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Volume 35 (1991)
- Issue 6 Pages 417-
- Issue 5 Pages 339-
- Issue 4 Pages 253-
- Issue 3 Pages 155-
- Issue 2 Pages 59-
- Issue 1 Pages 1-
Volume 35, Issue 4
Displaying 1-12 of 12 articles from this issue
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Tatsuo Tozawa, Junko Kuwahara1991 Volume 35 Issue 4 Pages 253-257
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSAnomalous LDH electrophoretic pattern in serum from a patient with malaria tertian, an additional band being present between LDH 3 and LDH 4, apparently due to the existence of malarial LDH is described. To our knowledge, this phenomenon has not been reported. The band was found to be eluted with normal LDH isoenzymes in the serum during gel filtration, and its activity was lactate dependent. However, data on enzymatical and immunochemical properties of the band indicated that it differed from human LDH. The band was also found in a hemolysate from the patient's blood cells, and the band appearance in the patient's serum and hemolysate was associated with the presence of maralia parasites in the erythrocytes.View full abstractDownload PDF (4234K) -
Comparison of the liver and heart muscleTaizo Hayashi, Yasutaka Kaneda, Keiko Hirokawa, Toru Watanabe, Takao T ...1991 Volume 35 Issue 4 Pages 259-264
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSWe measured aminotransferase activity and vitamin B6 content in the liver and heart muscle of diabetic mice. Two different types of diabetic mice, spontaneously non-obese diabetic (NOD) and alloxan-induced diabetic mice, were used. Control mice were either non-diabetic NOD or ICR. We reported a marked increase in aspartate aminotransferase (AST) activity in the liver of diabetic mice compared with that of control mice. The diabetic livers also had more vitamin B6 than normal livers: pyridoxamine (PM) levels were particularly high but pyridoxal levels were not. There were no significant differences in AST activity and vitamin B6 of the heart muscle in the diabetic mice compared with control mice. The abundance of AST and B6 in the diabetic liver is consistent with the great need for gluconeogenic substrate there. A correlation between s-AST and PM was significantly recognized in the diabetic liver, while not in normal mice.View full abstractDownload PDF (634K) -
Kazuhisa Taketa, Hiroko Taga, Hidematsu Hirai1991 Volume 35 Issue 4 Pages 265-269
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe presence of Aleuria aurantia lectin (AAL)-reactive alpha-fetoprotein (AFP) was demonstrated in human sera by affinity electrophoresis. Although AAL has been shown to react strongly with glycoproteins having fucosylated core N-acetylglucosamine (GlcNAc), AFP reacting with lentil lectin-A, which has a similar sugar chain specificity, did not necessarily show affinity for AAL. Fucosylated outer chain GlcNAc was suggested as an alternative oligosaccharide structure of AFP having affinity for AAL.View full abstractDownload PDF (2843K) -
Yosuke Kishimoto, Sadako Yamada, Takeaki Suou, Chisato Hirayama1991 Volume 35 Issue 4 Pages 271-274
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe phenotype of haptoglobin and α1-antitrypsin was analyzed by polyacrylamide gel electrophoresis and isoelectric focusing on polyacrylamide gel in 100 healthy donors, 78 patients with chronic hepatitis and 83 patients with liver cirrhosis. The cirrhosis patients, especially the decompensated patients had an excess haptoglobin 1 gene and α1-antitrypsin M3 gene. Statistical analysis revealed that the combined gene of haptoglobin 1 and α1-antitrypsin M3 regulates the severity of chronic liver diseases resulting from chronic viral infection.View full abstractDownload PDF (1649K) -
Keiko Yamaguchi, Hideo Asakawa1991 Volume 35 Issue 4 Pages 275-277
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSImmunostaining method for the sensitive detection of isoferritins separated by isoelectric focusing on cellulose acetate membrane is described in this paper. Methanol was used in order to fix proteins to the membrane. Membrane was shaked in methanol immediately after isoelectric focusing and then incubated in phosphate buffered saline containing a sufficiency of antibody and 2% skim milk. Blocking, a step for filling in the blank of membrane was not necessary. Fine isoferritin profile with clear background was obtained using this procedure. The sensitivity of immunostaining was slightly higher than that of gold staining in which proteins were fixed with sulfosalicylic acid and/or trichloroacetic acid. When 250ng of liver ferritin was applied on the membrane, minor acidic isoferritin bands also were observed distinctly.View full abstractDownload PDF (3130K) -
Kazuo Shimao1991 Volume 35 Issue 4 Pages 279-283
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA simple method of preparation of wedge-shaped agarose gel was devised. Thickness of the gel was calibrated by densitometry of gel containing China ink. In zone electrophoretic separation of serum proteins using barbital-N-methyl-D-glucamine buffer at pH8.6 and ionic strength 0.045, band sharpening of prealbumin and albumin fractions was observed when thick end of the gel was directed toward anode in accordance with the theoretical prediction.
