生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
43 巻, 4 号
選択された号の論文の7件中1~7を表示しています
  • 橋本 信也
    1999 年 43 巻 4 号 p. 199-201
    発行日: 1999/12/15
    公開日: 2009/03/31
    ジャーナル フリー
  • Johann Bauer, Wolfgang Schütt, Domagoj Sabolovic, 橋本 信也
    1999 年 43 巻 4 号 p. 219-223
    発行日: 1999/12/15
    公開日: 2009/03/31
    ジャーナル フリー
    At ICES '99 particular emphasis was laid on electrophoresis of cells and particles dedicating a complete session to this topic. Challenged by this organizational strategy of Prof. Hashimoto 16 highly interesting abstracts had been submitted, of which 8 were presented orally. All of them showed that analytical as well as preparative electrophoresis of cells and particles have already reached a very high level of performance. Some possibilities of improving resolution were still proposed regarding analytical (3 abstracts) as well as preparative (2 abstracts) carrier free electrophoresis devices. All the other presentations, however, clearly demonstrated which tremendous impact on research and even on commercial production electrophoresis of cells and particles currently has. Outstanding results in chemical, biochemical and medical research were explained which would not have been obtained without carrier free electrophoresis technologies.
  • 寺部 茂, Joselito P. Quirino
    1999 年 43 巻 4 号 p. 225-231
    発行日: 1999/12/15
    公開日: 2009/03/31
    ジャーナル フリー
    Micellar electrokinetic chromatography (MEKC) as well as other capillary electrophoretic modes suffers from low concentration sensitivity due to low sample volume and limited pathlength for on-capillary photometric detection. On-line sample concentration techniques have been developed by simple manipulation of sample and background solution constituents without alteration of MEKC instrumentation, which are sample stacking and sweeping. These focusing techniques provide from 10 to more than 5000-fold improvement of the detector response. The enhancements are equivalent to 1-3 orders of magnitude improvement in the limit of detection. They are simple and are easily transferable technologies.
  • 菰田 二一
    1999 年 43 巻 4 号 p. 233-241
    発行日: 1999/12/15
    公開日: 2009/03/31
    ジャーナル フリー
  • 戸田 年総
    1999 年 43 巻 4 号 p. 243-244
    発行日: 1999/12/15
    公開日: 2009/03/31
    ジャーナル フリー
  • V. Computer simulation of sodium dodecyl sulfate-polyacrylamide gel electrophoresis
    島尾 和男
    1999 年 43 巻 4 号 p. 245-250
    発行日: 1999/12/15
    公開日: 2009/03/31
    ジャーナル フリー
    The behaviors of all the components of glycine and tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis system in the course of separation of polypeptide-dodecyl sulfate complexes are investigated by computer simulation. In both of the systems, the leading chloride ion zone is followed by concentrated dodecyl sulfate zone, the length of which gradually increases with the time of electrophoresis. Under the postulated conditions and the values of physico-chemical parameters of the components of the systems, almost all the polypeptides migrate as the dodecyl sulfate-polypeptide complexes. These complexes are stacked between the dodecyl sulfate zone and the terminating glycine or tricine zone when they reach the separation gel. The high mobility complexes are not overtaken by the terminating glycine in the separation gel but stacked between the dodecyl sulfate and the glycine zone, while, all the complexes are overtaken in the separation gel by the terminating tricine and separated in the separation gel. Thus, the reason for the usefulness of tricine for the separation of the low molecular complexes is clarified by the computer simulation.
  • 藤原 淳子, 李 栄成, 佐藤 淳子, 吉田 克己, 佐々木 毅, 舩渡 忠男
    1999 年 43 巻 4 号 p. 251-254
    発行日: 1999/12/15
    公開日: 2009/03/31
    ジャーナル フリー
    A non-radioisotopic PCR-SSCP method using fluorescently labeled primers was developed for use in a clinical setting. In order to establish PCR-SSCP method for the clinical use, we developed a non-radioisotopic PCR-SSCP method using fluorescence-labeled primers. Electrophoresis in buffer containing with MgCl2 provided good separation of the bands and use of an image analyser gave more sensitive detection than conventional method. The mutations of K-ras gene and androgen receptor gene were confirmed by DNA sequencing analysis of the known mutations of K-ras gene and the unknown mutations of androgen receptor gene. The fluorescence-mediated PCR-SSCP system made it possible to detect mutations more rapidly and with more sensitivity than conventional PCR. Thus, the improved PCR-SSCP method will be useful for the clinical screening of unknown mutatios.
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