N-linked and O-linked oligosaccharides from glycoproteins in human pathological kidneys, dissected from patients suffering from renal cell carcinoma, were analyzed by fluorophore-assisted carbohydrate electrophoresis (FACE) on polyacrylamide slab gels. The resulting electrophoretic profiles of normal and cancerous regions were compared. Renal cell carcinoma was found to contain three bands of N-linked and three bands of O-linked oligosaccharides with strong intensity in fluorescence that were hardly detected in the normal regions. The apparent electrophoretic mobilities of these bands, expressed by DP (degree of polymerization of glucose) value, were measured to be DP=3.0, 4.0 and 5.4 for N-linked oligosaccharide, and DP=2.0, 3.0 and 3.5 for O-linked oligosaccharide. The profiles obtained from 8 pathological specimens revealed that two bands of N-linked oligosaccharide with DP=3.0 and 4.0, and one band of O-linked oligosaccharide with DP=3.0 were detectable without exception, suggesting that these oligosaccharides were specific to renal cell carcinoma.
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