In isoelectric focusing of serum protein fractions using wide range carrier ampholyte (Sepaline pH3.5-10, Fuji Photofilm), spacings of fractions were increased in thin end and reduced in thick end of the gel compared to those obtained by the flat gel. Thus, the effects obtained by the use of wedge-shaped gel were similar to those of additions of narrow range carrier ampholytes in flat gel isoelectric focusing.View full abstractDownload PDF (2245K) -
A quantitative assay for hTFIID mRNA based on polymerase chain reaction (PCR)Chieki Wada, Hideki Ohtani1991 Volume 35 Issue 4 Pages 285-290
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA general transcription factor IID (TFIID) binds to the TATA box promoter element and regulates the expression of most eukaryotic genes transcribed by RNA polymerase II (Pol II). A highly sensitive, specific and quantitative assay for human TFIID (hTFIID) mRNA was developed based on polymerase chain reaction (PCR). The distinctive points of our procedure include the use of small amount of total cellular RNA (1μg), a random primer for cDNA synthesis, β2-microglobulin (β2M) as an internal control and calculation of the relative value of hTFIID transcript from 32P-incorporation of the co-amplified PCR at different cycles. By this procedure, distribution of the hTFIID gene expression was for the first time demonstrated in normal human tissues and the amount of hTFIID mRNA was measured. In some tissues such as liver, fetal lung and placenta, moderate levels of hTFIID mRNA were detected. hTFIID transcript appeared correlated to total mRNA initiation and protein synthesis in tissue. This quantitative PCR procedure can be applied to more extensive studies of gene expression.View full abstractDownload PDF (3020K) -
Kazusuke Takeo, Masanori Fujimoto, Isao Suzuki, Tatehiko Tanaka, Kazuy ...1991 Volume 35 Issue 4 Pages 291-296
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSBy use of a accurately thermostated disc gel electrophoresis apparatus, apparent dissociation constants of various biospecific interactions at varying temperatures were calculated. From the van't Hoff plots, thermodynamic constants were calculated. The obtained values well coincided with those values which were obtained by other techniques, such as equilibrium dialysis or fluorescence quenching and enhancement technique.
