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Volume 40 (1996)
- Issue 6 Pages 289-
- Issue 5 Pages 229-
- Issue 4 Pages 175-
- Issue 3 Pages 95-
- Issue 2 Pages 47-
- Issue 1 Pages 1-
Volume 40, Issue 4
Displaying 1-7 of 7 articles from this issue
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Toshiko Ueda, Tsuyoshi Maekawa, Daikai Sadamitsu, Ryosuke Tsuruta, Kaz ...1996 Volume 40 Issue 4 Pages 175-181
Published: August 15, 1996
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSThe determination of nitrite and nitrate in human blood plasma by capillary electrophoresis (CE) is described. Electrophoresis was carried out in a 60cm long, 75μm wide fused-silica capillary, using 750mM sodium chloride containing 5% NICE-Pak OFM Anion-BT as a running buffer, at a potential of 20kV, with in-column UV detection at 214nm. Under these conditions, the two anions migrated in the order of nitrite and nitrate with complete resolution. The linearity, in the range of 0.1-50mg/l, was described by the equation y=1425+8261x r2=0.999 (x: nitrite concentrations; y: peak area), and y=1417+8416x r2=0.999 (x: nitrate; y: peak area). The coefficients of variation (CVs) in nitrite and nitrate were lower than 8% and 5%, respectively. The recovery was 97-114%. Forty-one samples of ultrafiltered blood were analyzed for nitrite and nitrate using CE in healthy volunteers. The concentrations of nitrite and nitrate were 0.15±0.07mg/l and 3.2±1.6mg/l (mean±SD), respectively. Plasma nitrite and nitrate were measured in three pathologic states. In septic patient, plasma nitrate was increased when peripheral leukocytes increased. In CaS intoxicated patient who was treated with sodium nitrite, plasma nitrite increased immediately followed by the increase of plasma nitrate. In the case of subarachnoid hemorrhage, plasma nitrate tended to be decreased from 4th day through 11th after subarachnoid hemorrhage. This method is useful to treat the critical care patients in the cases of shock, intoxication, and administration of nitrite.View full abstractDownload PDF (759K) -
Michinari Yokohama, Taisuke Yamazaki, Toshihiro Watanabe, Yoshirou Ish ...1996 Volume 40 Issue 4 Pages 183-186
Published: August 15, 1996
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSFrom the diversity of size heterogeneity among equine transferrin (Tf) types and quantitative variants between T+ and T- components composed of Tf types, the Tf types could be classified into three groups as Tf-D·F·H, Tf-O·R and Tf-X subgroups. To certify furthermore the results, the equine Tf types were subgrouped from peptide cleavage patterns produced by peptide mapping using four proteases (α-chymotrysin, V8 protease, papain and endoprotease Asp-N). The results obtained are as follows: 1) When Tf·D, F and H types were treated by the four proteases, these three Tf types had almost the same peptide cleavage patterns. Although there were respectively some different band patterns between each peptide cleaved in Tf·O and R types, they had more mutually similar points than those of the Tf-D·F·H subgroup. This means that Tf·O and R types may be mutually specialized, but it was guessed that they can be basically classified into the same subgroup in view of having many of the same peptide cleavage components. The four peptide patterns which were cleaved donkey's Tf components, were each very similar with those of equine Tf·O and R types. 2) T+ and T- components of Tf·D and R types were each cleaved by V8 protease and α-chymotrypsin, and both components of the Tf·D type showed almost same peptide pattern, and there were distinct differences when the two components of the Tf·R type were digested by the latter protease. From analysis by peptide mapping, Tf·O and R types might be newly subgrouped, but it was again recognized that the peptide structures of the Tf·O and R types were each distinctly different from those of the Tf-D·F·H subgroup. Accordingly, the equine Tf types could be classified into two groups as Tf-D·F·H and Tf-O·R types by peptide mapping. Then the T+ and T- components in the Tf·R type had basically different peptide structure, respectively.View full abstractDownload PDF (3832K) -
Toshio Okazaki, Nobuyuki Kadohno, Tatsuo Nagai, Takashi Kanno1996 Volume 40 Issue 4 Pages 187-192
Published: August 15, 1996
