About the filterpaper electrophoresis of proteins, we come to a conclusion as the following, from the result of experiment, ussing serum (plasma) proteins as trial material after studing the provision of experimentation, material of experimentation according to the method of the experimentation.
1. We find inportant condition in the position on which we put the trial material, how much to have an overdose of it, and how fresh to have it.
2. The quality of the filterpaper has a close relation with the result of electrophoresis of proteins. We find the good result in the filterpaper of Whatman No. 1, Schleicher & Schüll No. 2043 a, b or Toyo filterpaper corp. No. 51, 51a.
3. About to the condition of the electrophoresis, the voltage, the electric current and the time of the phoresis are concerned each other. As to decide strictly on these point, we find some deficult problem, but we know that the condition of the phoresis had decided naturally, if we have the fixed filterpaper and buffer.
4. We find the best conditioh in about 7cm in phoresis distance and in 100-110°C, 20 minutes in the method of the desiccate as the same as we find from the result of experiment as to the phoretic distance and the desiccate method.
5. 20 minutes are the best in the time of dyeing so for as Amido black 10B is concermed.
We think as if We will find some deference between Amido black 10B and B. P. B. dyeingin in the result of the some amount, but it is not only clear above mentioned writting in this point of view but it is also necessary to study in future.

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Protein, lipoprotein (Lp) and gfycoprotein (Gp) were studied electrophoretically in liver disease and experimental liver impairment which was induced in rabbits. Total protein (T.p), cholesterol (Ch) and protein-bound polysaccharide (PBP) were also studied.
Rabbits used in this experiment were injected with Indian ink and CCl₄. Liver diseases studied were hepatitis, liver cancer and liver cirrhosis.
1. Changes, which were related to the extent or progress of liver impairment when the rabbits were treated with Indian ink, were a decrease in the T. p and albumin levels and a corresponding increase in the γ-globulin and α₁-globulin fractions. In the case of the Lp level, there was an increase in the total Ch level and a decrease in the β- and γ-Lp fractions. The esterified cholesterol level was in parallel to the total cholesterol level. In the case of the Gp level, the PBP level did not undergo so great changes. A tendency for the α₁-Gp fraction to increase was interesting.
2. In the case of CCl₄ injection, changes which were related to the extent of liver impairment were that the T. p level showed an increase after a temporary decrease and that the albumin fraction followed opposite courses to the T. p level. The γ-globulin fraction was corresponding to the albumin fraction and showed almost the same tendency as observed in the case of Indian ink. An increase in the α₂-globulin fraction was observed in patients who had died. In the case of the Lp level, the total and esterified cholesterol levels showed a sudden decrease after the injection, and the esterified cholesterol/total cholesterol ratio also exhibited a decrease. On the other hand, β-Lp fraction increased after it decreased. In the case of the Gp level, the PBP level did not undergo so great changes. It showed a temporary increase in some cases, but its decreasing tendency was noticed. A tendency forthe α₁-Gp fraction to increase was observed.
3. In clinical experiments a decrease in the T. p level and the albumin fraction as well as an increase in the γ-globulin fraction was perceived in many instances. The decreasing rate of the T. p level was the smallest in liver cancer, followed by liver cirrhosis and hepatitis. The decreasing rate of the albumin fraction was greater in liver cirrhosis than in liver cancer, and the α₂-globulin fraction showed a high value in liver cancer. In the case of hepatitis, a difference according to the intensity of its symptoms was reflected in the T. p level. As foa the Lp level, an increase in the total cholesterol and esterified cholesterol levels and the β-Lp fraction occurred in the advanced stage of icterus in hepatitis, while there was a decrease in these levels in cases of great liver function impairment. The total cholesterol level was high in cases of liver cirrhosis which followed favorable courses, whereasit was low in cases which followed unfavorable courses ann especially in cases of death. It showed a decrease in the progressive stage of liver, cancer. In the case of the Gp level, the PBP level was high in cases of hepatitis where jaundice was marked, while it was low in cases where liver function impairment was great. In the stadium acme of hepatitis, there was an increase in the α₁-Gp fraction. In liver cancer, the PBP level and the α₁- and α₂-Gp fractions showed an increase and these exhibited a greater increase along with the progress of this disease. The PBP level was low in cases of liver cirrhosis which followed unfavorable courses, while it was high in cases Which followed favorable courses. The high α₁- and α₂-Gp fractions were reduced by medical treatment, whereas the low ones showed a tendency to be increased.

