This work was concerned with the characterization of nonkeratinous and keratinous proteins of human hair eluted by permanent wave lotion I (PWL-I). Polyacrylamide gel electrophoresis, infrared spectroscopy and amino acid analysis method were applied.
From these examinations, the following results were obtained. (1) Nothing was eluted from human hair by hot water (60°C, 3hr treatment), whereas some extracts were obtained using alkaline solution, surfactant solution, or PWL-I (permanent wave lotion I). (2) The mass of the proteins extracted by PWL-1 accounted for about 1% of the total mass of the hair and tended to increase with an increase in pH. (3) It was confirmed that the molecular weights ranged from 5, 000 to 65, 000 by SDS PAGE. (4) The amino acid composition of the PWL-1 extracts was similar to that of colagenous proteins which was unexpected. The composition was diffrent from that of intercuticular material, δ, eluted by formic acid. Therefore, we suspected that the PWL-1 extract was eluted from the δ layer among the cortical cells.
In addition, it was clarified that a part of keratinous protein was extracted from hair which was damaged by repeated treatments of PWL or breached by hydrogen peroxide and ammonia water. It is thought to be eluted from the matrix in the cortical cell which is composed of amorphous keratin of high sulfer content.
These results will help clarify the mechanism of the elution of intercellar material of humann hair and the mechanism of hair damage by external treatments.

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The purpose of skincare is to make the skin keep and improve to physiologically good condition. So, we hypothesized that it was the most important thing for effective skincare to fit in with individual skin condition, which was applied by the skincare system based on scientific and detailed skin classification.
The women of 113 were divided to two groups, “individual skin fitting group” whose skin condition were measured by scientific methods and classified, and “control group” who were treating their skin by oneself. We evaluated effectiveness of the system by means of the corneous cell examinations of each skin of two groups before and after the use test of 6 weeks.
As the result, individual skin fitting group showed the tendency of improvement on Thick Abration and regularity of corneous cell arrangment in comparison with control group.
Therefore, we think it substantiate the effectiveness of the system to fit in with individual skin condition.

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New amphoteric surfactants, dimethylaminoalkanesulfonates (DAMS), were synthesized by a ring opening reaction of alkylsultones, obtained by the reaction of α-olefin with sulfurtrioxide, with dimethylamine.
Judging from the results of ¹H-NMR measurement, DAMS seemed to take a zwitterionic form in the region of pH<8 and an anionic one in the region of pH above 12. Results of ESR measurement using a spin probe, which is known to be capable of locating the inner part of micelle, showed smaller hyperfine splitting constant (a_N) in DAMS solution than those found in common surfactants, indicating that the micelle interior of DAMS has hydrophobic nature.
Surface activities of DAMS were increased with the decrease of distance between amino group and sulfonate group. On the contrary, Krafft points of 4-DAMS were lower than those of 3-DAMS. The solubilizing and emulsifying power of DAMS to nonpolar materials exceeded AOS and other amphoteric surfactants with corresponding alkyl chain length. DAMS also had a favorable lime soap dispersing power. Foamability of DAMS was similar to the common surfactants when it is applied to shampoo formulation. These performance of DAMS are considered to be due to zwitterionic form with hydrophobic nature under neutral or acidic condition.
Denaturing power to Bovine serum albumin, so called skin roughness, and primary skin irritation of DAMS were significantly lower than those found in conventional mild surfactants such as monoalkylphosphate and acyl glutamate.
In conclusion, DAMS is an advantageous amphoteric surfactant for cosmetics and toiletries having superior surface activities and practical performance as well as physicological mildness. Furthermore, DAMS has an economical benefit since it can be derived readily from solfonation product of α-olefin.

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A lecithin sol containing magnesium ascorbate phosphate (3.0wt%) and tocopheryl acteate (0.1wt%) was prepared with a mixture of hydrogenated soya lecithin, cholesterol and a certain nonionic surfactant by a homogenizer.
The lecithin sol was stable for over two months at 50°C.
The viscosity of the lecithin sol was 2.2cP at 25°C by the Brookfield type viscometer, which is the same value as a lecithin sol without magnesium ascorbate phosphate.
The mean diameter of the lecithin sol measured by both the coulter counter N4 and the Nicomp was about 100nm and did not change for over two months at 50°C.
The encapsulation rate of magnesium ascorbate phosphate mearsured by both the centrifuge and the chromatography method was found to be in the range of 16-34%.

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