All issues
Successor
Volume 34 (1990)
- Issue 6 Pages 289-
- Issue 5 Pages 241-
- Issue 4 Pages 185-
- Issue 3 Pages 133-
- Issue 2 Pages 53-
- Issue 1 Pages 1-
Volume 34, Issue 6
Displaying 1-13 of 13 articles from this issue
- |<
- <
- 1
- >
- >|
-
Hiroshi Mizusawa1990 Volume 34 Issue 6 Pages 289-292
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (5485K) -
Wolfgang Schütt, Uwe Thomaneck, Eberhard Knippel, Joachim Rychly, ...1990 Volume 34 Issue 6 Pages 293-300
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe use of automated devices in the last ten years have made biomedical applications of cell electrophoresis more attractive. We are using routinely the automated single cell electrophoresis microscope PARMOQUANT. Using the PARMOQUANT we applied this method to discriminate lymphocytes and study the interaction of substances with cells and synthetic particles. Electrophoretic histograms allowed the determination of changes in the proportion of lymphocyte populations after kidney transplantation, during dialysis treatment, open heart surgery and during pregnancy. Discrimination of leukemic cells on the basis of electrophoresis was used as an additional parameter in diagnosis. In a mouse tumor model histograms determination enabled evaluation of the in vivo effect of tumor necrosis factor on immune cells. Cell electrophoresis was shown to be suitable to detect the influence of antibodies, lectins and bacteria on the cell surface. Protein adsorption was studied on synthetic particle using cell electrophoresis. This method was applied in investigating the phenomena of blood interaction with biomaterials for use in artificial organs and to determine differences in the protein composition in serum or other body fluids connected with diseases. On the basis of this principle a test to detect hetrozygotes in cystic fibrosis is now in progress.View full abstractDownload PDF (10983K) -
Jun-ichi Asakawa1990 Volume 34 Issue 6 Pages 301-305
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (6476K) -
Shinobu Imajoh-Ohmi1990 Volume 34 Issue 6 Pages 307-315
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (5723K) -
Takashi Manabe1990 Volume 34 Issue 6 Pages 317-320
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (3903K) -
Tamio Hirabayashi, Jun-Ichi Miyazaki, Takatoshi Inoue, Masashi Kirinok ...1990 Volume 34 Issue 6 Pages 321-327
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSTwo-dimensional gel electrophoresis with agarose in the first dimension was criticized from a view point of applicability to various materials. The method was found useful in separating most protein components of muscle, liver, kidney and lens in wide ranges of pI and molecular weight, leaving no protein trapped on the top of the first dimension gel. Although there was some difficulty with plant materials and cell nuclear preparations, beautiful separation patterns were obtained with most materials and analyzed by an image analysis system to demonstrate many isoforms of skeletal muscle troponin T and tropomyosin, coordination or otherwise in accumulation between troponin subunits, T and C, and between lens crystallin isoforms, and tissue-specific distribution of tropomyosin isoforms.View full abstractDownload PDF (7058K) -
Kazuyuki Nakamura, Ryousuke Suzuno, Tatehiko Tanaka, Masanori Fujimoto ...1990 Volume 34 Issue 6 Pages 329-333
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (5515K) -
Tosifusa Toda, Mochihiko Ohashi, Toshiko Fujita, Mitsutaka Yoshida, Ya ...1990 Volume 34 Issue 6 Pages 335-340
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (5198K) -
Shinzo Kobayashi, Nobuo Hiratuka, Kimiko Tanaka, Teruko Otake, Shojiro ...1990 Volume 34 Issue 6 Pages 341-347
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSWe have tried to establish an expert system which is based mainly on computerized analysis of serum protein electropherogram to comprehend conditions of patients. The method consists of the quantitative analysis of digital signals from the automated electrophoresis system and of the total visualization of parameters. Seventeen parameters were selected, i. e., fractions (5), A/G ratio, total protein (TP), top & bottom positions of each fraction (9) and half width of albumin fraction. A standard normal pattern, which becomes a base of a superimposed pattern, was derived from 562 electropherograms of normal samples in absolute value equalized by the total protein. And the standard basic parameters in SD index were obtained by calculation of (measured value-mean value)/standard deviation. The SD index was comparable to each parameter in the common unit. The range over ±3SD was defined as abnormal level for each parameter. Application of this system to typical abnormal serum protein patterns showed that it was very effective to demonstrate their abnormalities both quantitatively and graphically.View full abstractDownload PDF (811K) -
Kimiko Tanaka, Teruko Otake, Shojiro Kano, Hisami Iri, Shinzo Kobayash ...1990 Volume 34 Issue 6 Pages 349-355
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSBiochemical parameters, ZTT, TTT, TC, TB, CA, ALB, CRP, IgG, IgA, IgM, LDH and GOT, were added to serum protein electrophoresis data to make up a more general expert system. Experimental analysis of typical abnormal serum protein patterns using this new system, revealed several advantages; One was easiness in understanding electrophretic abnormalities through graphic presentation in the absolute value superimposed over the standard pattern. Another was the availability of the SD index which was shown in both numeric and graphic forms. Particularly graphic presentation of the SD index was effective to demonstrate the condition of a patient in both biochemical and electrophoretical aspects. Using a microcomputer the modal mobility which signified top & bottom positions of each fraction could be reported in SD index quantitatively, and successfully accompanied with several parameters which were shown to be helpful for further characterization of it.View full abstractDownload PDF (804K) -
Atsushi Hiraoka, Isao Miura1990 Volume 34 Issue 6 Pages 357-361
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSAn analytical system for separating sennosides A and B was established using capillary-isotachophoresis. These are the major purgative components of Rhei Rhyzoma (daiou; the rhyzomes of Rheim palmatum and related species) and Sennae Folium (Senna; the leaves of Cassia angustiflorum and related species). Under the conditions employed, the zone of sennoside A was clearly separated from that of its stereoisomer, sennoside B, and the sennosides in the crude drug extracts were identified by mixed charging with authentic samples. The sennoside A and B contents in a total of 30 Rhei Rhyzoma and Sennae Folium specimens were then determined by this system, and a part of them (10 specimens, in total) was also treated by the thin-layer chromatographic method. The results showed that the present capillary-isotachophoresis system is powerful, at least to the same degree as the conventional thinlayer chromatographic system, as a tool for rapid and microscale analysis for use in pharmacognosy.It can be used to measure the major sennoside contents in a large number of crude drug specimens.View full abstractDownload PDF (589K) -
Tsuyoshi Saito1990 Volume 34 Issue 6 Pages 363-371
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSAlpha-amylase of amniotic fluid was studied with the pH titration curve method using the conventional enzyme active staining. Four clear amylase bands, which have different isoelectric points ranging from 4.9 to 6.5, were recognized on 5% polyacrylamide gel. Three bands of them had the same isoelectric point of deamidated salivary alpha-amylase bands, but did not clearly change their isoelectric points after neuraminidase treatment. Under continuous pH range, the affinity electrophoresis was also performed by combination with this method. Main alpha-amylase band of amniotic fluid, similar to that of saliva, showed higher affinity to the ligand than that of pancreas.View full abstractDownload PDF (6487K) -
Toyoji Sato, Masatake Suzuki, Sadahiko Akai, Hiromichi Kuniki, Tetsuo ...1990 Volume 34 Issue 6 Pages 373-376
Published: December 15, 1990
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (1845K)
- |<
- <
- 1
- >
- >|