The hourly CO
2 assimilation rate of lettuce plants (
A) in a closed system with artificial lighting (CSAL) for commercial production was estimated based on the CO
2 balance. Constant values for workers' respiration rate and number of air exchanges per hour were used to simplify the estimation.
A was varied during the experimental period due to the variation in the leaf area of lettuce plants under lighting (
LAL). However, the estimates of
A contain inaccuracies. The percent error in
A due to the variation in number of air exchange per hour can range from - 8 % to 20 %. Besides, the variation in the workers' respiration rate also can cause inaccuracy of
A. The results indicate that
A can be estimated based on the CO
2 balance. The number of air exchanges per hour and estimation of workers' respiration rate must be accurate to improve the accuracy of
A.
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