巻号一覧
後続誌
42 巻, 4 号
選択された号の論文の11件中1～11を表示しています
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現状と将来の展望今井 浩三1998 年 42 巻 4 号 p. 217-223
発行日: 1998/12/15
公開日: 2009/03/31
ジャーナル フリーGenetic analysis for tumor cells has recently been advanced due to the development of the new biotechnology. In this review article, recent advancement of the genetic instability in digestive tract cancers and hypermethylation of the promoter region of the gene was reviewed. The new data from our laboratory concerning these points was presented in addition to the detailed description of the methodology.抄録全体を表示PDF形式でダウンロード (2350K) -
三善 英知, 谷口 直之1998 年 42 巻 4 号 p. 225-231
発行日: 1998/12/15
公開日: 2009/03/31
ジャーナル フリーα1-6 Fucosyltransferase (α1-6 FucT) catalyzes the transfer of fucose to innermost GlcNAc in N-glycans. This structure on α-fetoprotein has been clinically used for early diagnosis of hepatocellular carcinoma. In this study, expression of α1-6 FucT was investigated in rat and human hepatocarcinogenesis and biological significance of α1-6 FucT on hepatoma was investigated in terms of metastasis. While α1-6 FucT was scarcely detected in normal liver, it was enhanced in LEC rat hepatoma, but not in their surrounding tissues. In contrast, high expression of α1-6 FucT mRNA was observed in human liver cirrhosis tissues as well as in hepatoma tissues. We established human hepatoma cell lines which express high levels of α1-6 FucT by transfection of α1-6 FucT gene and investigated intrahepatic metastasis after splenic injection to athymic mice. Tumor formation in the liver was dramatically suppressed in α1-6 FucT transfectants. While there were no differences of cell invasiveness and cytotoxicity to lymphocytes, cell adhesion to nonparenchymal liver cells in culture was significantly inhibited in α1-6 FucT transfectants as compared to those of controls. Attachment of α1-6 FucT transfectants to a fibronectin or laminin coated dish was decreased compared to controls. Two-dimensional electrophoresis followed by lectin blot showed certain glycoproteins were α1-6 fucosylated and might be linked to suppression of intrahepatic metastasis. Collectively, although α1-6 FucT was enhanced in rat and human hepatocarcinogenesis, overexpression of α1-6 FucT suppressed intrahepatic metastasis of hepatoma.抄録全体を表示PDF形式でダウンロード (5980K) -
斎藤 督1998 年 42 巻 4 号 p. 233-237
発行日: 1998/12/15
公開日: 2009/03/31
ジャーナル フリーAllelic deletions of chromosome 6q that occur frequently in ovarian cancers imply the presence of a putative tumor suppressor gene in this chromosomal vicinity. We analyzed DNA from 32 patients with ovarian carcinomas for loss of heterozygosity at loci on the distal portion of chromosome 6q and constructed a detailed deletion map. The map indicated a commonly deleted region between loci D 6S 149 (defined by CI6-24) and A 2, which are estimated to be 300kb apart on the basis of our cosmid contig map. DNA sequencing in the region disclosed the presence of AF-6, a gene that had been identified as the ALL-1 fusion partner involved in acute myeloid leukemias with t (6; 11) (q27; q23) translocations. Subsequent screening of the AF-6 gene in ovarian carcinomas revealed no mutations.抄録全体を表示PDF形式でダウンロード (538K) -
井上 貴博, 大川 清1998 年 42 巻 4 号 p. 239-243
発行日: 1998/12/15
公開日: 2009/03/31
