Salmonella enterica serover Enteritidis (SE) strains isolated in Osaka city were genotyped by pulse field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) methods. Fifty-five isolates from 16 food-borne outbreaks, 2 sporadic isolates and an environmental isolate were analyzed. By PFGE method, they were divided into 8 types. Some isolates from different outbreaks showed identical PFGE patterns. Twenty isolates from 11 food-borne outbreaks and an environmental isolate, which showed 4 PFGE patterns, were further analyzed by more resolutive AFLP method. They were typed into 2 patterns. These results suggest that AFLP method is not suitable for genotyping of SE isolated in Osaka city as PFGE method is not. The possibility that identical clones of SE spread in Osaka city has yet to be elucidated.
Change of fungal and bacterial counts inoculated to towel and subjected to treatment by clothes dryer or natural sun-drying was examined. No significant difference was found in sterilizing effect on fungi and bacteria by drying of towel in the sun or in the shade. Sterilizing effect on C. cladosporioides and P. digitatum was higher in towel sun-dried in summer than in winter, and in towel dried by clothes dryer at higher temperatures than at lower temperatures. E. coli as well as T. rubrum seems to be killed with loss of moisture in towel regardless of temperature at which towel is dried.