The recent development of a confocal microscopic (CM) technique for skin imaging enabled the examination of the internal structure of human skin at a cellular level. We used CM (Vivascope 1000, Lucid Co., USA) to investigate the structural and morphological changes with skin depth in a normal volunteer as well as the epidermal thickness and the change of epidermal-dermal junctional undulation in experimentally induced rough skin by SDS (sodium dodecyl sulfate) or tape-stripping, We also examined those of change in sun-exposed and sun-protected areas. In order to investigate the epidermal-dermal undulation we reconstructed the 3D structure of skin to cumulate the images of horizontal section and finally extracted the undulation from the vertical section images using the Snakes method. The morphology of keratinocyte changed drastically with skin depth. The average projected area in basal cells was about 100μm
2 and that at the surface of stratum corneum was about 1200μm
2. These results show that the keratinocytes become more than 10 times flatter at the skin surface during differentiation and keratinization. Both treatments of SDS and tape-stripping made the epidermis thicker (more than two times compared with control) and these effects continued until 25 days after treatment. On the other hand, the skin surface topolography returned almost to its initial state after 25 days. Our results suggest that the superficial recovery of stratum eorneum from external stimuli is comparatively fast but the effeet on the internal condition of the skin lasts longer. Epidermal thickness and the undulation of dermal papillae are quite different in sun-exposed (cheeks, the dorsal forearm) and sun-protected (the volar upperarm) area. The epidermis in the cheek site is thicker than in the upperarm and the epidermal-dermal junction is clearly undulated compared with other areas. From there results it was concluded that the
in vivo confocal microscope is a useful instrument to examine the internal structure of human skin non-invasively and to evaluate the effects of cosmeceutical ingredients on the skin.
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