The Closed Ecology Experiment Facilities (CEEF) including Plantation Module, Animal Breeding & Habitation Module, Geosphere Module, Hydrosphere Module, and Material Circulation Systems has been constructed for the experiment of material circulation among plants, breeding animals, humans (crew of the CEEF), and creatures in land and ocean environments. The crew will have to do a lot of work to produce their food, and to take care of the animals and plants. When workload exceeds the crew capability, some assistants will enter into the CEEF to help them. In this case, if the assistants take O
2 from and expel CO
2 into the CEEF atmosphere, the material circulation will be disturbed to some extent. The Closed Work Suits (CWS) are to protect the material circulation in the CEEF against these disturbances caused by respiration of the assistants. The development of the CWS started in 1995, and finished in 2000. A closed circuit breathing apparatus can be used in a stand-alone mode as the basic CWS. This is easy to use, but a little of breathing gas would inevitably leak from the mask. For more accurate experiments, the integrated system of the breathing subsystem and the airtight suit is to be used, because the leaked gas from the mask is kept in the airtight suit. In the case that a wearer's metabolic heat is accumulated in the airtight suit, the cooling subsystem is needed to remove the metabolic heat. So, each of the CWS consists of three subsystems, a breathing subsystem that supplies O
2 and removes CO
2 from the breathing gas, a cooling subsystem that removes the metabolic heat from wearer's body and an airtight subsystem. The CWS must continue to perform for more than 1 hour, because it is planned that assistants will work continuously for 1 hour. Based on studies, manufacturing prototypes and man-rated experiments, characteristic requirements for the CWS have been decided. The breathing subsystem can supply O
2 and remove CO
2 at the rate of 2.7 × 10
-2 mol min
-1, and the cooling subsystem can cool down the circulation water at 300 W maximum during the CWS operation for 1 hour. The key of the development of the CWS for practical use was the weight. The weight of the functional model, that was fabricated to satisfy the characteristic requirements, was more than 51.5 kg. By redesigning the cooling subsystem, the weight of the operational model was reduced to 22.5 kg (Weight loaded on wearer's back with harness is 15.2 kg). The operational model worked well throughout all man-rated tests and the safety of the model was completely verified.
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