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For the purpose of electrophoretically studying the relation between the general and the local abnormal metabolism in malignant tumor, changes in the levels of proteins, glycoprotein and protein-bound polysaccharide from the tissue were followed up in comparison with findings of serum proteins in the tissue of the stomach removed from cases of gastric cancer and ulcer and also in the tissue of the stomach excised from gastric sarcoma-implanted and Yoshida sarcoma-implanted rats. The following conclusions were thus reached:
1. The characteristic findings of proteins from the tissue of cases of tumor were a very remarkable decrease in the albumin fraction, an increase in the α₁-globulin fraction and the α₁-glycoprotein fraction and a significant increase in the protein-bound polysaccharide level. Though these findings showed the same tendency as observed in the findings of serum proteins, they appeared earlier than the latter and were considered to be characteristic of the tissue from cases of malignant tumor.
2. This pronounced decrease in the albumin fraction was a main factor in a decrease in the serum albumin and total protein levels.
3. An increase in the protein-bound polysaccharide level seemed to be chiefly responsible for an increase in the serum protein-bound polysaccharide level.
4. An increase in the α₂-globulin fraction and the α₂-glycoprotein fraction which was observed in serum was a finding which was equally met with in cases of phthisis where proliferation or inflammation or degeneration of cells occurred. The increase seemed to be parallel to the extent of changes in these phenomena.
5. In other words, the foregoing findings can be regarded as indicating (1) the general and local abnormal metabolisms in the cancer-carrying organism, (2) the close relationship between both metabolisms and (3) the process in which local changes in malignant tumor gradually pass into findings of serum proteins.

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For the purpose of making a diagnosis of arteriosclerosis clinical and biochemically, patients with hypertension, diabetes and kidney disease were loaded with dietetic fats in an attempt to ascertain time-to-time changes in the turbidity of serum, the phospholipid level, the cholesterol level, the lipoprotein level, the protein level and the glycoprotein level. Healthy persons were likewise treated with fats for comparison with a group of patients. The following conclusions were reached:
1. These changes in the group of patients were obviously different from those in the healthy persons and they varied according to the type of disease. The lipemia-clearing activity, as seen from a standpoint of turbidity, was poor for cases of hypertension and particularly those with symptoms of lipemia. The lipemia-clearing activity of diabetic patients was between those of people with and without symptoms of this disease.
2. In the healthy persons, the phospholipid level showed a temporary increase after loading, but returned rapidly to normal. In the group of patients, the increasing rate of the level was great and the return of the level to normal took place slowly. The extent to which the level returned to normal was more considerable in people with symptoms of hypertension than in those without such symptoms.
3. The effects of lording on the cholesterol level did not so clearly manifest themselves in the healthy persons, but hypertensive and diabetic patients showed a gradual increase in the level. The increase in cases of nephrosis took place more rapidly.
Changes in each fraction of lipoprotein following loading were violent in the group of patients. An increase in the γ-lipoprotein fraction subsequent to loading was significant in cases of hypertension, diabetes and nephrosis. After loading the behavior of the fraction in cases of nephrosis was different from that in cases of nephritis.
4. Changes in each fraction of protein and glycoprotein were followed up in connection with changes in the components of lipids. As a resut it was found that there was a clear-cut difference in the behavior these fractions between healthy persons and a group of patients, and that hypertensive and diabetic patients showed almost the same tendency. Contrary to healthy persons, hypertensive and diabetic patients showed elevated α₁-globulin levels and the increasing rate was greater in diabetic patients. Unlike healthy persons, hypertensive patients exhibited a decrease in the α₁-and α₂-glycoprotein fractions, while an increase in the α₂-glycoprotein fraction occurred in diabetic patients.

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