensional gels. This method is applicable to other experiments with high molecular weight proteins to be analyzed in a wide range of isoelectric points.View full abstractDownload PDF (5372K) -
Michinari Yokohama, Yasuko Watanabe1988 Volume 32 Issue 3 Pages 121-124
Published: June 30, 1988
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe protease inhibitor (Pi) types in light-breed horses were studied by isoelectric focusing (IEF) and two-dimensional (2-D) electrophoresis. Since equine Pi types are highly heterogeneous, establishment of confirmatory techniques for correctly distinguishing each Pi type has been an important issue, and 2-D electrophoresis was found to be effective for this. Each Pi type with PiS and PiT alleles was subjected to 2-D electrophoresis, and their characteristics were clearly shown in photographic form.View full abstractDownload PDF (3591K) -
Yoko Arai, Iwao Koyama, Yoshikatsu Sakagishi, Tsugikazu Komoda1988 Volume 32 Issue 3 Pages 125-130
Published: June 30, 1988
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSCharacteristics of rabbit kidney cortex and medulla alkaline phosphatases (ALPs) were compared. Although two ALPs had similar enzymatic properties, the mobilities on polyacrylamide gel electrophoresis and sugar moieties of the cortex ALP differed from those of the medulla enzyme. These findings were well consistent with those of human cortex and medulla ALPs.
On the other hand, the antigenicity for ALPs revealed that the cortex ALP reacted to both anti-human liver and anti-human intestinal ALP antibodies, whereas the medulla ALP to not only the anti-human intestinal but also anti-human placental ALP antibodies. Furthermore, rabbit kidney ALPs did not fuse with either anti-rat liver or intestinal ALP antibodies. It is possible to suggest that gene expression of rabbit kidney ALPs are different from the other mammalian kidney ALPs.View full abstractDownload PDF (2279K) -
Shuichi Tsuchida, Kazuyoshi Uematsu, Emiko Nozawa, Atsushi Nagai, Shig ...1988 Volume 32 Issue 3 Pages 131-134
Published: June 30, 1988
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe polymorphism of group-specific component (Gc) in Japanese monkeys (Macaca fuscata fuscata) was analyzed by two-dimensional gel electrophoresis. Two phenotypes were detected by two-dimensional gel electrophoresis. These were designated as Gc 2 and Gc 2-2A1 according to the nomenclature described by Constans et al.1) in comparison with the phenotypes detected by convensional isoelectric focusing electrophoresis followed by immunofixation with anti-human Gc. The phenotype frequencies in ten wild Japanese monkeys were 70% for type Gc2 and 30% for type Gc2-2A1. The Gc typing in Japanese monkeys by two-dimensional gel electrophoresis is a useful and inexpensive method than convensional isoelectric focusing electrophoresis followed by immunofixation with antiserum.View full abstractDownload PDF (3631K) -
Analysis of the proteins by two-dimensional gel electrophoresis and Western blotting methodHiroaki Asaga, Satoko Kozawa, Atsushi Ebara, Tsutomu Inoue1988 Volume 32 Issue 3 Pages 135-140
Published: June 30, 1988
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSTo confirm the migration of proteins from the albumen gland to the egg of the land snail, Euhadra, the soluble proteins extracted from albumen glands and eggs of E. peliomphala, E. subnimbosa and E. quaesita were analyzed immunochemically.
The major polypeptide groups (named No. 8 and 9 polypeptide groups on the polypeptide map) of soluble proteins extracted from the albumen gland of E. peliomphala were purified and used to immunize rabbits. The specific antibodies against the albumen gland proteins (anti-AG) were purified from the antiserum. The soluble proteins extracted from the albumen glands and the eggs of E. peliomphala, E. subnimbosa and E. quaesita were analyzed by O'Farrell's two-dimensional gel electrophoresis and Western blotting method with anti-AG. The anti-AG reacted not only with the two polypeptide groups, which were the same as the immunogens used, but also with another polypeptide group (named No. 14 polypeptide group on the polypeptide map) of all the samples tested. These results support strongly the migration of these polypeptide groups from the albumen gland to egg in Euhadra.View full abstractDownload PDF (3562K) -
Futoshi Shibata, Tsutomu Uchiyama, Zen-ichi Ogita1988 Volume 32 Issue 3 Pages 141-144
Published: June 30, 1988
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe effect of gelatin that was added into agarose gel on the separation of DNA is reported. Addition of gelatin R (pI 8.4) to agarose gel caused interference with migration of DNA fragments. Addition of modified gelatin S9, which was prepared by succinylation of the amino groups of gelatin R, resulted in appearance of molecular sieving effect and separation of DNA fragments at rates that were inversely proportional to log10 of molecular weights. The interference due to addition of gelatin R may be caused by interaction between DNA and the amino groups of gelatin. The same effect was observed for the gel made solely of gelatin R. The DNA fragments separated in gelatin R gel were easily recovered by phenol treatment and subsequent precipitation with ethanol.View full abstractDownload PDF (3373K) -
Shin-ichi Inoue1988 Volume 32 Issue 3 Pages 145-152
Published: June 30, 1988
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe method of fine separation of alpha chains of collagen types I, III, and V by means of delayed reduction of collagen type III with thioglycolic acid (TG) during electrophoresis was investigated. This method is simple and rapid because it does not necessitate an interruption of electrophoresis for the reduction of type III collagen. Furthermore, precise quantitation of alpha chains of collagen type I [alpha1 (I)] and type III [alpha1 (III)] can be achieved even with small sample quantities which contain other types of collagen because the mobility of alpha1 (III) is determined by varying the time TG addition to running buffer for the delayed reduction.View full abstractDownload PDF (3791K) -
Shuichi Tsuchida, Kazuyoshi Uematsu, Emiko Nozawa, Atsushi Nagai, Shig ...1988 Volume 32 Issue 3 Pages 153-155
Published: June 30, 1988
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (2111K)
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