SEIBUTSU BUTSURI KAGAKU
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
Volume 41, Issue 4
Displaying 1-7 of 7 articles from this issue
  • Tosifusa Toda
    1997 Volume 41 Issue 4 Pages 169-172
    Published: August 15, 1997
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
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  • The importance of the electrokinetic properties of magnetic polymer carriers
    W. Schütt, C. Grüttner, J. Teller, F. Westphal, U. Häfe ...
    1997 Volume 41 Issue 4 Pages 173-178
    Published: August 15, 1997
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    Radionuclide therapy depends for success on the critical relationship between the amount of radionuclide in the target tissue and in critical normal tissue. The successful implementation of radioimmunotherapy for treatment of cancer has proven to be considerably more difficult than initially anticipated. Several groups have tried the magnetic guidance of antiplastic and antibacterial agents as well as X-ray-and MRI-contrast substances by means of magnetic particles. We propose to use magnetic nanospheres for the directed delivery of radionuclide to the tumor after intravenous administration of the biodegradable colloidal suspension. Using carriers of this size range the circulation time in the blood, the biodistribution and more or less the specific targeting are determined by uniformity of size, chemical composition, surface modification and the electric surface charge. The electrophoretic mobility is an important parameter for the prediction of the usefulness of the prepared particle, modified by chemical and biological molecules.
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  • Kiyohito Shimura, Ken-ichi Kasai
    1997 Volume 41 Issue 4 Pages 179-185
    Published: August 15, 1997
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    Affinophoresis is a type of affinity electrophoresis in which an affinophore, a conjugate of an affinity ligand and a multiply charged soluble matrix, causes a mobility change in proteins that have specific affinity for the ligand. This technique enables, not only the separation of a specific molecule, but also the analysis of specific interaction between biomolecules. The affinity constant between fluorescence-labeled pea lectin and affinophores bearing mannoside as an affinity ligand, was determined by capillary electrophoresis using laser-induced-fluorescence detection. Although the labeling of the protein caused microheterogeneity as evidenced by electrophoretic mobility, the affinity constant determined for the main peak in the electropherogram of the labeled lectin agreed with those obtained for unlabeled lectin by the same type of analysis using ultraviolet-absorption detection. As a result of the use of the fluorescence detection, the consumption of the protein sample is greatly reduced to several pg per run.
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  • Kiyotaka Fujita, Ikunosuke Sakurabayashi, Mutsuko Kusanagi, Takashi Yo ...
    1997 Volume 41 Issue 4 Pages 187-192
    Published: August 15, 1997
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    The reaction of a human IgM-κ type M-protein with cellulose acetate (Separax) membrane is described. When a membrane consisting of diacetylcellulose and triacetylcellulose (1:1) was used, the M-protein showed a strong reactivity with this membrane. However, this strong reactivity was absent when a Separax membrane containing hydroxypropylcellulose an electro-osmosis removal reagent was used. Likewise, alkaline saponification of the membrane, changing acetyl groups into hydroxyl groups, did not show reactivity with the M-protein. A patient studied had no history of hemodialysis using a cellulose membrane. Therefore, the possibility exists that the reaction between acetyl groups of the Separax membrane and the patient's M-protein is due to immunological cross reactivity.
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  • IV. Anionic isotacophoresis of ampholytes using a common component which migrates faster than the leading component
    Kazuo Shimao
    1997 Volume 41 Issue 4 Pages 193-197
    Published: August 15, 1997
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    Our computer simulation procedure was applied to an anionic isotachophoretic system of ampholytes in which a component having a migration velocity faster than the leading component of the system was added to the catholyte. After a certain time of electrophoresis depending on the initial condition, isotachophoretic migration resulted even in the presence of the faster component in the catholyte. The isotachophoretic concentration of the ampholytes and the potential gradient were lower in the presence than those in the absence of the faster component. The lower potential gradient is preferable from the standpoint of Joule's heat so that the proposed isotachophoretic system seems to be useful for slab gel isotachophoretic experiments where thinning of the gel occurrs in the region of high potential gradient.
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  • Tatsuo Tozawa
    1997 Volume 41 Issue 4 Pages 199-205
    Published: August 15, 1997
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
    We examined the prevalence of amylase-linked immunoglobulin complexes, known as macroamylase, in the sera of 146 patients with ulcerative colitis (UC). Six of them were found to have this complex. Combining this group with the group of 59 patients with UC examined previously in a similar fashion, provided a prevalence rate of 7 in 205 (3.4%). Of the 7 patients the binding immunoglobulin was IgG in 4, IgA in 2 and both in the remaining patient. This distribution of the immunoglobulins contrasts strikingly with a preponderance of IgA in patients with a variety of diseases. The results make definite the association between UC and circulating enzyme-linked IgG complex, and provide support for the hypothesis that autoimmune mechanisms are involved in the pathogenesis of UC.
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  • Chieko Sugimura, Kiyotaka Fujita, Mutsuko Kusanagi, Norikazu Suzuki, I ...
    1997 Volume 41 Issue 4 Pages 207-210
    Published: August 15, 1997
    Released on J-STAGE: March 31, 2009
    JOURNAL FREE ACCESS
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