The present study investigated the effect of photoperiod on the process of cold acclimation in rats. Male rats were divided into two groups, each of which was subjected to one of two photoperiod regimes, i. e., long photophase (LD cycle 18: 6 h; LP) or long scotophase (LD cycle 6: 18 h; LS) . The ambient temperature (T
a) was constantly maintained at 23°C throughout the experiments (warm exposure) or was lowered to 5°C at the 14th day after starting the photoperiod regime (cold exposure) in each group. Body mass (BM), food consumption (FC), thermogenic response to noradrenalin (NA response) and plasma levels of free fatty acid (FFA), triglyceride (TG) and glucose (G) were measured at intervals of 3-10 days. The BM of the LP rats was significantly greater than that of the LS rats regardless of the cold exposure. The photoperiod itself did not affect NA response or plasma levels of FFA, TG and G. In the LS rats, the NA response was significantly enhanced from the third day after starting the cold exposure, whereas in the LP rats, the response was enhanced 10 days after commencing the cold exposure. Additionally, the NA response of the LS rats was significantly greater than that of LP rats between 21-28 days after starting the cold exposure. During the same period, plasma levels of FFA and TG of the LS rats were significantly higher than those of the LP rats. These results suggest that the long scotophase facilitates the process of thermogenic adaptation to cold, possibly, mediated by modification fat metabolism.
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