ジャーナル フリーアルコール性肝障害において出現する血清CDTの糖鎖構造について, レクチン親和電気泳動とSDS-PAGEより分析した. CDTはConAおよびDSAとの結合性が消失し, さらにN-グリコシダーゼF処理のTfと分子量が一致していたことから, シアル酸だけでなく糖鎖そのものが欠損しているTfであることが明らかになった. このような現象はTf以外のAGPでも認められ, 先天性疾患である carbohydrate deficient glycoprotein syndrome type Iと非常に類似していた.抄録全体を表示PDF形式でダウンロード (3697K) -
中島 孝則, 河井 信一郎, 松永 俊之, 穗苅 茂, 那須 英和, 菰田 二一1998 年 42 巻 4 号 p. 245-249
発行日: 1998/12/15
公開日: 2009/03/31
ジャーナル フリーWe obtained a monoclonal antibody 9F 5-3 a against oxidative high-density lipoprotein (HDL) modified with CuSO4. In addition, we have examined the epitope of 9F 5-3 a and the localization of oxidized HDL (oxHDL) in atheromatous plaque. The 9F 5-3 a was reacted strongly with oxHDL and to a lesser degree with oxidized low-density lipoprotein (oxLDL) and native LDL. No reactivity was found with native HDL. Furthermore, the cross-linkage of apolipoprotein A-I and/or apolipoprotein A-II was accelerated by HDL oxidation, and the crosslinked apolipoprotein forms (32, 60, 90, 120 and 200-kDas) were selectively recognized by the 9F 5-3 a antibody. OxHDL particles reacting with the 9F 5-3 a antibody were localized histochemically in the luminal side of intima of atheromatous plaques in human abdominal aortae. Using a ligand receptor assay for oxHDL, a major 130-kDa band was detected in cultured human aortic endothelial cells. These results suggested that oxHDL particles and its putative binding protein were present in atheromatous plaques and endothelial cells, respectively.抄録全体を表示PDF形式でダウンロード (5441K) -
伊東 文生, 日野田 裕治1998 年 42 巻 4 号 p. 251-255
発行日: 1998/12/15
公開日: 2009/03/31
ジャーナル フリー近年, 遺伝子の解析技術は日進月歩であり, 遺伝子の一次構造の解析に始まり, 分子の機能の解析への応用, さらには遺伝子異常検出の臨床医学への応用も進められている. 本稿では, PCR法を基礎とした遺伝子解析技術の応用について概説した. 癌はすでに遺伝子異常の蓄積によるいわゆる多段階発癌の機構で発症するものと考えられている. すでに, 多種類の癌疾患において異常を呈する遺伝子が同定されており, 一部はその癌化における役割も明らかになっている. しかし, これまでの報告を総合しても, 癌をおこしうる遺伝子のうちごく一部が解明されているにすぎないのが現状と思われる. 今後, 新たな遺伝子の異常を知るためには, 癌において特異的に異常を呈する遺伝子の同定法の開発が必要になる. そこで, 近年発達したPCR法を基礎に用いる2つの遺伝子解析法 (ゲノム遺伝子の異常の検出法としてRDA法, mRNAの差の検出法として Differential display 法) について, 実際にこれらの方法を胃癌に応用した例についてRDA法を中心としてその知見を述べ, 新たな遺伝子異常検出法としての可能性を示す.抄録全体を表示PDF形式でダウンロード (3375K) -
縄田 修吾, 加藤 紘, 中村 和行1998 年 42 巻 4 号 p. 257-263
発行日: 1998/12/15
公開日: 2009/03/31
ジャーナル フリーRecently, tumor markers, which have been developed for the diagnosis and evaluation for cancer, are regarded as new key tools to study the malignant behavior of cancer, and therefore, much attension has been focused on their biological functions. Squamous cell carcinoma (SCC) antigen is a tumor marker of SCC arising in various sites. We have investigated the biological function and heterogeneity of this tumor marker by electrophoretic methods. In this paper, we report that SCC antigen belongs to the inhibitory type of serine protease inhibitor family, indicating that SCC antigen may affect malignant behavior such as tumor invasion. We also describe the different pattern of SCC antigen by two-dimensional electrophoresis in normal and cancer tissues, and indicate that the heterogeneity of SCC antigen results mainly from the presence of two homologous genes, SCCA 1 and SCCA 2 gene.抄録全体を表示PDF形式でダウンロード (5743K) -
Corinne E. M. Olesen, Jennifer Mosier, Chris S. Martin, John C. Voyta, ...1998 年 42 巻 4 号 p. 265-279
発行日: 1998/12/15
公開日: 2009/03/31
