生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
45 巻, 3 号
選択された号の論文の3件中1~3を表示しています
  • 山崎 泰助, 櫻井 進, 河野 稔
    2001 年 45 巻 3 号 p. 171-175
    発行日: 2001/09/15
    公開日: 2009/03/31
    ジャーナル フリー
    6週齢のDBA/1 J雌マウスに少量のウシ由来II型コラーゲン (bIIC) を接種後, 関節腫脹が認められないことを確認した3週後にスーパー抗原活性をもつ黄色ブドウ球菌表皮剥脱毒素血清型A (ETA) を接種することによって関節炎の誘導実験を行った. その結果, 1群5匹中, 3~4匹のマウスにおいて, 発赤, 指関節を中心とした関節腫脹を伴う関節炎が誘導された. また腫脹関節では, 骨, 軟骨破壊および滑膜細胞の増殖に関与する炎症性サイトカインとしてのIL-1β, IL-6, TNF-αおよびIFN-γの過剰発現が確認された. 以上の結果, 黄色ブドウ球菌が産生する外毒素の1つであるETAは, そのスーパー抗原活性によりサイトカインを過剰発現させ, 関節炎を誘導, 増悪させることが示唆された.
  • 小山 岩雄, 原田 剛, 松永 俊之, 穂苅 茂, 津村 真由美, 片山 博雄, 菰田 二一
    2001 年 45 巻 3 号 p. 177-183
    発行日: 2001/09/15
    公開日: 2009/03/31
    ジャーナル フリー
    The characteristics of alkaline phosphatase (AP) and nitric oxide synthase (NOS) in the lung were investigated using rats challenged with lipopolysaccharide (LPS). Lung homogenates and alveolar lavage fluid were prepared from rats after an intratracheal instillation of 20mg/kg body weight of LPS. AP activities in homogenates were elevated up to 2hr and had recovered to the control level by 6hr. In contrast, those in lavage fluid were significantly decreased after 30min and recovered to the control level by 6hr. Although APs in normal lavage fluid were of the membranous-type in addition to the soluble-type, APs after LPS-challenge were soluble-type only. The 75kDa AP molecule in the homogenate at 2hr after LPS-challenge was increased, in comparison with the control, as was the level of AP activity. In contrast, there was no difference in AP protein level in lavage fluid between the control and LPS-challenged rats. Inducible NOS (iNOS) was obviously induced in lung homogenates by LPS-challenge. Moreover, proteins containing nitrotyrosine as a marker of peroxynitrite were identifiable by Western blot analysis in lavage fluid from rats challenged with LPS. These results indicate that LPS-challenge directly affects the expression and secretion of APs from type II pneumocytes.
  • 久保 兼信, 森嶋 祥之
    2001 年 45 巻 3 号 p. 185-191
    発行日: 2001/09/15
    公開日: 2009/03/31
    ジャーナル フリー
    Human serum proteins including myeloma (M) proteins were analyzed by capillary zone electrophoresis (CZE) using a linear polyacrylamide-coated capillary at pH 7.4. Each of the M proteins, IgG-κ, IgA-κ, and IgA-λ, which were located on the β zone on the cellulose acetate electrophoresis (CAE), was resolved into a group of peaks with a unique profile in a wide area following the transferrin (TF) peak. While the IgG-κ showed a series of narrow peaks, IgA-κ and IgA-λ showed a wide peak with many successive spikes. On the other hand, the increased polyclonal IgA-κ and IgG-κ, which were located on the β zone on the CAE, showed only a broad peak around the TF peak. Another IgG-κ M protein, which was located on the γ zone on the CAE, did not appear on the CZE at pH 7.4, but did appear with several peaks by increasing the pH to 9.0. No peaks were observed in the area following the TF peak when healthy serum proteins including immunoglobulins (Igs) were analyzed at both pHs. The coexistence of albumin did not affect the detection of monoclonal and polyclonal Igs. These results demonstrate that the CZE system is capable of distinguishing IgG M proteins from IgA M proteins, and monoclonal Igs from polyclonal ones. Furthermore, it was found that the CZE is useful for discriminating between isoforms of IgG M proteins.
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