Affinity of hydrophilic interactions such as those between concanavalin A and dextran or glycogen phosphorylase and glycogen, decreases with a rise in temperature of the reaction medium. In these interactions, standard enthalpy change, ΔH°, and standard entropy change, ΔS°, were calculated as negative one. On the contrary, affinity of hydrophobic interaction, such as those between aromatic hapten and anti-aromatic hapten antibody increases with a rise in temperature. In these interactions, ΔH° and ΔS° were calculated as positive one.View full abstractDownload PDF (723K) -
Naofumi Yoshioka, Tsuyoshi Yokoi, Masayuki Nata, Hitomi Meguro, Kaoru ...1991 Volume 35 Issue 4 Pages 297-301
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe cause of haptoglobin (Hp) deficiency in healthy adults is still unclear. Here, we picked up 7 Hp phenotype deficient samples out of 9, 711 sera from healthy donors by three successive serological procedures and then performed DNA analysis with Hp 2α specific probe. Hp gene was clearly detected in six individuals, which should be classified into temporary deficiency. One individual, however, was suggested to be a homozygous deletion of Hp gene cluster. The case (30-years-male) has a history of liver disease in his childhood and complains no clinical symptom caused by Hp deficiency. The analysis of chromosome on his leukocytes showed no deletion or abnormality. His mother was typed as Hp 2-2 with normal concentration of Hp. DNA linkage analysis with probes locating near Hp gene is under consideration.View full abstractDownload PDF (2988K) -
Shigenori Ikemoto, Emiko Fukui, Shuichi Tsuchida, Eiji Kajii, Sadahiko ...1991 Volume 35 Issue 4 Pages 303-306
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSIn this study on eight pairs of donors and recipients of bone marrow transplantation (BMT), broad range of gene markers at 16 gene loci, including 4 serum protein markers, 5 red cell enzyme markers detected by electrophoresis, and 7 salivary markers were evaluated before and 2, 4 and 6 months after BMT. As a result, 5 out of 16 gene loci of genetic markers in recipients were transformed into the donor type. BMT between family members may lead to transformation of gene markers, within a pattern compatible with family inheritance, and no genetic paradox will be found in later surveys of familial genetic relationships. However, in appearance of a phenotype incompatible with a blood relationship is possible after BMT from a non-blood-relative donor.View full abstractDownload PDF (1741K) -
Ikunosuke Sakurabayashi, Masahiro Daimon, Yoshikazu Hashimoto, Hatsuno ...1991 Volume 35 Issue 4 Pages 307-311
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSSerum protein fractionation by cellulose acetate electrophoresis is routinely used in clinical laboratories. Recently, various types of automated electrophoresis equipment are commercially available. Althogh we can easily take the percentage or absolute value of each serum protein fraction, there is no information for the presence of abnormal proteins (especially M-protein) or pathologic condition.
We attempted to develope the automated detecting programs for presence of M-protein and pathologic condition by the combination of computer and automated electrophoresis equipment. Five computer programs for the detection of M-protein and one program for the detection of pathologic condition were developed. According to these programs, more than 90% of M-proteins including small amout of various types of M-protein could be detected and 79% of the pathologic conditions were detected automatically.View full abstractDownload PDF (508K) -
Kohzoh Imai, Akira Yachi1991 Volume 35 Issue 4 Pages 313-321
Published: August 15, 1991
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSMonoclonal antibody (MoAb) HA 58 (IgG1) was established by immunizing interferon (IFN)-γ treated human colonic cancer BM 314 cells, to BALB/c mouse. Western blotting revealed that the MoAb HA 58 recognizes a 85kD molecule which is recognized by MoAb CL 207 to intercellular adhesion molecule-1 (ICAM-1). Immunodepletion experiment showed that each antibody reacts with the different epitope on the same ICAM-1 molecule. The level of cell surface ICAM-1 expression in cell lines of hematopoietic diseases was high in B-cell lines and especially high in myeloma cell lines. On the other hand, the level was low in T-cell lines and myeloid cell lines, except for ATL cell lines, which showed high level expression. In the investigation of ICAM-1 mRNA expression, the level was parallel to that of cell surface antigen.
We established ELISA system for detecting the ICAM-1 antigen by using two MoAbs, CL 207 and biotinylated HA 58. The incidence of positivity for ICAM-1 antigen in malignant diseases was higher than that in benign diseases or in healthy controls. The concentration of ICAM-1 antigen in sera of patients suffering from hematopoietic diseases was high in B-cell leukemia. In cases of B-cell type malignant lymphoma, the concentration was high in cases of advanced stage and/or in cases with leukemic change. As to T-cell malignancies, the concentration of serum ICAM-1 was high in cases of T-CLL, and ATL. The clinical features associated with high level serum ICAM-1 antigen were hepatosplenomegaly, invasion of tumor cells into organs, tumor formation and poor porgnosis.View full abstractDownload PDF (3241K)
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