Carbohydrates are the least inappropriate group of compounds to be analyzed by capillary electrophoresis (CE), because normally they have no electric charge. However, they can be analyzed by this method by adding various devices. There are a number of methods for conversion of carbohydrates to positively or negatively charged derivatives, which can be analyzed directly by zone electrophoresis.
In-situ conversion with certain kinds of oxyacid ions, such as the borate ion, and metal ions, such as alkaline earth metal ions, also form ionic complexes from carbohydrates and derivatives. Furthermore, introduction of hydrophobic groups allows separation based on solubilization of the derivatives into ionic micells, thus permitting micellar electrokinetic chromatography. This paper describes not only such theoretical aspect of CE but its application to the analysis of oligosaccharides in glycoproteins. It gives a number of examples of successful application.
抄録全体を表示