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSA residual IgG line is frequently observed inside the monoclonal IgG line on immunoelectrophoresis of IgG myeloma serum in reaction with anti-whole human serum. We found that the residual IgG line was observed exclusively in serum from IgG-λ type M-proteinemia when a goat antiserum to whole human serum was employed. However, with a horse antiserum to whole human serum, the residual IgG line was observed in sera from both the IgG-λ and κ type M-proteinemias. These phenomena led us to an idea that the appearance of the residual IgG line resulted from reaction with anti-κ or λ antibodies present in the anti-whole human serum antisera. Investigations of sera from a number of IgG M-proteinemia by anti-κ and λ antisera, and absorption tests with partially purified BJP of both types indicated that the occurrence of the residual IgG line is due to the reaction of polyclonal IgG with anti-κ or λ chain antibody present in the anti-whole human serum.View full abstractDownload PDF (5511K) -
Atsushi Hiraoka, Teruyo Arato, Itaru Tominaga1996 Volume 40 Issue 4 Pages 193-197
Published: August 15, 1996
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSCapillary zone electrophoresis (CZE) was applied to the separation and determination of two major monoamine metabolites, 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA) in cerebrospinal fluid (CSF) from patients with neuropsychiatric disorders. Ethyl acetate extracts of acidified CSF were analyzed by CZE with ultraviolet absorbance detection (UVD). The peaks of 5-HIAA and HVA were clearly detected on the electropherograms, and their levels in the CSF samples were determined on the basis of the peak-area ratios relative to p-methoxybenzoate spiked as an internal standard. The results showed that the CSF levels of 5-HIAA and HVA reduced in patients with some diseases, such as Parkinson's disease, Altzheimer's disease, depressive illness, etc., supporting the conclusions obtained earlier by high-performance liquid chromatography (HPLC). The present CZE-UVD system, which consumes only a far smaller amout of sample solutions to be injected than that required in HPLC-UVD, seems to be useful as an aid in the biochemical diagnosis of neuropsychiatric disorders, especially in distinguishing dementia associated with central nervous system-degenerative diseases (both of the 5-HIAA and HVA levels in CSF are reduced) from that with cerebrovascular diseases (neither the CSF 5-HIAA nor HVA levels are changed).View full abstractDownload PDF (603K) -
Yuhsaku Kanoh, Keiko Ichikawa, Satoshi Jimbo, Yoshihiko Shimura, Kazuo ...1996 Volume 40 Issue 4 Pages 199-206
Published: August 15, 1996
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSWe measured concentration of some proteins such as interleukin-6 (IL-6), albumin (Alb), CRP and α2 macroglobulin (α2M) in both cerebrospinal fluid (CSF) and serum of patients with meningitis in order to evaluate the damage of blood-cerebrospinal fluid barrier (BCB). The permeability of the barrier was large for small molecular protein as in the order of Alb, CRP and α2M. CRP level in CSF was proportional to CRP level in serum. Extremely high α2M level in CSF was found only in patients whose barriers were damaged. α2M level in CSF was well correlated to the ratio of (large protein)/(small protein) determined by HPLC, to the α2M index ([α2M]CSF/[α2M]serum)/([Alb]CSF/[Alb]serum), and to cell counts in CSF. CRP concetration in CSF in bacterial meningitis (average 74.7μg/dl) was much higher than in viral meningitis (4.1μg/dl). However, CRP in CSF increased also in bacterial infection without meningitis (45.3μg/dl). Therefore, the determination of CRP and α2M in CSF will be useful for differential diagnosis among bacterial meningitis, bacterial infection without meningitis and viral meningitis. IL-6 in CSF increased in acute phase of meningitis, and reflected the activity of meningitis.View full abstractDownload PDF (2601K) -
Keiko Morita, Naomi Takaoka, Chikara Kurata, Chikara Kubota, Yasuyuki ...1996 Volume 40 Issue 4 Pages 207-210
Published: August 15, 1996
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (4023K) -
Naoyuki Nakata, Tatsuo Tozawa1996 Volume 40 Issue 4 Pages 211-214
Published: August 15, 1996
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (3296K)
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