SEISAN KENKYU
Online ISSN : 1881-2058
Print ISSN : 0037-105X
ISSN-L : 0037-105X
Volume 69 , Issue 3
Showing 1-15 articles out of 15 articles from the selected issue
Introduction to Special Section
Research Review
  • Masahiro SATO, Akiko KUMADA, Kunihiko HIDAKA, Toshiyuki HIRANO, Fumito ...
    2017 Volume 69 Issue 3 Pages 107-113
    Published: May 01, 2017
    Released: May 30, 2017
    JOURNAL FREE ACCESS

    Electron transfer and carrier transfer (CT) in biomolecules, organic semiconductors and insulators has attracted considerable attention for industrial applications. However, it is still non-trivial to model CT through large and highly complex biological matter and polymers. In this contribution, the relation between morphology and hole mobility in polyethylene (PE) is studied without adopting ad hoc assumptions. Computed hole mobilities in crystalline and amorphous PE was in reasonable agreement with experimental data. The impact of the morphology of polymers on carrier transfer characteristics is quantitatively assessed.

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  • Angela CHOW, Yoshiho IKEUCHI
    2017 Volume 69 Issue 3 Pages 115-121
    Published: May 01, 2017
    Released: May 30, 2017
    JOURNAL FREE ACCESS

    Regulation of protein synthesis is critical for neuronal development and functions. Protein synthesis dynamics is uniquely complex in neurons due to their elaborated cellular shape and activity-dependent response. Here we focus on protein synthesis in neurons and we review its function, associated diseases, and analysis technologies. Various new approaches for analysis of protein synthesis dynamics have been developed, and facilitating research on protein synthesis regulations in neurons.

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  • Haruko TAKAHASHI, Keisuke KATO, Masayoshi KOBAYASHI, Kenji UEYAMA, Yuk ...
    2017 Volume 69 Issue 3 Pages 123-126
    Published: May 01, 2017
    Released: May 30, 2017
    JOURNAL FREE ACCESS

    Three-dimensional (3D) in vitro microvasculature in a microdevice was developed as a physiologically relevant model of angiogenesis. The angiogenic process was monitored using stage-top optical coherence tomography (OCT). The OCT allows non-invasive monitoring of the 3D structures of the prepared host microvasculature and sprouted neovasculature without fluorescence staining. The obtained OCT cross-sectional images capture 3D features of the angiogenic sprouting process and provide information on the dynamics of luminal formation. The stage-top OCT system is a simple and convenient monitoring method that enables to visualize the in vitro dynamics and morphological changes of 3D cultured cells in live.

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  • Ryo USUBA, Joris PAUTY, Haruko TAKAHASHI, Junichi SUEHIRO, Yukiko T. M ...
    2017 Volume 69 Issue 3 Pages 127-132
    Published: May 01, 2017
    Released: May 30, 2017
    JOURNAL FREE ACCESS

    Blood vessel has a barrier function that regulates the flux of substances in and out the vessel—a phenomenon known as vascular permeability. This function is ensured by an endothelial cell monolayer that forms the most inner layer of a blood vessel. During inflammation, inflammatory factors act on endothelial cells to increase vascular permeability. In various diseased conditions, the barrier function is disrupted. Thus an effective method to evaluate vascular permeability is required. However, conventional methods suffer limitations such as difficulty for assessing detailed physiological process. In this article, we introduce a recently developed in vitro 3-dimensional blood vessel model to assess vascular permeability in a biomedical context.

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Research Flash
  • Hiroko RYU, Yoshiro MORITA, Yukiko T. MATSUNAGA
    2017 Volume 69 Issue 3 Pages 133-136
    Published: May 01, 2017
    Released: May 30, 2017
    JOURNAL FREE ACCESS

    "Architecture" is generally translated as "kenchiku", but it is also used in various research fields. In order to find how the word of "architecture" was used, we investigated the following three items in the Web of Science of academic literature search service: (i) the number of documents including "architecture" in their titles, (ii)the research fields of documents including "architecture" in the title, and (iii) the overlap of research fields of documents including "architecture" in the title. We obtained the knowledge that the word of "architecture" was mainly used in the field of "Arts, Humanities" in the early days, but in recent years it was used in the field of "Technology".

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  • Saeko TACHIKAWA, Shohei KANEDA, Momoko KUMEMURA, Rui SATO, Teruo FUJII ...
    2017 Volume 69 Issue 3 Pages 137-140
    Published: May 01, 2017
    Released: May 30, 2017
    JOURNAL FREE ACCESS

    In this research, we developed a microfluidic in vitro cancer invasion model which takes into account both intercellular adhesion and cell-cell interaction existing in 3D tumor cell clusters. The device has 2 parallel main channels connected with 10 orifices which have taper-shaped structures. The diameters of wider openings of the orifice and its narrower pore are approximately 80 μm, and 10 μm respectively. The wide opening allows several cells to be packed in the orifice and to touch each other tightly. The device made of PDMS was fabricated through two-step softlithography using an SU-8 mold produced by a 3D lithography technique. In device evaluation, PC-3 cells (a prostate cancer cell line) introduced from one main channel were packed into the orifices using a flow towards the other main channel generated by hydrostatic pressure difference. Then by filling the other main channel with a medium containing invasion-inducing factors, an invasion cell moved through the pore along the laminin-coated wall of the orifice was successfully observed on the device.

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  • Naotake KASAI, Momoko KUMEMURA, Shohei KANEDA, Teruo FUJII, Hiroyuki F ...
    2017 Volume 69 Issue 3 Pages 141-143
    Published: May 01, 2017
    Released: May 30, 2017
    JOURNAL FREE ACCESS

    Our final target is establishing a method using a MEMS device (Silicon nanotweezers: SNT) to conduct mechanical characterization of single tumor cells followed by single-cell gene expression analysis. First, we confirmed that the SNT can be used for both mechanical characterization of a tumor cells and manipulation for cell lysis procedure prior to gene expression analysis. Second, we checked effects of using the SNT for single cell manipulations on required reactions for gene expression analysis. The results obtained in this study suggest using the SNT does not cause inhibition for the required reactions.

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Introduction to Special Section
Research Flash
Research Review
  • Fumiyuki FUJII, Kazuyuki AIHARA
    2017 Volume 69 Issue 3 Pages 155-158
    Published: May 01, 2017
    Released: May 30, 2017
    JOURNAL FREE ACCESS

    Lightning causes damage of our infrastructures, such as power grids and communication systems. In order to protect such damage, knowledge on characteristics of lightning is essentially important. Therefore observation data of LLS (Lightning Location System), which measures electro-magnetic waves propagated from lightning discharges, are statistically analyzed. In this report, distribution of inter-stroke intervals (ISIs), multiplicity of flashes, and their seasonal variations are evaluated.

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Research Flash
Field Report
  • Mihiro NOMURA, Michio MURAKAMI, Yuya ONO
    2017 Volume 69 Issue 3 Pages 171-175
    Published: May 01, 2017
    Released: May 30, 2017
    JOURNAL FREE ACCESS

    There has been growing interest in the relationship between climate change and crime. This study investigated the relationship between temperature and crime rate from 1967 to 2011 in Tokyo. During the summer, rape and obscenity were frequent. The monthly crime rate ratios (i.e., the ratio of crime rate in a particular month of a given year to the average monthly crime rate in that given year) of rape and obscenity rose significantly with increases in average temperature during the winter and summer, respectively. An increase in temperature could potentially increase the risks of rape and obscenity.

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