Two-dimentional crossing paper electrophoreses of human blood serum against Protein J contained in the extract from Jack bean were carried out, and the changes in the “crossing diagram” obtained from 159 patients with various diseases were studied in comparison with those of 40 normal subjects.
1) Human sera, except in some diseased cases, showed crossing diagrams consisting of three peaks, which correspond to three globulin fractions, α
1-, α
2- and β-globulin. The peaks were formed due to the precipitation reaction between Protein J and these serum proteins.
2) Sera from 58 patients showed crossing diagrams which can not be differentiated from those of normal sera. On the other hand, sera from 101 patients showed clearly different crossing diagrams from those of normal subjects. The changes in the crossing diagrams were classified into 7 types.
3) Sera from 5 patients (including two multiple myeloma, one leucosarcoma, one gastric ulcer with gastric ffbroma, and one liver cirrhosis) showed crossing diagrams with an unusual fourth peak besides the three.
4) Specific correlation between the type of the changes in crossing diagrams and the diseases was hardly observed, although in nephrotic syndrome diagrams were marked with pronounced elevation of α
2 peak.
5) Relations between the relative concentrations of serum α
1-, α
2- and β-globulin fractions determined by the usual paper electrophoresis and the changes in crossing diagrams were investigated. In most cases they could be correlated, but in some cases correlation could not be observed.
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