SEIBUTSU BUTSURI KAGAKU
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
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Volume 37, Issue 5
Displaying 1-9 of 9 articles from this issue
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  • Instructive Lecture
    1993 Volume 37 Issue 5 Pages 255
    Published: October 15, 1993
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.37.255
    JOURNAL FREE ACCESS
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  • Workshop
    1993 Volume 37 Issue 5 Pages 257-261
    Published: October 15, 1993
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.37.257
    JOURNAL FREE ACCESS
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  • Review Article
    1993 Volume 37 Issue 5 Pages 262
    Published: October 15, 1993
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.37.262
    JOURNAL FREE ACCESS
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  • Symposium
    1993 Volume 37 Issue 5 Pages 263-269
    Published: October 15, 1993
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.37.263
    JOURNAL FREE ACCESS
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  • Plenary Session
    1993 Volume 37 Issue 5 Pages 270-318
    Published: October 15, 1993
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.37.270
    JOURNAL FREE ACCESS
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  • Distinction of pufferfish species by gel electrophoresis
    Ikuo Kazuta
    1993 Volume 37 Issue 5 Pages 319-323
    Published: October 15, 1993
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.37.319
    JOURNAL FREE ACCESS
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    Four species of the genus Lagocephalus pufferfish: L. gloveri (“kurosabafugu”), L. wheeleri (“shirosabafugu”), L. inermis (“kanafugu”) and L. lunaris (“dokusabafugu”) were discerned by the comparison of electrophoretic patterns of proteins and of L-Lactate dehydrogenase (LDH, EC 1.1.1.27) isozymes in skeletal muscle and vitreous humor. The proteins of skeletal muscle and vitreous humor were separated by polyacrylamide gel isoelectric focusing (PAG-IEF), and LDH isozymes were separated by agarose gel electrophoresis. The electrophoretograms of proteins of skeletal muscle and vitreous humor were significantly different from each other among the three species except for L. inermis. LDH zymograms in vitreous humor showed species specific patterns among the four species. Furthermore, a puncture of an eye ball of pufferfish enabled us to yield enough amount of sample to obtain the LDH zymograms not to give any damage to the fish. From these results, the comparison of LDH zymograms in vitreous humor is a useful method for distinction of these pufferfishes without making them unmarketable.
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  • Electrophoresis of bile acids in human bile
    Cellurose acetate membrane and agarose gel membrane
    Yuriko Mitsuhashi, Masaaki Nishimura, Masakazu Hirose, Makoto Inomata, ...
    1993 Volume 37 Issue 5 Pages 325-328
    Published: October 15, 1993
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.37.325
    JOURNAL FREE ACCESS
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    It was accomplished that free bile acids and conjugated bile acids were separated electrophoretically on the cellurose acetate and agarose gel membrane. Separated bile acids were stained by the 3α-hydroxysteroid dehydrogenase/diaphorase/dihormazane system. By this method, the human biles were analyzed and two purple bands were detected. The fast moving band was trihydroxy bile acid group (cholic acid). The slow moving band was dihydroxy bile acid group (deoxycholic and chenodeoxycholic acid). No Lithocholic acid was detected by this system in the human bile.
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  • Changes in serum proteins in experimental diabetic rats
    Tsuyoki Kadofuku, Tsuneo Sato
    1993 Volume 37 Issue 5 Pages 329-333
    Published: October 15, 1993
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.37.329
    JOURNAL FREE ACCESS
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    Changes in serum proteins in experimental diabetic rats caused by administration of streptozotocin (STZ) were investigated. Rats were administered 60mg/kg STZ and their serum proteins during 3-12 weeks were analyzed using two-dimensional polyacrylamide gel electrophoresis (PAGE) under non-denaturing conditions. Marked increase and decrease in amounts of several proteins were observed after STZ administration. These proteins almost returned to normal when the blood glucose level was controlled for 3 weeks at 100-200mg/dl with insulin. This suggest close relations between these proteins and diabetes.
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  • Erratum to : Studies on creatin kinase isoform analysis by means of electrophoresing reagentes for CK isozyme
    1993 Volume 37 Issue 5 Pages 335
    Published: 1993
    Released on J-STAGE: March 31, 2009
    DOIhttps://doi.org/10.2198/sbk.37.5_335
    JOURNAL FREE ACCESS
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