A feeding experiment was carried out for tiger puffer,
Takifugu rubripes, with a recirculating aquaculture system of 37 m
3 in total water volume. The system consisted of a fish tank of 27 m
3 culture water, drum screen filter, submerged biofilter, temperature control unit, oxygen and air generator and diffusers, and UV sterilizer. Underground seawater of salinity 23 ppt after filtered through 25
μm porous media and UV treatment was used as culture water with daily exchange of 2 m
3 to keep nitrate level under 100 mg-N/L. At the start of the experiment, 1,000 fish of 6.9 g in average body weight were introduced to the fish tank and fed commercial pellet once to three times daily. Fish grew to an average weight of 960 g after 490 days of rearing with good survival rate of 90%. Total fish weight in the system was 850 kg, and the final stocking density per unit water volume in the culture tank reached to 30 kg/m
3 at the end. Nitrification operated successfully and ammonia and nitrite of the rearing water were maintained less than 2 mg-N/L almost through the experimental period. These results demonstrate that tiger puffer can be effectively produced in low saline seawater recirculation system with partial exchange of culture.
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