生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
8 巻, 1 号
選択された号の論文の5件中1~5を表示しています
  • 平井 秀松
    1961 年 8 巻 1 号 p. 1-8
    発行日: 1961/07/15
    公開日: 2009/03/31
    ジャーナル フリー
  • 血清蛋白質及び血清複合蛋白質に関する臨床的研究 第15報
    金田 春雄
    1961 年 8 巻 1 号 p. 9-33
    発行日: 1961/07/15
    公開日: 2009/03/31
    ジャーナル フリー
    An increase in diseases due to arteriosclerosis, together with malignant tumor, has been remarkable in recent years, and studies on arteriosclerosis have become active. Thus it has been clarified that the disease is closely related to abnormal lipometabolism in the human body. As a series of clinobiochemical studies on serum proteins and compound proteins which have been carried out in this clinic for several years, the present author studied abnormal lipometabolism in arteriosclerosiss from a viewpoint of alternations of findings in the serum of hypertensive patients and the serum of cholesterol-fed rabbits. In this connection, observation was made, with the following conclusions, on alternations of findings in proteins and glycoprotein as well as yearly alternations of findings in serum proteins and glycoprotein.
    1. In rabbits with hypercholesteremia, the concentration of blood cholesterol showed no correlation to the β-lipoprotein fraction, and there was an increase in lipoprotein of a very low specific gravny.
    2. A slight decrease in the serum total protein, a decrease in albumin and an increase in the globulin fraction took place in essential hypertension, regardless of whether or not it was complicated by arteriosclerosis.
    3. The total cholesterol level in arteriosclerosis, hypertension and old age was high in this order. The level is useful for determining whether or not essential hypertension is complicated by arteriosclerosis. In a group of diseases, the ester cholesterol/total cholesterol ratio exhibited a decrease. In this case there was a correlation between the total cholesterol level and the β-lipoprotein fraction.
    4. In old age, the γ-lipoprotein fraction showed an increase, while the α- and β-lipoprotein fractions exhibited an increase. In both groups a decrease in the α-lipoprotein fraction took place to almost the same extent. The maximal level of the β-lipoprotein fraction occurred in arteriosclerosis. The γ-lipoprotein/α-lipoprotein ratio and the β-lipoprotein/α-lipoprotein ratio showed a striking increase in old age and arteriosclerosis, respectively. The β+γ-lipoprotein/α-lipoprotein ratios for both were 3.2 and 3.1; almost no difference in the ratio was observed.
    5. An increase in the α2-glycoprotein fraction was significant in arteriosclerosis; the P. B. P. showed a high level in cerebrosclerosis. The P. B. P. level and the α2-glycoprotein fraction for hypertensive patients, slightly hypertensive patients and patients with arteriosclerosis gave high values in the reverse order. There was a slightly significant correlation between both.
  • 湊 栄一
    1961 年 8 巻 1 号 p. 34-57
    発行日: 1961/07/15
    公開日: 2009/03/31
    ジャーナル フリー
    Differences in changes in the Iipids constituents of serum between yound and old people following dietetic fat loading were studied. Also, the differences according to the presence or absence of hypertension and angiosclerotic changes were observed. The effects of Lipostabil, a lipophil substance, on the changes following fat loading were studied in cases of cerebral apoplexy. The results thus obtained are as follows:
    1. From the standpoint of changes in the turbidity, the lipemiaclearing activity of old age was poor and this disturbance was remarkable particularly in the subjects with a symptom of hypertension.
    2. The phospholipid level in the blood showed a temporary increase and it decreased in the course of the lipid-clearing process. In hypertensive patients the process took place slowly and the utilization of the process was insufficient. Accordingly, the phospholipid level gradually increased together with the cholesterol level after the fat loading, and the recovery of these levels to normal was slow. Healthy persons showed no definite changes in the cholesterol level due to the loading.
    3. Neither the Ch/P ratio nor the β/α ratio is of an important significance, but the γ/α ratio has a significance. This is related to an increase in the γ-lipoprotein fraction. After the loading the increasing rate was for young people, old people, hypertensive patients and persons with angiosclerotic changes in this order; and the recovery of the fraction to normal was slow in the same order.
    4. When a single injection of 5cc of Lipostabil was made, the phospholipid level showed a marked increase and the ch/p ratio had a tendency to decrease.
    5. When 10cc of Lipostabil was injected, there was a striking increase in the phospholipid level. The cholesterol level underwent the same changes as observed in healthy persons whose cholesterol level showed a few tendencies to increase.
    6. When 5cc of Lipostabil was injected continually, the cholesterol level had a few tendencies to increase. As for lipoproteins, the α-lipoprotein fraction, the γ-lipoprotein fraction and the γ/α ratio underwent the same changes as took place in healthy persons who had a few tendencies to show a decrease in the first fraction and an increase in the two latter levels. Improvement in the levels and particularly the cholesterol level was more remarkable than when a single injection of 10cc was made. However, the phospholipid level behaved almost as similarly as before the injection was made.
  • 山本 敏雄
    1961 年 8 巻 1 号 p. 58-76
    発行日: 1961/07/15
    公開日: 2009/03/31
    ジャーナル フリー
    In recent years a diphenylamine-positive substance has gradually acquired considerable clinical importance as an acute phase reactant. Although that substance is generally regarded as sialic acid, much still remains unexplained as to the nature of the diphenylamine reaction.
    The present study was made, with the following results, to investigate the clinical significance of that reaction and its nature in relation to other test results:
    1) The value of the reaction in healthy subjects was 0.175±0.020 and there was no difference according to sex and age.
    2) Patients with internal diseases who exhibited high values comprised cases of malignant tumors, cholecystitis, pleurisy, pneumonia, rheumatic fever, and rheumatoid arthritis in the stadium incrementi, whereas cases of hepatitis and hepatic cirrhosis showed low values.
    3) In general the reaction was closely related to the destruction, growth and inflammation of tissue and was in good agreement with the intensity and course of a disease. It is a useful aid in the assessment of therapeutic effects or prognosis.
    4) The reaction was clearly correlated with mucoprotein, protein bound polysaccharide and C-reactive protein. A slight parallelism existed between the reaction and the blood sedimentation value, but the cephalin cholesterol flocculation test, the antistreptolysin O test and the icteric index were not related to the reaction.
    5) On paper electrophoresis the reaction showed a correlationship with α2- and α1-globulin and a more significant correlationship with α1-and α2-glycoprotein, while it had no correlation with the lipoprotein fraction. Accordingly, the diphenylamine-positive substance can be thought to be protein bound polysaccharide which is located mainly in the α-globulins.
    6) It can be presumed that the reactant might originate in the liver, tissue from inflammation and tumors.
  • 1961 年 8 巻 1 号 p. 77-85
    発行日: 1961/07/15
    公開日: 2009/03/31
    ジャーナル フリー
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