ジャーナル フリーThe use of 1, 2-dioxetane chemiluminescent enzyme substrates, including AMPPD®, CSPD®, CDP® and CDP-Star® for alkaline phosphatase and Galacton-Star® substrate for β-galactosidase, provides highly sensitive detection for numerous immunoassay and nucleic acid detection formats. Enzyme cleavage of the 1, 2-dioxetane substrate generates a metastable anion intermediate that decomposes with the concomitant emission of light. Light emission exhibits glow kinetics, enabling the use of multiple imaging platforms for signal detection, including film-based, luminometers, low-light sensitive camera and phosphor screen instrumentation systems. Applications include both membrane-based immunodetection of proteins and nucleic acid blot hybridization, and solution-based immunoassays and nucleic acid capture/hybridization assays performed in a microwell plate.抄録全体を表示PDF形式でダウンロード (4446K) -
芦原 義弘, 磯村 光男1998 年 42 巻 4 号 p. 281-285
発行日: 1998/12/15
公開日: 2009/03/31
ジャーナル フリーChemiluminescent immunoassays have commonly been used in clinical laboratories because of their high sensitivity and wide dynamic range. We employed highly sensitive and stable 1, 2-dioxetane substrates, AMPPDTM and CSPDTM for alkaline phosphatase (ALP) and applied chemiluminescent enzyme immunoassay (CLEIA) for the detection of tumor markers and antibodies to infectious viruses. Chemiluminescent enzyme immunoassay using AMPPD as ALP substrate showed S/N values of more than 80 times those of CLEIA using fluorescent or colorimetric substrate. The chemiluminescent reaction using CSPD reached a plateau at 5 minutes. We observed a higher chemiluminescent signal by energy transfer using several fluorescent reagents in the presence of AMPPD and the enhancer BDMQ. Gelatin-coated ferrite particles as a solid phase showed a high dispersion floating property and very low background noise. We established an extremely sensitive CLEIA for IL-6 in which the detection limit was 0.1pg/ml (zero+2 SD). We also applied gelatin solid phase to establish a sensitive CLEIA by reducing background noise through the release of an immne complex consisting of solid antibody, antigen and labeled antibody from ferrite particles using gelatinase. In this method, 7.5 times higher S/N than conventional CLEIA was obtained.抄録全体を表示PDF形式でダウンロード (590K) -
久保野 勝男, 櫻林 郁之介1998 年 42 巻 4 号 p. 287-293
発行日: 1998/12/15
公開日: 2009/03/31
ジャーナル フリーLipoprotein(a) [Lp(a)] 測定に関する標準化については, IFCCのLp(a)標準化に関するワーキンググループ (IFCC Working Group on Lp(a)) や日本臨床病理学会標準化委員会のアドホックLp(a)標準小委員会が活動を行っている. 現在までにIFCC Lp(a)WGでは, 8種の Proposed Reference Material 候補品全般の各種性能評価比較を実施し, その中からPRM-2B (Behring 社) を最終的なPRMとして選択した. さらに今後は Primary Reference Material からPRM-2Bへの値付けと重量表示から mole 表示への変更を含めた国際的 Harmonization を目指すこととしている. 今後1年以内に検討を終了し, WGとしての結論をIFCCから最終的にはWHOに答申していくものと考えられる. 今までの臨床的評価に加えて, mole レベルでのデータ蓄積がされることによって, 新たな評価が得られる可能性を持っており, 標準化以降の今後の臨床的検討に期待したい.抄録全体を表示PDF形式でダウンロード (7577K) -
小川 善資, 伊藤 啓1998 年 42 巻 4 号 p. 295-298
発行日: 1998/12/15
公開日: 2009/03/31
ジャーナル フリーOn the enzyme committee of Japan Society of Clinical Chemistry, the studies of enzyme assay for the recommendation methods was started from 1975. Already, recommendation methods of aspartate aminotransferase, alanine aminotransferase, creatine kinase, alkaline phosphatase, lactate dehydrogenase and γ-glutamyltransferase were published. And new, two kinds of enzymes, amylase and cholinesterase were in progress to discuss for the consensus.抄録全体を表示PDF形式でダウンロード (458K)
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