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Volume 49 (2005)
- Issue 4 Pages 97-
- Issue 3 Pages 63-
- Issue 2 Pages 31-
- Issue 1 Pages 1-
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Volume 49, Issue 2
Displaying 1-4 of 4 articles from this issue
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Yoshihisa Itoh2005 Volume 49 Issue 2 Pages 31-35
Published: June 15, 2005
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSUrine should be a most valuable routine sample in clinical laboratory. Laboratory data in urine are, however, more or less influenced by many known or unknown, pre-renal, renal and post-renal factors, thus giving the measured value complex and equivocal. In relevance with physicochemical properties and structure of individual proteins, the present author reviewed the factors and their effects on laboratory data in the framework of quality assurance system. Taking properties of individual proteins into considerations we must need to strengthen their clinical significance continuously by investigating and eliminating what are affected by the participating factors.View full abstractDownload PDF (2549K) -
2005 Volume 49 Issue 2 Pages 37-46
Published: June 15, 2005
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSDownload PDF (9765K) -
Yasuhiro Kuramitsu2005 Volume 49 Issue 2 Pages 47-50
Published: June 15, 2005
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSElectrophoresis techniques have been used not only for scientific research, but also for scientific education. At Yamaguchi University School of Medicine, our students have several chances to learn electrophoresis techniques. Second year medical students are required to learn biochemical laboratory techniques for doing future research. They learn agarose gel electrophoresis for DNA analysis and cellulose acetate membrane electrophoresis for serum separation. This university offers a unique program for education, called “self-developing course”. During this four month course, students can belong to whatever department or institute they wish. Some students choose our laboratory where they perform basic or advanced experiments that they plan by themselves. Mainly they investigate proteomics or signal transduction. For these experiments, electrophoresis techniques, such as two-dimensional gel electrophoresis (2DE), Western blotting and 2D-Western blotting, are indispensable. In addition, all graduate students are instructed in electrophoresis techniques.View full abstractDownload PDF (3679K) -
Jun Suzuki2005 Volume 49 Issue 2 Pages 51-54
Published: June 15, 2005
Released on J-STAGE: March 31, 2009
JOURNAL FREE ACCESSIn a “health and environmental sciences” program, college students study not only health, but also methods for improving and developing a comfortable life environment by focusing on the relationship between humans and the environment. Students are specifically taught to acquire the knowledge necessary for overall understanding and analysis of various environmental problems, which have expanded from a local to worldwide scale.
The authors have class the environmental toxicology, biochemistry, and clinical applications of electrophoresis.
Assessment of the negative effects of chemical substances has recently become a subject of discussion in the field of environmental health. Assessments of chemical substances have been carried out using pathology and plasma analysis. Two-dimensional-electrophoresis (2D-EP) is one of the most powerful separation techniques for plasma analysis used in the field of environmental health. We succeeded in developing a rapid determination method using micro two-dimensional polyacrylamide gel electrophoresis (M2D-PAGE) that requires approximately 30 minutes to complete. With the use of Rapid 2D-PAGE, it is possible to assess the effects of environmental toxicology.View full abstractDownload PDF (6642K)
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